鬼笔环肽-赖氨酸标记

	货号	5304	存储条件	在零下15度以下保存, 避免光照
	规格	100 ug	价格	1164
	Ex (nm)		Em (nm)
	分子量	885.91	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：5304

产品名称：鬼笔环肽-赖氨酸标记

规格：100ug

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：885.91

溶剂：DMSO

产品介绍

鬼笔环肽赖氨酸是一种方便的化合物，可用于开发各种鬼笔环肽衍生物，这些衍生物可用于研究细胞结构和其他生物学应用。它易于与胺反应性染料，生物素和其他标签分子（例如NHS酯，异硫氰酸酯和磺酰氯等）反应。鬼笔环肽是一种双环七肽毒素，特异性结合在F-肌动蛋白亚基之间的界面上，将相邻的亚基锁定在一起。鬼笔环肽与肌动蛋白丝的结合比与肌动蛋白单体的结合要紧密得多，从而导致肌动蛋白亚基从丝端解离的速率常数降低，从而通过防止丝解聚而基本稳定了肌动蛋白丝。鬼笔环肽的性质是研究F-肌动蛋白在细胞中分布的有用工具，方法是用荧光类似物标记鬼笔环肽并将其用于染色肌动蛋白丝以进行光学显微镜检查。已经证明，鬼笔环肽的荧光衍生物在定位活细胞或固定细胞中的肌动蛋白丝以及在体外可视化单个肌动蛋白丝方面非常有用。荧光鬼笔环肽衍生物已被用作高分辨率研究肌动蛋白网络的重要工具。 AAT Bioquest为多色成像应用提供了多种具有不同颜色的荧光鬼笔环肽衍生物。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的鬼笔环肽-赖氨酸标记。 

参考文献

Hybrid elastomer-plastic microfluidic device as a convenient model for mimicking the blood-brain barrier in vitro.
Authors: Nguyen, Phuoc Quang Huy and Duong, Duong Duy and Kwun, Jun Dae and Lee, Nae Yoon
Journal: Biomedical microdevices (2019): 90

Purinergic Signaling and Aminoglycoside Ototoxicity: The Opposing Roles of P1 (Adenosine) and P2 (ATP) Receptors on Cochlear Hair Cell Survival.
Authors: Lin, Shelly C Y and Thorne, Peter R and Housley, Gary D and Vlajkovic, Srdjan M
Journal: Frontiers in cellular neuroscience (2019): 207

Gross anatomy of the muscle systems and associated innervation of Apatemon cobitidis proterorhini metacercaria (Trematoda: Strigeidea), as visualized by confocal microscopy.
Authors: Stewart, M T and Mousley, A and Koubková, B and Sebelová, S and Marks, N J and Halton, D W
Journal: Parasitology (2003): 273-82

Light and electron microscopic study of changes in the organization of the cortical actin cytoskeleton during chromaffin cell secretion.
Authors: Tchakarov, L E and Zhang, L and Rosé, S D and Tang, R and Trifaró, J M
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1998): 193-203

Responses induced by tacrine in neuronal and non-neuronal cell lines.
Authors: De Ferrari, G V and von Bernhardi, R and Calderón, F H and Luza, S C and Inestrosa, N C
Journal: Journal of neuroscience research (1998): 435-44

Expression of PAC 1, an epitope associated with two synapse-enriched glycoproteins and a neuronal cytoskeleton-associated polypeptide in developing forebrain neurons.
Authors: Willmott, T and Williamson, T L and Mummery, R and Hawkes, R B and Can, A and Gurd, J W and Gordon-Weeks, P R and Beesley, P W
Journal: Neuroscience (1994): 115-29

Vasoactive amines directly modify endothelial cells to affect polymorphonuclear leukocyte diapedesis in vitro.
Authors: Doukas, J and Shepro, D and Hechtman, H B
Journal: Blood (1987): 1563-9

鬼笔环肽-赖氨酸标记

	货号	5305	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	7956
	Ex (nm)		Em (nm)
	分子量	885.91	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：5305

产品名称：鬼笔环肽-赖氨酸标记

规格：1mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：885.91

溶剂：DMSO

产品介绍

鬼笔环肽赖氨酸是一种方便的化合物，可用于开发各种鬼笔环肽衍生物，这些衍生物可用于研究细胞结构和其他生物学应用。它易于与胺反应性染料，生物素和其他标签分子（例如NHS酯，异硫氰酸酯和磺酰氯等）反应。鬼笔环肽是一种双环七肽毒素，特异性结合在F-肌动蛋白亚基之间的界面上，将相邻的亚基锁定在一起。鬼笔环肽与肌动蛋白丝的结合比与肌动蛋白单体的结合要紧密得多，从而导致肌动蛋白亚基从丝端解离的速率常数降低，从而通过防止丝解聚而基本稳定了肌动蛋白丝。鬼笔环肽的性质是研究F-肌动蛋白在细胞中分布的有用工具，方法是用荧光类似物标记鬼笔环肽并将其用于染色肌动蛋白丝以进行光学显微镜检查。已经证明，鬼笔环肽的荧光衍生物在定位活细胞或固定细胞中的肌动蛋白丝以及在体外可视化单个肌动蛋白丝方面非常有用。荧光鬼笔环肽衍生物已被用作高分辨率研究肌动蛋白网络的重要工具。 AAT Bioquest为多色成像应用提供了多种具有不同颜色的荧光鬼笔环肽衍生物。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的鬼笔环肽-赖氨酸标记。 

