Cy5-dATP
|
货号 |
17038 |
存储条件 |
在零下15度以下保存, 避免光照 |
规格 |
25 nmoles |
价格 |
1200 |
Ex (nm) |
651 |
Em (nm) |
670 |
分子量 |
1182.05 |
溶剂 |
Water |
产品详细介绍 |
简要概述
产品基本信息
货号:17038
产品名称:Cy5-dATP
规格:25 nmoles
储存条件:-15℃避光防潮
保质期:24个月
产品物理化学光谱特性
分子量:1182.05
溶剂:水
激发波长(nm):651
发射波长(nm):670
消光系数(cm-1 M-1):250000
荧光量子产率:0.27(PBS),0.4(乙醇)
校正因子 (280 nm):0.03
产品介绍
染料修饰的脱氧腺苷5′-三磷酸酯广泛用于各种非放射性DNA标记反应,包括切口平移,随机引物标记,cDNA标记和3′-末端标记。 Cy5-dATP可用于通过常规酶促掺入方法(例如逆转录,缺口翻译,随机引物标记或PCR)产生含Cy5的DNA。这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。此Cy5-dATP缀合物可与SpectrumFRed 滤波片组一起用作红色荧光色(SpectrumFRed 是Vysis的商标)。它在功能上等同于Cy5-dATP(珀金·埃尔默(Perkin Elmer)的NEL593001EA),稳定性大大提高。
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参考文献
2-Formyl-dATP as Substrate for Polymerase Synthesis of Reactive DNA Bearing an Aldehyde Group in the Minor Groove.
Authors: Krömer, Matouš and Brunderová, Mária and Ivancová, Ivana and Poštová Slavětínská, Lenka and Hocek, Michal
Journal: ChemPlusChem (2020): 1164-1170
Fluorescent dATP for DNA Synthesis In Vivo.
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Journal: Molecular cell (2019): 845-856.e5
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Journal: ACS sensors (2019): 1090-1096
Terminal deoxynucleotidyl transferase-initiated molecule beacons arrayed aptamer probe for sensitive detection of metastatic colorectal cancer cells.
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Journal: Talanta (2019): 152-158
2-Allyl- and Propargylamino-dATPs for Site-Specific Enzymatic Introduction of a Single Modification in the Minor Groove of DNA.
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Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 14938-14941
DNA Primer Extension with Cyclopropenylated 7-Deaza-2′-deoxyadenosine and Efficient Bioorthogonal Labeling in Vitro and in Living Cells.
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Journal: Virus research (2018): 7-16