ReadiUse 预活化APC-iFluor 750 Tandem 货号2571-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiUse 预活化APC-iFluor 750 Tandem

ReadiUse 预活化APC-iFluor 750 Tandem

ReadiUse 预活化APC-iFluor 750 Tandem    货号2571 货号 2571 存储条件 Multiple
规格 1 mg 价格 4584
Ex (nm) 754 Em (nm) 793
分子量 ~105000 溶剂
产品详细介绍

简要概述

产品基本信息

货号:2571

产品名称:ReadiUse 预活化APC-iFluor 750 Tandem

规格:1mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):651

发射波长(nm):779

 

产品介绍

预活化APC-iFluor 750 Tandem是美国AAT Bioquest生产的蛋白标记染料,别藻蓝蛋白(APC)是从螺旋藻(一种蓝绿藻)中分离的藻胆蛋白。 与其他藻胆蛋白一样,APC是荧光的,具有极高的吸收率和高量子效率。 我们的APC-iFluor 750 Tandem由于其相似的光谱特性,是APC-Cy7®和APC-AlexaFluor®750Tandem的绝佳替代品。 我们的APC-iFluor 750 Tandem比APC-Cy7®Tandem更具光稳定性。 ReadiUse 预活化APC-iFluor 750 Tandem是一种活化的APC蛋白,可以很容易地与抗体结合,比传统的APC具有更高的结合产率。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的ReadiUse 预活化APC-iFluor 750 Tandem。 

点击查看光谱

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

 

相关产品

产品名称 货号
ReadiUse 预活化APC-iFluor 700 Tandem Cat#2570
ReadiUse 预活化RPE-iFluor 750 Tandem Cat#2578

说明书
ReadiUse 预活化APC-iFluor 750 Tandem.pdf

iFluor 790羊抗鼠免疫球蛋白(H+L) 货号16750-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor 790羊抗鼠免疫球蛋白(H+L)

iFluor 790羊抗鼠免疫球蛋白(H+L)

iFluor 790羊抗鼠免疫球蛋白(H+L)    货号16750 货号 16750 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 1944
Ex (nm) 787 Em (nm) 812
分子量 ~150000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:16750

产品名称:iFluor 790羊抗鼠免疫球蛋白(H+L)

规格:1mg

浓度:1mg/ml

储存条件:2-6℃,避光。为了延长该产品的保质期,添加等体积的甘油,使最终浓度为约50%的甘油,并储存在-20℃。

适用:荧光免疫、流式细胞术

保质期:12个月

 

产品物理化学光谱特性

外观:蓝色粉末

溶剂:含水缓冲液

激发波长(nm):782

发射波长(nm):811

 

稀释液推荐

对于免疫荧光,建议的染色浓度为75-750ng / ml。对于流式细胞,建议浓度为1 mL染色缓冲液中500 ng-5μg/百万细胞。为了获得每种应用的最佳性能,需要仔细确定该试剂的最佳浓度。

注意:建议的工作稀释度仅供参考。建议用户使用适当的阳性和阴性对照滴定产品以用于测试。

点击查看光谱

点击查看iFluor系列产品光谱

 

参考文献

Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.
Authors: Jingkun Zhao, Baochi Ou, Hao Feng, Puxiongzhi Wang, Shuai Yin, Congcong Zhu, Shenjie Wang, Chun Chen, Minhua Zheng, Yaping Zong
Journal: Oncotarget (2017)

Cadherin-12 enhances proliferation in colorectal cancer cells and increases progression by promoting EMT
Authors: Junjun Ma, Jingkun Zhao, Jun Lu, Puxiongzhi Wang, Hao Feng, Yaping Zong, Baochi Ou, Minhua Zheng, Aiguo Lu
Journal: Tumor Biology (2016): 1–12

The migration and differentiation of hUC-MSCsCXCR4/GFP encapsulated in BDNF/chitosan scaffolds for brain tissue engineering
Authors: Chuanjun Huang, Longxiang Zhao, Jun Gu, Dekang Nie, Yinan Chen, Hao Zuo, Wei Huan, Jinlong Shi, Jian Chen, Wei Shi
Journal: Biomedical Materials (2016): 035004

Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury
Authors: W Shi, CJ Huang, XD Xu, GH Jin, RQ Huang, JF Huang, YN Chen, SQ Ju, Y Wang, YW Shi
Journal: Acta Biomaterialia (2016): 247–261

 

相关产品

产品名称 货号
iFluor 488羊抗鼠免疫球蛋白(H+L) Cat#16448
iFluor 555羊抗鼠免疫球蛋白(H+L) Cat#16460
iFluor 633羊抗鼠免疫球蛋白(H+L) Cat#16478

说明书
iFluor 790羊抗鼠免疫球蛋白(H+L).pdf

ReadiUse 预活化RPE-iFluor 750 Tandem 货号2578-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiUse 预活化RPE-iFluor 750 Tandem

ReadiUse 预活化RPE-iFluor 750 Tandem

ReadiUse 预活化RPE-iFluor 750 Tandem    货号2578 货号 2578 存储条件 Multiple
规格 1 mg 价格 4584
Ex (nm) 566 Em (nm) 778
分子量 ~240000 溶剂
产品详细介绍

简要概述

产品基本信息

货号:2578

产品名称:ReadiUse 预活化RPE-iFluor 750 Tandem

规格:1mg

储存条件:2-8℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):565

发射波长(nm):779

 

产品介绍

ReadiUse 预活化RPE-iFluor 750 Tandem是美国AAT Bioquest生产的蛋白标记染料,R-藻红蛋白(PE)从红藻中分离。 其主要吸收峰位于565nm,第二峰位于496和545nm。 我们的PE-iFluor 750 Tandem由于其相似的光谱特性,是PE-Cy7®和PE-AlexaFluor®750Tandem的绝佳替代品。 ReadiUse 预活化PE-iFluor 750 Tandem是一种活化的PE蛋白,可以很容易地与抗体结合,比传统的PE具有更高的结合产率。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的ReadiUse 预活化RPE-iFluor 750 Tandem。 

点击查看光谱

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

 

相关产品

产品名称 货号
ReadiUse 预活化RPE-iFluor 647 Tandem Cat#2577
ReadiUse 预活化PE-iFluor 594串联染料 Cat#2584
ReadiUse 预活化APC-iFluor 750 Tandem Cat#2571

说明书
ReadiUse 预活化RPE-iFluor 750 Tandem.pdf

trFluor Eu羊抗鼠免疫球蛋白(H+L) 货号16755-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

trFluor Eu羊抗鼠免疫球蛋白(H+L)

trFluor Eu羊抗鼠免疫球蛋白(H+L)

trFluor Eu羊抗鼠免疫球蛋白(H+L)    货号16755 货号 16755 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 25836
Ex (nm) 346 Em (nm) 617
分子量 ~150000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:16755

产品名称:trFluor Eu羊抗鼠免疫球蛋白(H+L)

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):346

发射波长(nm):617

 

产品介绍

trFluor Eu羊抗鼠免疫球蛋白(H+L)是美国AAT Bioquest生产的荧光标记二抗,许多生物分子自身就具有荧光,它们广泛的存在于生物器官里,如细胞、浆液和其他生物制品中。因此传统的敏感荧光素存在严重的局限性和不足,在进行生物监测分析时,生物分子的自发荧光形成很高的背景干扰。解决的方法是用长寿命的荧光素轭合物它具有高的时间分辨率(延长激发波谱和发射波普的检测)最大程度的减少背景荧光干扰。我们的 trFluor Eu荧光探针具有高灵敏度的能够做到时间分辨检测。在和传统的荧光探针相比(例如Alexa的Fluro和花箐染料), trFluor Eu 荧光探针具有较大的斯托克斯位移和极其长的发射光谱半衰期。同另外的TRF化合物相比我们的 trFluor Eu荧光探针具有较高的稳定性和很高的量子产率,而且可以和生物分子相连接。这一 trFluor Eu  山羊-老鼠 IgG (H+L) 轭合物在连接生物素酰化的第一抗体时,通常用作第二步简接免疫荧光试剂。在以生物素莲亲和素为基础的生物学流式测试或者分析方面他是一个非常有用的工具。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的trFluor Eu羊抗鼠免疫球蛋白(H+L)。 