参考文献

Hybrid elastomer-plastic microfluidic device as a convenient model for mimicking the blood-brain barrier in vitro.
Authors: Nguyen, Phuoc Quang Huy and Duong, Duong Duy and Kwun, Jun Dae and Lee, Nae Yoon
Journal: Biomedical microdevices (2019): 90

Purinergic Signaling and Aminoglycoside Ototoxicity: The Opposing Roles of P1 (Adenosine) and P2 (ATP) Receptors on Cochlear Hair Cell Survival.
Authors: Lin, Shelly C Y and Thorne, Peter R and Housley, Gary D and Vlajkovic, Srdjan M
Journal: Frontiers in cellular neuroscience (2019): 207

Gross anatomy of the muscle systems and associated innervation of Apatemon cobitidis proterorhini metacercaria (Trematoda: Strigeidea), as visualized by confocal microscopy.
Authors: Stewart, M T and Mousley, A and Koubková, B and Sebelová, S and Marks, N J and Halton, D W
Journal: Parasitology (2003): 273-82

Light and electron microscopic study of changes in the organization of the cortical actin cytoskeleton during chromaffin cell secretion.
Authors: Tchakarov, L E and Zhang, L and Rosé, S D and Tang, R and Trifaró, J M
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1998): 193-203

Responses induced by tacrine in neuronal and non-neuronal cell lines.
Authors: De Ferrari, G V and von Bernhardi, R and Calderón, F H and Luza, S C and Inestrosa, N C
Journal: Journal of neuroscience research (1998): 435-44

Expression of PAC 1, an epitope associated with two synapse-enriched glycoproteins and a neuronal cytoskeleton-associated polypeptide in developing forebrain neurons.
Authors: Willmott, T and Williamson, T L and Mummery, R and Hawkes, R B and Can, A and Gurd, J W and Gordon-Weeks, P R and Beesley, P W
Journal: Neuroscience (1994): 115-29

Vasoactive amines directly modify endothelial cells to affect polymorphonuclear leukocyte diapedesis in vitro.
Authors: Doukas, J and Shepro, D and Hechtman, H B
Journal: Blood (1987): 1563-9

DNP-PEG4 NHS酯 CAS 858126-78-0

	货号	2023	存储条件	在零下15度以下保存, 避免光照
	规格	5 mg	价格	1164
	Ex (nm)		Em (nm)
	分子量	528.47	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：2023

产品名称：DNP-PEG4琥珀酰亚胺酯

CAS：858126-78-0

规格：5mg

储存条件：-15℃，避光干燥

保质期：12个月

物理化学光谱特性

分子量：528.47

溶剂：DMSO

产品介绍

与常用的DNP-X SE（＃2021）相比，DNP-PEG4酸具有更好的水溶性。它是可被抗DNP抗体识别的探针的胺反应性构建基块。它也可以用作与Trp或Tyr配对的出色的胺反应性FRET淬灭剂。它的末端NHS酯基很容易与伯胺反应形成稳定的酰胺键。亲水性PEG接头的增加使得与生物分子缀合的DNP基团的数目增加，而不会引起沉淀。

参考文献

Nano-Snowflower of Gold Nanoparticles-Ruthenium Metallopolymer-Carbon Nanotubes Binding Anti-DNP IgE Antibody.
Authors: Li, Huayang and Wu, Jie and Melnyczuk, John M and Olubi, Omotunde and Lewis, Lauchon I and Cao, Yang and Nagappan, Peri and Khan, Shafiq A and Ingram, Conrad W and Harruna, Issifu I
Journal: Journal of nanoscience and nanotechnology (2015): 5733-40

Peptide mimics of hapten DNP: the effect of affinity of anti-DNP monoclonal antibodies for the selection of phage-displayed mimotopes.
Authors: Kalaycioglu, Atila T and Russell, Peter H and Howard, Colin R
Journal: Journal of immunological methods (2011): 36-42

Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers.
Authors: Sehgal, D and Schiaffella, E and Anderson, A O and Mage, R G
Journal: European journal of immunology (2000): 3634-44

Determination of the binding of ligands containing the N-2,4-dinitrophenyl group to bivalent monoclonal rat anti-DNP antibody using affinity capillary electrophoresis.
Authors: Mammen, M and Gomez, F A and Whitesides, G M
Journal: Analytical chemistry (1995): 3526-35

Fast solid support detection of human papillomavirus in in vitro amplified DNA using a DNP-anti DNP monoclonal antibody couple.
Authors: Baccard-Longère, M and Alpha-Bazin, B and Chypre, C and Sauvaigo, S and Téoule, R and Bernard, P and Seigneurin, J M
Journal: Journal of virological methods (1994): 29-38

[Effects of Celosia argentea and Cucurbita moschata extracts on anti-DNP IgE antibody production in mice].
Authors: Imaoka, K and Ushijima, H and Inouye, S and Takahashi, T and Kojima, Y
Journal: Arerugi = [Allergy] (1994): 652-9

[Effects of Perilla frutescens extract on anti-DNP IgE antibody production in mice].
Authors: Imaoka, K and Inouye, S and Takahashi, T and Kojima, Y
Journal: Arerugi = [Allergy] (1993): 74-80

Effect of anti-DNP IgG1- and IgG2a-secreting hybridomas in vivo on the development of an anti-DNP IgE antibody response in mice.
Authors: Hagen, M and Morrison, B and Robbinson, D and Strejan, G H
Journal: International archives of allergy and immunology (1992): 146-53

Mechanism of allergic cross-reactions–I. Multispecific binding of ligands to a mouse monoclonal anti-DNP IgE antibody.
Authors: Varga, J M and Kalchschmid, G and Klein, G F and Fritsch, P
Journal: Molecular immunology (1991): 641-54

