Monarch Plasmid Resuspension Buffer (B1) is designed for use with the Monarch Plasmid Miniprep Kit (T1010S/L). This buffer is the first one used in the miniprep workflow and is used to resuspend the cell pellet after the initial centrifugation of your cell culture. For easy identification, this buffer is colored pink.

Learn More About Monarch

• NEBMonarch.com
• Optimize your results with our unique column design
• Enhance your DNA purification experience
• Feel good about choosing Monarch
• Choose Monarch kits for pure value

Storage Temperature

25°C 

Companion Products

• Monarch™ Plasmid Miniprep Kit 
• Monarch™ Plasmid Miniprep Columns
• Monarch™ DNA Elution Buffer
• Monarch™ Plasmid Wash Buffer 2
• Monarch™ Plasmid Wash Buffer 1
• Monarch™ Plasmid Lysis Buffer (B2)
• Monarch™ Plasmid Neutralization Buffer (B3)

Monarch Plasmid Lysis Buffer (B2) is designed for use with the Monarch Plasmid Miniprep Kit (T1010S/L). The Lysis buffer is used to break open the cells under alkaline conditions in order to release the cellular components, including the plasmid DNA. For easy identification, the buffer is colored blue. This also helps to monitor the completion of the cell lysis step.

Learn More About Monarch

• NEBMonarch.com
• Optimize your results with our unique column design
• Enhance your DNA purification experience
• Feel good about choosing Monarch
• Choose Monarch kits for pure value

Storage Temperature

25°C 

Companion Products

• Monarch™ Plasmid Miniprep Kit 
• Monarch™ Plasmid Miniprep Columns
• Monarch™ DNA Elution Buffer
• Monarch™ Plasmid Wash Buffer 2
• Monarch™ Plasmid Resuspension Buffer (B1)
• Monarch™ Plasmid Wash Buffer 1
• Monarch™ Plasmid Neutralization Buffer (B3)

Monarch Plasmid Neutralization Buffer is designed for use with the Monarch Plasmid Miniprep Kit (T1010S/L). Please note that this buffer contains RNase A and should be stored at 4°C after opening. This buffer is used to neutralize the lysate and digest any RNA present. The buffer also prepares the DNA for binding to the column matrix. It is conveniently colored yellow for identification as well as for monitoring when the neutralization is complete.

Learn More About Monarch

• NEBMonarch.com
• Optimize your results with our unique column design
• Enhance your DNA purification experience
• Feel good about choosing Monarch
• Choose Monarch kits for pure value

Storage Temperature

4°C 

Companion Products

• Monarch™ Plasmid Miniprep Kit 
• Monarch™ Plasmid Miniprep Columns
• Monarch™ DNA Elution Buffer
• Monarch™ Plasmid Wash Buffer 2
• Monarch™ Plasmid Resuspension Buffer (B1)
• Monarch™ Plasmid Lysis Buffer (B2)
• Monarch™ Plasmid Wash Buffer 1

1.    Contains RNase A

Monarch Plasmid Wash Buffer 1 is designed for use with the Monarch Plasmid Miniprep Kit (T1010S/L). This is the first wash buffer used to wash the DNA and remove cellular components after the initial binding to the column.

Learn More About Monarch

• NEBMonarch.com
• Optimize your results with our unique column design
• Enhance your DNA purification experience
• Feel good about choosing Monarch
• Choose Monarch kits for pure value

Storage Temperature

25°C 

Companion Products

• Monarch™ Plasmid Miniprep Kit 
• Monarch™ Plasmid Miniprep Columns
• Monarch™ DNA Elution Buffer
• Monarch™ Plasmid Wash Buffer 2
• Monarch™ Plasmid Resuspension Buffer (B1)
• Monarch™ Plasmid Lysis Buffer (B2)
• Monarch™ Plasmid Neutralization Buffer (B3)

Monarch Plasmid Wash Buffer 2 is designed for use with the Monarch Plasmid Miniprep Kit (T1010S/L). This is the second wash buffer used to wash the DNA and remove trace buffer components, including salts.

Learn More About Monarch

• NEBMonarch.com
• Optimize your results with our unique column design
• Enhance your DNA purification experience
• Feel good about choosing Monarch
• Choose Monarch kits for pure value

Storage Temperature

25°C 

Companion Products

• Monarch™ Plasmid Miniprep Kit 
• Monarch™ Plasmid Miniprep Columns
• Monarch™ DNA Elution Buffer
• Monarch™ Plasmid Wash Buffer 1
• Monarch™ Plasmid Resuspension Buffer (B1)
• Monarch™ Plasmid Lysis Buffer (B2)
• Monarch™ Plasmid Neutralization Buffer (B3)

Monarch Plasmid Miniprep Columns, supplied with the Monarch Plasmid Miniprep Kit, have been custom designed to deliver excellent performance for your plasmid purification. Monarch Columns are designed without a frit (which is commonly used in purification columns to hold the membrane in place), eliminating buffer retention and the risk of carry-over contamination. The tapered design of the Miniprep Column enables elution in as little as 30 μl. Monarch columns use less plastic than conventional columns, reducing their environmental footprint without compromising performance. The columns fit snugly into the collection tubes to enable easy handling. The Monarch Miniprep Columns also feature a convenient tab so that you can label your columns easily without removing them from your tube rack. Monarch columns are suitable for use with centrifugation and vacuum purification protocols. 

Monarch Plasmid Miniprep Column Design

Learn More About Monarch

• NEBMonarch.com
• Optimize your results with our unique column design
• Enhance your DNA purification experience
• Feel good about choosing Monarch
• Choose Monarch kits for pure value

Highlights

• No buffer retention/risk of carryover contamination
• Low elution volume (≥ 30 μl)
• Convenient tab for easy labeling
• Snug fit into collection tubes for easy handling
• Made with less plastic than conventional columns

Storage Temperature

25°C

Binding Capacity

20 µg