点击查看光谱

 

参考文献

Development of a time-resolved fluorescence resonance energy transfer assay for cyclin-dependent kinase 4 and identification of its ATP-noncompetitive inhibitors
Authors: Lo MC, Ngo R, Dai K, Li C, Liang L, Lee J, Emkey R, Eksterowicz J, Ventura M, Young SW, Xiao SH.
Journal: Anal Biochem (2012): 368

Time-Resolved Fluorescence Resonance Energy Transfer as a Versatile Tool in the Development of Homogeneous Cellular Kinase Assays
Authors: Saville L, Spais C, Mason JL, Albom MS, Murthy S, Meyer SL, Ator MA, Angeles TS, Husten J.
Journal: Assay Drug Dev Technol. (2012)

A homogeneous single-label time-resolved fluorescence cAMP assay
Authors: Martikkala E, Rozwandowicz-Jansen A, Hanninen P, Petaja-Repo U, Harma H.
Journal: J Biomol Screen (2011): 356

Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1a
Authors: Leyris JP, Roux T, Trinquet E, Verdie P, Fehrentz JA, Oueslati N, Douzon S, Bourrier E, Lamarque L, Gagne D, Galleyrand JC, M’Kadmi C, Martinez J, Mary S, Baneres JL, Marie J.
Journal: Anal Biochem (2011): 253

Oligomerization of the serotonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approach
Authors: Paila YD, Kombrabail M, Krishnamoorthy G, Chattopadhyay A.
Journal: J Phys Chem B (2011): 11439

Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies
Authors: Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A.
Journal: J Biol Chem (2011): 11337

A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization
Authors: Kota S, Scampavia L, Spicer T, Beeler AB, Takahashi V, Snyder JK, Porco JA, Hodder P, Strosberg AD.
Journal: Assay Drug Dev Technol (2010): 96

Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRET
Authors: Alvarez-Curto E, Ward RJ, Pediani JD, Milligan G.
Journal: J Biol Chem (2010): 23318

Steady-state and time-resolved fluorescence quenching with transition metal ions as short-distance probes for protein conformation
Authors: Posokhov YO, Kyrychenko A, Ladokhin AS.
Journal: Anal Biochem (2010): 284

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs
Authors: Visser AJ, Laptenok SP, Visser NV, van Hoek A, Birch DJ, Brochon JC, Borst JW.
Journal: Eur Biophys J (2010): 241

 

相关产品

产品名称 货号
trFluor Tb羊抗鼠免疫球蛋白(H+L) Cat#16519
trFluor Eu羊抗兔免疫球蛋白(H+L) Cat#16668
FITC羊抗鼠免疫球蛋白(H+L) Cat#16852

说明书
trFluor Eu羊抗鼠免疫球蛋白(H+L).pdf

ReadiUse 预活化PE-Cy5 Tandem 货号2580-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiUse 预活化PE-Cy5 Tandem

ReadiUse 预活化PE-Cy5 Tandem

ReadiUse 预活化PE-Cy5 Tandem    货号2580 货号 2580 存储条件 Multiple
规格 1 mg 价格 4584
Ex (nm) 565 Em (nm) 666
分子量 ~240000 溶剂
产品详细介绍

简要概述

产品基本信息

货号:2580

产品名称:ReadiUse 预活化PE-Cy5 Tandem

规格:1mg

储存条件:2-8℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):565

发射波长(nm):670

 

产品介绍

ReadiUse 预活化PE-Cy5 Tandem是美国AAT Bioquest生产的蛋白标记染料,PE-Cy5是流式细胞术中常用的颜色。 其主要吸收峰位于565nm,发射峰位于666nm。 对于这种串联颜色,建议使用682/33 nm和695/40 nm的滤光片组。 AAT Bioquest提供这种预活化的PE-Cy5,以促进PE-Cy5串联结合抗体和其他蛋白质,如链霉抗生物素蛋白和其他二级试剂。 我们的预活化PE-Cy5串联已准备好结合,产生比常规繁琐的基于SMCC的缀合化学高得多的产率。 此外,我们的预活化PE-Cy5串联通过其蛋白质中丰富的氨基与蛋白质缀合,而SMCC化学靶向必须通过抗体还原而再生的硫醇基团。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的ReadiUse 预活化PE-Cy5 Tandem。 