Mechanism of allergic cross-reactions–II. Cross-stimulation, by chemically unrelated ligands, of rat basophilic leukemia cells sensitized with an anti-DNP IgE antibody.
Authors: Varga, J M and Kalchschmid, G and Klein, G F and Fritsch, P
Journal: Molecular immunology (1991): 655-9

Cell Meter Beta-Arrestin蛋白质易位GPCR信号检测试剂盒

	货号	36390	存储条件	在零下15度以下保存, 避免光照
	规格	100 Tests	价格	9180
	Ex (nm)		Em (nm)
	分子量		溶剂
	产品详细介绍

简要概述

所有G蛋白偶联受体（GPCR）在通过配体结合激活后，都会通过一条共同途径迅速发生脱敏作用。 β-arrestin（一种细胞质蛋白）与激活的受体的结合使GPCR信号失活，并启动了受体向细胞内的易位，在此细胞中配体被去除，受体被循环回到细胞膜。通过将荧光标记（例如GFP）附着到β-arrestin，可以检测受体arrestin复合物的位置。由于脱敏仅发生在激活的受体上，因此检测β-arrestin的易位和随后的受体再循环提供了检测GPCR靶标激活的可靠方法。 Cell Meter Beta-Arrestin蛋白易位GPCR信号试剂盒提供了功能强大的功能分析，可通过荧光成像筛选目标化合物针对已知或孤立GPCR靶标的活性。靶向GPCR的激活诱导荧光向细胞膜和内吞囊泡的移位。

产品说明书

样品实验方案

概述

准备细胞进行转染
准备Transfectamine 5000-DNA混合物
将Transfectamine 5000-DNA混合物添加到细胞培养物中，并孵育过夜
转染后24-30小时将转染的细胞转移到96孔板中，并将培养物孵育过夜
在荧光显微镜下分析由GPCR激活引起的转运

细胞准备

细胞密度在转染时它们将达到约60-70％的融合度。
转染前用新鲜的生长培养基替换。
注意：例如，对于6孔板，每孔替换为2 mL培养基，对于10 cm板，则替换为6 mL培养基。

储备溶液配制

除非另有说明，否则所有未使用的储备溶液应分为一次性使用的等分试样，并在制备后储存在-20°C下。避免重复冻融循环。

β-arrestin-GFPDNA储备溶液
向小瓶β-arrestin-GFPDNA（组分A）中加入10 µL ddH2O，充分混合至终浓度为1 µg / µL。

操作步骤

1.将3 µg DNA（例如1.5 µg Beta-arrestin-GFP DNA储备液和1.5 µg您准备的GPCR DNA）与200 µL无血清培养基混合。
2.向混合物中加入9 µL Transfectamine 5000（组分B）。
3.充分混合并在室温下孵育20分钟。
注意：Transfectamine 5000和DNA的比例需要针对不同的细胞系进行优化，通常，在我们的测试中，Transfectamine 5000转染试剂（µL）与DNA（µg）的比例应为3-5 µL：1ug。

表1. 6孔板和10 cm板的样本表

转染和转运方案
1.将Transfectamine 5000 -DNA混合物添加到培养板中，并孵育过夜。
注意事项：最早在转染后16小时即可检测到重组蛋白。转染后72〜96小时可观察到最大表达水平。
2.转染后24-30小时将转染的细胞转移到96孔板中，并孵育过夜。
使用FITC滤光片（Ex / Em = 488/530 nm）在荧光显微镜下检测受体激活诱导的β-arrestin易位。

图示

图1. HeLa细胞中β-arrestin的易位。 HeLa细胞用β-arrestin-GFP和加压素受体2（V2R）瞬时转染。 HeLa细胞在6孔板中培养，并融合至〜60％。用9 µL Transfectamine 5000转染等量的β-arrestin-GFP（1.5 µg）和V2R质粒（1.5 µg）。转染后约30小时，将细胞转移到96孔板中。转染后约48小时，将加压素（1 µM）加入细胞中以诱导β-arrestin-GFP易位。使用FITC通道在荧光显微镜下加压素处理之前和之后2小时拍摄图像。

参考文献

Screening cellular feature measurements for image-based assay development.
Authors: Logan, David J and Carpenter, Anne E
Journal: Journal of biomolecular screening (2010): 840-6

Kappa opioid receptor screen with the Tango beta-arrestin recruitment technology and characterization of hits with second-messenger assays.
Authors: Doucette, Christopher and Vedvik, Kevin and Koepnick, Elizabeth and Bergsma, Aaron and Thomson, Brian and Turek-Etienne, Tammy C
Journal: Journal of biomolecular screening (2009): 381-94

Multiplexed assays by high-content imaging for assessment of GPCR activity.
Authors: Ross, D A and Lee, S and Reiser, V and Xue, J and Alves, K and Vaidya, S and Kreamer, A and Mull, R and Hudak, E and Hare, T and Detmers, P A and Lingham, R and Ferrer, M and Strulovici, B and Santini, F
Journal: Journal of biomolecular screening (2008): 449-55

Comparison of G-protein coupled receptor desensitization-related beta-arrestin redistribution using confocal and non-confocal imaging.
Authors: Haasen, Dorothea and Wolff, Michael and Valler, Martin J and Heilker, Ralf
Journal: Combinatorial chemistry & high throughput screening (2006): 37-47

High-content screening of known G protein-coupled receptors by arrestin translocation.
Authors: Hudson, Christine C and Oakley, Robert H and Sjaastad, Michael D and Loomis, Carson R
Journal: Methods in enzymology (2006): 63-78

