标签归档：triphosphate

ddCTP [2′,3′-Dideoxycytidine-5′-triphosphate] 货号17207-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ddCTP [2′,3′-Dideoxycytidine-5′-triphosphate]

ddCTP [2′,3′-Dideoxycytidine-5′-triphosphate]

ddCTP [2',3'-Dideoxycytidine-5'-triphosphate] 货号17207 货号 17207 存储条件 在零下15度以下保存, 避免光照
规格 1 umole 价格 2472
Ex (nm) Em (nm)
分子量 451.16 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17207

产品名称：ddCTP [2′,3′-Dideoxycytidine-5′-triphosphate]

规格：1 umole

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：451.16

溶剂：水

 

产品介绍

Sanger测序，也称为链终止法，是一种基于DNA聚合酶选择性掺入链终止双脱氧核苷酸（ddNTPs）的DNA测序技术。它是由Frederick Sanger及其同事于1977年开发的。尽管新的NGS技术由于其较高的通量能力和较低的每份样品成本而在临床研究实验室中变得很普遍，但Sanger测序仍具有99.99％的准确度，仍然是临床的“黄金标准”研究排序。 dd-CTP是执行Sanger测序的四个关键ddNTP组件之一。

 

参考文献

Involvement of DNA polymerase beta in DNA replication and mutagenic consequences.
Authors: Servant, Laurence and Bieth, Anne and Hayakawa, Hiroshi and Cazaux, Christophe and Hoffmann, Jean-Sébastien
Journal: Journal of molecular biology (2002): 1039-47

Fluorescence-based DHPLC for allelic quantification by single-nucleotide primer extension.
Authors: Kosaki, K and Yoshihashi, H and Ohashi, Y and Kosaki, R and Suzuki, T and Matsuo, N
Journal: Journal of biochemical and biophysical methods (2001): 111-9

Denaturation fingerprinting: two related mutation detection methods especially advantageous for high G + C regions.
Authors: Liu, Q and Weinshenker, B G and Wingerchuk, D M and Sommer, S S
Journal: BioTechniques (1998): 140-7

Synergistic inhibition of HIV-1 reverse transcriptase by combinations of chain-terminating nucleotides.
Authors: Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E
Journal: Biochemistry (1997): 13223-31

A fidelity assay using “dideoxy” DNA sequencing: a measurement of sequence dependence and frequency of forming 5-bromouracil X guanine base mispairs.
Authors: Lasken, R S and Goodman, M F
Journal: Proceedings of the National Academy of Sciences of the United States of America (1985): 1301-5

说明书
ddCTP [2′,3′-Dideoxycytidine-5′-triphosphate].pdf

ddTTP [2′,3′-Dideoxythymidine-5′-triphosphate] 货号17208-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ddTTP [2′,3′-Dideoxythymidine-5′-triphosphate]

ddTTP [2′,3′-Dideoxythymidine-5′-triphosphate]

ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] 货号17208 货号 17208 存储条件 在零下15度以下保存, 避免光照
规格 1 umole 价格 2472
Ex (nm) Em (nm)
分子量 466.17 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17208

产品名称：ddTTP [2′,3′-Dideoxythymidine-5′-triphosphate]

规格：1 umole

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：466.17

溶剂：水

 

产品介绍

Sanger测序，也称为链终止法，是一种基于DNA聚合酶选择性掺入链终止双脱氧核苷酸（ddNTPs）的DNA测序技术。它是由Frederick Sanger及其同事于1977年开发的。尽管新的NGS技术由于其较高的通量能力和较低的每份样品成本而在临床研究实验室中变得很普遍，但Sanger测序的准确度仍为99.99％，仍然是临床的“黄金标准”研究排序。 dd-TTP是执行Sanger测序的四个关键ddNTP组件之一。

 

参考文献

Strong positional preference in the interaction of LNA oligonucleotides with DNA polymerase and proofreading exonuclease activities: implications for genotyping assays.
Authors: Di Giusto, Daniel A and King, Garry C
Journal: Nucleic acids research (2004): e32

Detection and genotyping of human papillomavirus DNA in cervical cancer tissues with fluorescence polarization.
Authors: Gao, Yan-E and Zhang, Ju and Wu, Jing and Chen, Zhong-Can and Yan, Xiao-Jun
Journal: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica (2003): 1029-34

Dye structure affects Taq DNA polymerase terminator selectivity.
Authors: Brandis, J W
Journal: Nucleic acids research (1999): 1912-8

Synergistic inhibition of HIV-1 reverse transcriptase by combinations of chain-terminating nucleotides.
Authors: Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E
Journal: Biochemistry (1997): 13223-31

Characterization of the p68/p58 heterodimer of human immunodeficiency virus type 2 reverse transcriptase.
Authors: Fan, N and Rank, K B and Poppe, S M and Tarpley, W G and Sharma, S K
Journal: Biochemistry (1996): 1911-7

Mechanism of inhibition of DNA synthesis by 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil.
Authors: Suzutani, T and Machida, H and Honess, R W
Journal: Microbiology and immunology (1993): 511-3

Expression of enzymatically active reverse transcriptase of simian immunodeficiency virus in bacteria: sensitivity to nucleotide analogue inhibitors.
Authors: Prasad, V R and Myrick, K and Haseltine, W and Goff, S P
Journal: Virology (1990): 896-900

Wheat embryo DNA polymerase A reverse transcribes natural and synthetic RNA templates. Biochemical characterization and comparison with animal DNA polymerase gamma and retroviral reverse transcriptase.
Authors: Laquel, P and Sallafranque-Andreola, M and Tarrago-Litvak, L and Castroviejo, M and Litvak, S
Journal: Biochimica et biophysica acta (1990): 139-48

3′-Deoxy-3′-fluorothymidinetriphosphate: inhibitor and terminator of DNA synthesis catalysed by DNA polymerase beta, terminal deoxynucleotidyl transferase and DNA polymerase I.
Authors: Matthes, E and Lehmann, C and Drescher, B and Büttner, W and Langen, P
Journal: Biomedica biochimica acta (1985): K63-73

A fidelity assay using “dideoxy” DNA sequencing: a measurement of sequence dependence and frequency of forming 5-bromouracil X guanine base mispairs.
Authors: Lasken, R S and Goodman, M F
Journal: Proceedings of the National Academy of Sciences of the United States of America (1985): 1301-5

说明书
ddTTP [2′,3′-Dideoxythymidine-5′-triphosphate].pdf