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Screen Quest CHO-Gqi嵌合体细胞系
| 货号 | 38101 | 存储条件 | 在零下80度以下保存 |
| 规格 | Each | 价格 | 0 |
| Ex (nm) | Em (nm) | ||
| 分子量 | 溶剂 | ||
| 产品详细介绍 | |||
简要概述
产品基本信息
货号：38101
产品名称：Screen Quest CHO-Gqi嵌合体细胞系
规格：Each
适用仪器
| 其他仪器 |
| FDSS, FLIPR, ViewLux, NOVOStar, ArrayScan, FlexStation, IN Cell Analyzer |
产品介绍
Screen Quest 细胞系是一系列细胞，已成功用于药物发现和筛选环境中，用于研究通常不通过细胞内钙偶联的G蛋白偶联受体（GPCR）。它已与FLIPR，FDSS系统有效结合，与许多受体的非Gq偶联成员结合使用，例如趋化因子，5-羟色胺，谷氨酸，多巴胺，阿片样物质，血管加压素以及α和β-肾上腺素能受体家族。超过60％的已知G蛋白偶联受体通过Gq以外的途径发出信号，从而导致细胞内钙增加，并且随着基因组学揭示出更多的GPCR靶标，这种趋势持续增加。Screen Quest 细胞系用于研究通常不通过细胞内钙偶联的GPCR。Screen Quest 细胞系基于一系列G蛋白嵌合体，包括混杂的G蛋白Gα16。嵌合体由Gq蛋白复合物的α亚基组成，该复合物的5个羧基末端氨基酸已被其他G蛋白之一（Gαs，Gαi，Gαo或Gαz）取代。这些氨基酸负责受体与其G蛋白的偶联。这些嵌合体与通常通过cAMP途径起作用的特定非Gq偶联受体的共表达可能会导致受体刺激后细胞内钙信号的产生。Screen Quest CHO-Gqi细胞系是被嵌合Gqi alpha亚基蛋白稳定转染的CHO-K1细胞。当用作转染表达Gi偶联受体的宿主细胞时，细胞中组成型表达的Gqi蛋白可使通常通过cAMP途径起作用的转染受体与Gq信号转导和动员的细胞内钙偶联。可以使用钙敏感染料（例如Calbryte 520 AM，Cal-520 AM，Fluo-8 AM或Fluo-4 AM）和无需洗涤的钙试剂盒检测特定配体对这些非Gq偶联受体的激活。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Screen Quest CHO-Gqi嵌合体细胞系。
图示
 图1.用Cal-520®，AM（Cat＃21130）在CHO-Ga16-NOP细胞中测量了伤害感受肽刺激的钙反应。将CHO-Ga16-NOP细胞以60,000个细胞/ 100 µL /孔在Costar黑色96孔板中过夜接种。在含有20 mM Hepes缓冲液（HHBS）的Hanks中，将等体积（100μL）的10μMCal-520®AM和2 mM丙磺舒在37°C下孵育1小时。用具有1 mM丙磺舒的HHBS代替Cal-520®AM上样溶液。通过FlexStation（分子设备）添加了Nociceptin，以达到最终指示的浓度。 |
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