High-throughput confocal microscopy for beta-arrestin-green fluorescent protein translocation G protein-coupled receptor assays using the Evotec Opera.
Authors: Garippa, Ralph J and Hoffman, Ann F and Gradl, Gabriele and Kirsch, Achim
Journal: Methods in enzymology (2006): 99-120

The ligand-independent translocation assay: an enabling technology for screening orphan G protein-coupled receptors by arrestin recruitment.
Authors: Oakley, Robert H and Hudson, Christine C and Sjaastad, Michael D and Loomis, Carson R
Journal: Methods in enzymology (2006): 50-63

Development and validation of algorithms for measuring G-protein coupled receptor activation in cells using the LSC-based imaging cytometer platform.
Authors: Ozawa, Kazuo and Hudson, Christine C and Wille, Kirsten R and Karaki, Sachiko and Oakley, Robert H
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 69-76

Quantitative cell-based high-content screening for vasopressin receptor agonists using transfluor technology.
Authors: Ghosh, Richik N and DeBiasio, Richard and Hudson, Christine C and Ramer, Everett R and Cowan, Conrad L and Oakley, Robert H
Journal: Journal of biomolecular screening (2005): 476-84

The cellular distribution of fluorescently labeled arrestins provides a robust, sensitive, and universal assay for screening G protein-coupled receptors.
Authors: Oakley, Robert H and Hudson, Christine C and Cruickshank, Rachael D and Meyers, Diane M and Payne, Richard E and Rhem, Shay M and Loomis, Carson R
Journal: Assay and drug development technologies (2002): 21-30

Cell Meter Beta-Arrestin蛋白质易位GPCR信号检测试剂盒

	货号	36391	存储条件	在零下15度以下保存, 避免光照
	规格	200 Tests	价格	11628
	Ex (nm)		Em (nm)
	分子量		溶剂
	产品详细介绍

简要概述

所有G蛋白偶联受体（GPCR）在通过配体结合激活后，都会通过一条共同途径迅速发生脱敏作用。 β-arrestin（一种细胞质蛋白）与激活的受体的结合使GPCR信号失活，并启动了受体向细胞内的易位，在此细胞中配体被去除，受体被循环回到细胞膜。通过将荧光标记（例如GFP）附着到β-arrestin，可以检测受体arrestin复合物的位置。由于脱敏仅发生在激活的受体上，因此检测β-arrestin的转运和随后的受体再循环提供了检测GPCR靶标激活的可靠方法。 Cell Meter Beta-Arrestin蛋白易位GPCR信号试剂盒提供了功能强大的功能分析，可通过荧光成像筛选目标化合物针对已知或孤立GPCR靶标的活性。靶向GPCR的激活诱导荧光向细胞膜和内吞囊泡的移位。

产品说明书

样品实验方案

概述

准备细胞进行转染
准备Transfectamine 5000-DNA混合物
将Transfectamine 5000-DNA混合物添加到细胞培养物中，并孵育过夜
转染后24-30小时将转染的细胞转移到96孔板中，并将培养物孵育过夜
在荧光显微镜下分析由GPCR激活引起的转运

细胞准备

细胞密度在转染时它们将达到约60-70％的融合度。
转染前用新鲜的生长培养基替换。
注意：例如，对于6孔板，每孔替换为2 mL培养基，对于10 cm板，则替换为6 mL培养基。

储备溶液配制

除非另有说明，否则所有未使用的储备溶液应分为一次性使用的等分试样，并在制备后储存在-20°C下。避免重复冻融循环。

β-arrestin-GFPDNA储备溶液
向小瓶β-arrestin-GFPDNA（组分A）中加入10 µL ddH2O，充分混合至终浓度为1 µg / µL。

操作步骤

1.将3 µg DNA（例如1.5 µg Beta-arrestin-GFP DNA储备液和1.5 µg您准备的GPCR DNA）与200 µL无血清培养基混合。
2.向混合物中加入9 µL Transfectamine 5000（组分B）。
3.充分混合并在室温下孵育20分钟。
注意：Transfectamine 5000和DNA的比例需要针对不同的细胞系进行优化，通常，在我们的测试中，Transfectamine 5000转染试剂（µL）与DNA（µg）的比例应为3-5 µL：1ug。

表1. 6孔板和10 cm板的样本表

转染和转运方案
1.将Transfectamine 5000 -DNA混合物添加到培养板中，并孵育过夜。
注意事项：最早在转染后16小时即可检测到重组蛋白。转染后72〜96小时可观察到最大表达水平。
2.转染后24-30小时将转染的细胞转移到96孔板中，并孵育过夜。
使用FITC滤光片（Ex / Em = 488/530 nm）在荧光显微镜下检测受体激活诱导的β-arrestin易位。

图示

图1. HeLa细胞中β-arrestin的易位。 HeLa细胞用β-arrestin-GFP和加压素受体2（V2R）瞬时转染。 HeLa细胞在6孔板中培养，并融合至〜60％。用9 µL Transfectamine 5000转染等量的β-arrestin-GFP（1.5 µg）和V2R质粒（1.5 µg）。转染后约30小时，将细胞转移到96孔板中。转染后约48小时，将加压素（1 µM）加入细胞中以诱导β-arrestin-GFP易位。使用FITC通道在荧光显微镜下加压素处理之前和之后2小时拍摄图像。

参考文献

Screening cellular feature measurements for image-based assay development.
Authors: Logan, David J and Carpenter, Anne E
Journal: Journal of biomolecular screening (2010): 840-6

Kappa opioid receptor screen with the Tango beta-arrestin recruitment technology and characterization of hits with second-messenger assays.
Authors: Doucette, Christopher and Vedvik, Kevin and Koepnick, Elizabeth and Bergsma, Aaron and Thomson, Brian and Turek-Etienne, Tammy C
Journal: Journal of biomolecular screening (2009): 381-94

Multiplexed assays by high-content imaging for assessment of GPCR activity.
Authors: Ross, D A and Lee, S and Reiser, V and Xue, J and Alves, K and Vaidya, S and Kreamer, A and Mull, R and Hudak, E and Hare, T and Detmers, P A and Lingham, R and Ferrer, M and Strulovici, B and Santini, F
Journal: Journal of biomolecular screening (2008): 449-55

Comparison of G-protein coupled receptor desensitization-related beta-arrestin redistribution using confocal and non-confocal imaging.
Authors: Haasen, Dorothea and Wolff, Michael and Valler, Martin J and Heilker, Ralf
Journal: Combinatorial chemistry & high throughput screening (2006): 37-47

High-content screening of known G protein-coupled receptors by arrestin translocation.
Authors: Hudson, Christine C and Oakley, Robert H and Sjaastad, Michael D and Loomis, Carson R
Journal: Methods in enzymology (2006): 63-78

High-throughput confocal microscopy for beta-arrestin-green fluorescent protein translocation G protein-coupled receptor assays using the Evotec Opera.
Authors: Garippa, Ralph J and Hoffman, Ann F and Gradl, Gabriele and Kirsch, Achim
Journal: Methods in enzymology (2006): 99-120

The ligand-independent translocation assay: an enabling technology for screening orphan G protein-coupled receptors by arrestin recruitment.
Authors: Oakley, Robert H and Hudson, Christine C and Sjaastad, Michael D and Loomis, Carson R
Journal: Methods in enzymology (2006): 50-63

Development and validation of algorithms for measuring G-protein coupled receptor activation in cells using the LSC-based imaging cytometer platform.
Authors: Ozawa, Kazuo and Hudson, Christine C and Wille, Kirsten R and Karaki, Sachiko and Oakley, Robert H
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 69-76

Quantitative cell-based high-content screening for vasopressin receptor agonists using transfluor technology.
Authors: Ghosh, Richik N and DeBiasio, Richard and Hudson, Christine C and Ramer, Everett R and Cowan, Conrad L and Oakley, Robert H
Journal: Journal of biomolecular screening (2005): 476-84

The cellular distribution of fluorescently labeled arrestins provides a robust, sensitive, and universal assay for screening G protein-coupled receptors.
Authors: Oakley, Robert H and Hudson, Christine C and Cruickshank, Rachael D and Meyers, Diane M and Payne, Richard E and Rhem, Shay M and Loomis, Carson R
Journal: Assay and drug development technologies (2002): 21-30

FAM-xtra 亚磷酰胺

	货号	6037	存储条件	在零下15度以下保存, 避免光照
	规格	50 umoles	价格	1164
	Ex (nm)	493	Em (nm)	517
	分子量	879.94	溶剂	MeCN
	产品详细介绍

简要概述

产品基本信息

货号：6037

产品名称：FAM-xtra 亚磷酰胺

规格：50 umoles

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：879.94

溶剂：MeCN

产品介绍

6-FAM亚磷酰胺可能是最流行的荧光构建基块，已广泛用于通过荧光素荧光团有效标记5’端的寡核苷酸。标准裂解和氢氧化铵脱保护用于释放荧光素标记的寡核苷酸。尽管6-FAM已普遍用于开发各种qPCR探针，但其荧光受到两个作用限制：1.高度依赖pH值；2.光漂白迅速。目前我们已开发出FAM-xtra 亚磷酰胺解决了这两个局限性，同时完整保留了6-FAM亚磷酰胺的所有优点。 FAM-xtra亚磷酰胺可在相同操作条件下与6-FAM亚磷酰胺完全一样​​使用，而无需进行任何更改。由FAM-xtra亚磷酰胺制成的寡核苷酸具有许多优点：1.FAM-xtra标记的寡核苷酸具有与6-FAM标记的寡核苷酸相同的光谱。 2.FAM-xtra标记的寡核苷酸比相应的FAM-标记的寡核苷酸明亮得多。 3.FAM-xtra标记的寡核苷酸比相应的FAM-标记的寡核苷酸具有更高的光稳定性。4.与相应的FAM标记的寡核苷酸相比，FAM-xtra标记的寡核苷酸的荧光对pH的敏感度低得多。在生理条件下，FAM-xtra标记的寡核苷酸的荧光基本上与pH无关，这使得FAM-xtra标记的寡核苷酸的qPCR探针更加稳定。

参考文献

Development of an Innovative Intradermal siRNA Delivery System Using a Combination of a Functional Stearylated Cytoplasm-Responsive Peptide and a Tight Junction-Opening Peptide.
Authors: Ibaraki, Hisako and Kanazawa, Takanori and Takashima, Yuuki and Okada, Hiroaki and Seta, Yasuo
Journal: Molecules (Basel, Switzerland) (2016)

Non-nucleoside phosphoramidites of xanthene dyes (FAM, JOE, and TAMRA) for oligonucleotide labeling.
Authors: Kvach, Maksim V and Tsybulsky, Dmitry A and Shmanai, Vadim V and Prokhorenko, Igor A and Stepanova, Irina A and Korshun, Vladimir A
Journal: Current protocols in nucleic acid chemistry (2013): Unit 4.55

Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes.
Authors: Kvach, Maksim V and Stepanova, Irina A and Prokhorenko, Igor A and Stupak, Aleksander P and Bolibrukh, Dmitry A and Korshun, Vladimir A and Shmanai, Vadim V
Journal: Bioconjugate chemistry (2009): 1673-82

Synthesis and cellular uptake of fluorescently labeled multivalent hyaluronan disaccharide conjugates of oligonucleotide phosphorothioates.
Authors: Karskela, Marika and Virta, Pasi and Malinen, Melina and Urtti, Arto and Lönnberg, Harri
Journal: Bioconjugate chemistry (2008): 2549-58

Synthesis of HyBeacons and dual-labelled probes containing 2′-fluorescent groups for use in genetic analysis.
Authors: Dobson, Neil and McDowell, David G and French, David J and Brown, Lynda J and Mellor, John M and Brown, Tom
Journal: Chemical communications (Cambridge, England) (2003): 1234-5

Amplified fragment length polymorphism (AFLP) protocol for genotyping the malarial parasite Plasmodium falciparum.
Authors: Rubio, J M and Berzosa, P J and Benito, A
Journal: Parasitology (2001): 331-6

Recovery of an oligonucleotide using silver ions immobilized onto paramagnetic particles.
Authors: Ramírez-Vick, J E and García, A A and Lee, J
Journal: Preparative biochemistry & biotechnology (1998): 243-60

Fluorescent dye phosphoramidite labelling of oligonucleotides.
Authors: Theisen, P and McCollum, C and Andrus, A
Journal: Nucleic acids symposium series (1992): 99-100

FAM-xtra 亚磷酰胺

	货号	6038	存储条件	在零下15度以下保存, 避免光照
	规格	100 umoles	价格	1776
	Ex (nm)	493	Em (nm)	517
	分子量	879.94	溶剂	MeCN
	产品详细介绍

简要概述

产品基本信息

货号：6038

产品名称：FAM-xtra 亚磷酰胺

规格：100 umoles

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：879.94

溶剂：MeCN

产品介绍

6-FAM亚磷酰胺可能是最流行的荧光构建基块，已广泛用于通过荧光素荧光团有效标记5’端的寡核苷酸。标准裂解和氢氧化铵脱保护用于释放荧光素标记的寡核苷酸。尽管6-FAM已普遍用于开发各种qPCR探针，但其荧光受到两个作用限制：1.高度依赖pH值；2.光漂白迅速。目前我们已开发出FAM-xtra 亚磷酰胺解决了这两个局限性，同时完整保留了6-FAM亚磷酰胺的所有优点。 FAM-xtra亚磷酰胺可在相同操作条件下与6-FAM亚磷酰胺完全一样​​使用，而无需进行任何更改。由FAM-xtra亚磷酰胺制成的寡核苷酸具有许多优点：1.FAM-xtra标记的寡核苷酸具有与6-FAM标记的寡核苷酸相同的光谱。 2.FAM-xtra标记的寡核苷酸比相应的FAM-标记的寡核苷酸明亮得多。 3.FAM-xtra标记的寡核苷酸比相应的FAM-标记的寡核苷酸具有更高的光稳定性。4.与相应的FAM标记的寡核苷酸相比，FAM-xtra标记的寡核苷酸的荧光对pH的敏感度低得多。在生理条件下，FAM-xtra标记的寡核苷酸的荧光基本上与pH无关，这使得FAM-xtra标记的寡核苷酸的qPCR探针更加稳定。

参考文献

Development of an Innovative Intradermal siRNA Delivery System Using a Combination of a Functional Stearylated Cytoplasm-Responsive Peptide and a Tight Junction-Opening Peptide.
Authors: Ibaraki, Hisako and Kanazawa, Takanori and Takashima, Yuuki and Okada, Hiroaki and Seta, Yasuo
Journal: Molecules (Basel, Switzerland) (2016)

Non-nucleoside phosphoramidites of xanthene dyes (FAM, JOE, and TAMRA) for oligonucleotide labeling.
Authors: Kvach, Maksim V and Tsybulsky, Dmitry A and Shmanai, Vadim V and Prokhorenko, Igor A and Stepanova, Irina A and Korshun, Vladimir A
Journal: Current protocols in nucleic acid chemistry (2013): Unit 4.55

Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes.
Authors: Kvach, Maksim V and Stepanova, Irina A and Prokhorenko, Igor A and Stupak, Aleksander P and Bolibrukh, Dmitry A and Korshun, Vladimir A and Shmanai, Vadim V
Journal: Bioconjugate chemistry (2009): 1673-82

Synthesis and cellular uptake of fluorescently labeled multivalent hyaluronan disaccharide conjugates of oligonucleotide phosphorothioates.
Authors: Karskela, Marika and Virta, Pasi and Malinen, Melina and Urtti, Arto and Lönnberg, Harri
Journal: Bioconjugate chemistry (2008): 2549-58

Synthesis of HyBeacons and dual-labelled probes containing 2′-fluorescent groups for use in genetic analysis.
Authors: Dobson, Neil and McDowell, David G and French, David J and Brown, Lynda J and Mellor, John M and Brown, Tom
Journal: Chemical communications (Cambridge, England) (2003): 1234-5

Amplified fragment length polymorphism (AFLP) protocol for genotyping the malarial parasite Plasmodium falciparum.
Authors: Rubio, J M and Berzosa, P J and Benito, A
Journal: Parasitology (2001): 331-6

Recovery of an oligonucleotide using silver ions immobilized onto paramagnetic particles.
Authors: Ramírez-Vick, J E and García, A A and Lee, J
Journal: Preparative biochemistry & biotechnology (1998): 243-60

Fluorescent dye phosphoramidite labelling of oligonucleotides.
Authors: Theisen, P and McCollum, C and Andrus, A
Journal: Nucleic acids symposium series (1992): 99-100

FAM-xtra 亚磷酰胺

	货号	6039	存储条件	在零下15度以下保存, 避免光照
	规格	10×100 umoles	价格	17748
	Ex (nm)	493	Em (nm)	517
	分子量	879.94	溶剂	MeCN
	产品详细介绍

简要概述

产品基本信息

货号：6039

产品名称：FAM-xtra 亚磷酰胺

规格：10×100 umoles

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：879.94

溶剂：MeCN

产品介绍

6-FAM亚磷酰胺可能是最流行的荧光构建基块，已广泛用于通过荧光素荧光团有效标记5’端的寡核苷酸。标准裂解和氢氧化铵脱保护用于释放荧光素标记的寡核苷酸。尽管6-FAM已普遍用于开发各种qPCR探针，但其荧光受到两个作用限制：1.高度依赖pH值；2.光漂白迅速。目前我们已开发出FAM-xtra 亚磷酰胺解决了这两个局限性，同时完整保留了6-FAM亚磷酰胺的所有优点。 FAM-xtra亚磷酰胺可在相同操作条件下与6-FAM亚磷酰胺完全一样​​使用，而无需进行任何更改。由FAM-xtra亚磷酰胺制成的寡核苷酸具有许多优点：1.FAM-xtra标记的寡核苷酸具有与6-FAM标记的寡核苷酸相同的光谱。 2.FAM-xtra标记的寡核苷酸比相应的FAM-标记的寡核苷酸明亮得多。 3.FAM-xtra标记的寡核苷酸比相应的FAM-标记的寡核苷酸具有更高的光稳定性。4.与相应的FAM标记的寡核苷酸相比，FAM-xtra标记的寡核苷酸的荧光对pH的敏感度低得多。在生理条件下，FAM-xtra标记的寡核苷酸的荧光基本上与pH无关，这使得FAM-xtra标记的寡核苷酸的qPCR探针更加稳定。

参考文献

Development of an Innovative Intradermal siRNA Delivery System Using a Combination of a Functional Stearylated Cytoplasm-Responsive Peptide and a Tight Junction-Opening Peptide.
Authors: Ibaraki, Hisako and Kanazawa, Takanori and Takashima, Yuuki and Okada, Hiroaki and Seta, Yasuo
Journal: Molecules (Basel, Switzerland) (2016)

Non-nucleoside phosphoramidites of xanthene dyes (FAM, JOE, and TAMRA) for oligonucleotide labeling.
Authors: Kvach, Maksim V and Tsybulsky, Dmitry A and Shmanai, Vadim V and Prokhorenko, Igor A and Stepanova, Irina A and Korshun, Vladimir A
Journal: Current protocols in nucleic acid chemistry (2013): Unit 4.55

Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes.
Authors: Kvach, Maksim V and Stepanova, Irina A and Prokhorenko, Igor A and Stupak, Aleksander P and Bolibrukh, Dmitry A and Korshun, Vladimir A and Shmanai, Vadim V
Journal: Bioconjugate chemistry (2009): 1673-82

Synthesis and cellular uptake of fluorescently labeled multivalent hyaluronan disaccharide conjugates of oligonucleotide phosphorothioates.
Authors: Karskela, Marika and Virta, Pasi and Malinen, Melina and Urtti, Arto and Lönnberg, Harri
Journal: Bioconjugate chemistry (2008): 2549-58

Synthesis of HyBeacons and dual-labelled probes containing 2′-fluorescent groups for use in genetic analysis.
Authors: Dobson, Neil and McDowell, David G and French, David J and Brown, Lynda J and Mellor, John M and Brown, Tom
Journal: Chemical communications (Cambridge, England) (2003): 1234-5

Amplified fragment length polymorphism (AFLP) protocol for genotyping the malarial parasite Plasmodium falciparum.
Authors: Rubio, J M and Berzosa, P J and Benito, A
Journal: Parasitology (2001): 331-6

Recovery of an oligonucleotide using silver ions immobilized onto paramagnetic particles.
Authors: Ramírez-Vick, J E and García, A A and Lee, J
Journal: Preparative biochemistry & biotechnology (1998): 243-60

Fluorescent dye phosphoramidite labelling of oligonucleotides.
Authors: Theisen, P and McCollum, C and Andrus, A
Journal: Nucleic acids symposium series (1992): 99-100

ReadiView 绿色/红色 细胞活性染料

	货号	22763	存储条件	在零下15度以下保存, 避免光照
	规格	1000 Tests	价格	6060
	Ex (nm)		Em (nm)
	分子量		溶剂	Water
	产品详细介绍

简要概述

ReadiView 绿色/红色活性染料是一种耐用的细胞染色剂，经过优化，可用于对Countess II FL或Countess 3 FL仪器中的有核细胞进行染色。 它可以方便地与传统的FITC或GFP滤光片组配合使用，例如EVOS GFP荧光灯适用于激发和检测绿色染料，而RFP或Texas Red（TxR）荧光灯适用于细胞红染色。 绿色表示活细胞，而红色表示死细胞。 ReadiView染料可对混合群体中的有核细胞进行特定染色，从而降低了复杂性，并在与适当的EVOS光学立方体匹配时提高了计数与样品间的可重复性。

产品说明书

样本实验方案

将10 µL ReadyCount 活/死活力染色剂与10 µL细胞混合。
在室温下孵育2至5分钟。
将10 µL染色的细胞样品上样至Countess细胞计数仪的玻片中。
在Countess细胞计数仪上读取数据。

图示

图1.用ReadyView 绿色/红色细胞活性染料标记的Jurkat细胞的荧光图像。

参考文献

Drug combinations against Borrelia burgdorferi persisters in vitro: eradication achieved by using daptomycin, cefoperazone and doxycycline.
Authors: Feng, Jie and Auwaerter, Paul G and Zhang, Ying
Journal: PloS one (2015): e0117207

Growth inhibition of cultured marine phytoplankton by toxic algal-derived polyunsaturated aldehydes.
Authors: Ribalet, François and Berges, John A and Ianora, Adrianna and Casotti, Raffaella
Journal: Aquatic toxicology (Amsterdam, Netherlands) (2007): 219-27

An improved method for the selective detection of fungi in hospital waters by solid phase cytometry.
Authors: De Vos, Muriel M and Nelis, Hans J
Journal: Journal of microbiological methods (2006): 557-65

A membrane-immunofluorescent-viability staining technique for the detection of Salmonella spp. from fresh and processed meat samples.
Authors: Duffy, G and Kilbride, B and Sheridan, J J and Blair, I S and McDowell, D A
Journal: Journal of applied microbiology (2000): 587-94

Staining with fluorescein diacetate correlates with hepatocyte function.
Authors: Nyberg, S L and Shatford, R A and Payne, W D and Hu, W S and Cerra, F B
Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission (1993): 56-63

6-ROXtra,酸

简要概述

产品基本信息

货号：386

产品名称：6-ROXtra,酸

规格：25mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：730.77

溶剂：DMSO

激发波长（nm）：578

发射波长（nm）：595

产品介绍

ROX染料具有很强的荧光性，但它们非常不稳定，一些商业样品的保质期只有几个月。 与6-ROX化合物相比，我们的6-ROXtra 染料具有更高的稳定性，同时与生物底物（例如寡核苷酸）缀合时具有与6-ROX基本上相同的光谱特性。 此外，我们的6-ROXtra 染料比基本上不溶于水和水性缓冲液的6-ROX染料水溶性更高。 我们的初步研究表明，在大多数情况下，6-ROXtra衍生的寡核苷酸比相应的6-ROX寡核苷酸更容易纯化。 当与蛋白质缀合时，6-ROX的荧光会被严重淬灭，而6-ROXtra 仍会显示非常强的荧光。

点击查看光谱

参考文献

Aligned Expression of IFI16 and STING Genes in RRMS Patients’ Blood.
Authors: Helbi, Sobhan and Ravanbakhsh, Behnam and Karimi, Mohammad and Kooti, Wesam and Jivad, Nahid
Journal: Endocrine, metabolic & immune disorders drug targets (2020): 878-886

RT-qPCR Detection of Low-Copy HIV RNA with Yin-Yang Probes.
Authors: Kireev, Dmitry E and Farzan, Valentina M and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 27-35

SNPs and transcriptional activity of genes of innate and adaptive immunity at the maternal-fetal interface in woman with preterm labour, associated with preterm premature rupture of membranes.
Authors: Lyubomirskaya, Ekaterina S and Kamyshnyi, Alexandr M and Krut, Yuriy Ya and Smiianov, Vladyslav A and Fedoniuk, Larisa Ya and Romanyuk, Lidiya B and Kravets, Natalya Ya and Mochulska, Oksana M
Journal: Wiadomosci lekarskie (Warsaw, Poland : 1960) (2020): 25-30

Selenoprotein P inhibits cell proliferation and ROX production in HCC cells.
Authors: Wang, Jianxin and Shen, Pei and Liao, Sha and Duan, Lian and Zhu, Dandan and Chen, Jinling and Chen, Liuting and Sun, Xiaolei and Duan, Yinong
Journal: PloS one (2020): e0236491

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64

Novel homo Yin-Yang probes improve sensitivity in RT-qPCR detection of low copy HIV RNA.
Authors: Farzan, Valentina M and Kvach, Maksim V and Aparin, Ilya O and Kireev, Dmitry E and Prikazchikova, Tatiana A and Ustinov, Alexey V and Shmanai, Vadim V and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Talanta (2019): 226-232

Expression of Plasma hsa-miR122 in HBV-Related Hepatocellular Carcinoma (HCC) in Vietnamese Patients.
Authors: Quoc, Nguyen Bao and Phuong, Nguyen Doan Nguyen and Ngan, Tang Kim and Linh, Nguyen Thi Minh and Cuong, Pham Hung and Chau, Nguyen Ngoc Bao
Journal: MicroRNA (Shariqah, United Arab Emirates) (2018): 92-99

Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82

Overexpression of OsERF48 causes regulation of OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance.
Authors: Jung, Harin and Chung, Pil Joong and Park, Su-Hyun and Redillas, Mark Christian Felipe Reveche and Kim, Youn Shic and Suh, Joo-Won and Kim, Ju-Kon
Journal: Plant biotechnology journal (2017): 1295-1308

[Short-term Effect of Roxithromycin on Abundance and Diversity of Ammonia-Oxidizing Microorganisms in Activated Sludge].
Authors: Gao, Jing-Feng and Sun, Li-Xin and Fan, Xiao-Yan and Pan, Kai-Ling and Li, Ding-Chang
Journal: Huan jing ke xue= Huanjing kexue (2017): 2961-2971