点击查看光谱

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

 

相关产品

产品名称 货号
ReadiUse 预活化PE-Cy7 Tandem Cat#2582

说明书
ReadiUse 预活化PE-Cy5 Tandem.pdf

trFluor Tb羊抗鼠免疫球蛋白(H+L) 货号16756-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

trFluor Tb羊抗鼠免疫球蛋白(H+L)

trFluor Tb羊抗鼠免疫球蛋白(H+L)

trFluor Tb羊抗鼠免疫球蛋白(H+L)    货号16756 货号 16756 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 25836
Ex (nm) 333 Em (nm) 544
分子量 ~150000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:16756

产品名称:trFluor Tb羊抗鼠免疫球蛋白(H+L)

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):330

发射波长(nm):544

 

产品介绍

trFluor Tb羊抗鼠免疫球蛋白(H+L)是美国AAT Bioquest生产的荧光标记二抗,许多生物分子自身就具有荧光,它们广泛的纯在于生物器官里,如细胞、浆液和其他生物制品中。因此传统的敏感荧光素存在严重的局限性和不足,在进行生物监测分析时,生物分子的自发荧光形成很高的背景干扰。解决的方法是用长寿命的荧光素轭合物它具有高的时间分辨率(延长激发波谱和发射波普的检测)最大程度的减少背景荧光干扰。我们的 trFluor Eu荧光探针具有高灵敏度的能够做到时间分辨检测。在和传统的荧光探针相比(例如Alexa的Fluro和花箐染料), trFluor Eu 荧光探针具有较大的斯托克斯位移和极其长的发射光谱半衰期。同另外的TRF化合物相比我们的 trFluor Eu荧光探针具有较高的稳定性和很高的量子产率,而且可以和生物分子相连接。这一 trFluor Eu  山羊-老鼠 IgG (H+L) 轭合物在连接生物素酰化的第一抗体时,通常用作第二步简接免疫荧光试剂。在以生物素莲亲和素为基础的生物学流式测试或者分析方面他是一个非常有用的工具。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的trFluor Tb羊抗鼠免疫球蛋白(H+L)。 

点击查看光谱

 

参考文献

Development of a time-resolved fluorescence resonance energy transfer assay for cyclin-dependent kinase 4 and identification of its ATP-noncompetitive inhibitors
Authors: Lo MC, Ngo R, Dai K, Li C, Liang L, Lee J, Emkey R, Eksterowicz J, Ventura M, Young SW, Xiao SH.
Journal: Anal Biochem (2012): 368

Time-Resolved Fluorescence Resonance Energy Transfer as a Versatile Tool in the Development of Homogeneous Cellular Kinase Assays
Authors: Saville L, Spais C, Mason JL, Albom MS, Murthy S, Meyer SL, Ator MA, Angeles TS, Husten J.
Journal: Assay Drug Dev Technol. (2012)

A homogeneous single-label time-resolved fluorescence cAMP assay
Authors: Martikkala E, Rozwandowicz-Jansen A, Hanninen P, Petaja-Repo U, Harma H.
Journal: J Biomol Screen (2011): 356

Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1a
Authors: Leyris JP, Roux T, Trinquet E, Verdie P, Fehrentz JA, Oueslati N, Douzon S, Bourrier E, Lamarque L, Gagne D, Galleyrand JC, M’Kadmi C, Martinez J, Mary S, Baneres JL, Marie J.
Journal: Anal Biochem (2011): 253

Oligomerization of the serotonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approach
Authors: Paila YD, Kombrabail M, Krishnamoorthy G, Chattopadhyay A.
Journal: J Phys Chem B (2011): 11439

Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies
Authors: Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A.
Journal: J Biol Chem (2011): 11337

A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization
Authors: Kota S, Scampavia L, Spicer T, Beeler AB, Takahashi V, Snyder JK, Porco JA, Hodder P, Strosberg AD.
Journal: Assay Drug Dev Technol (2010): 96

Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRET
Authors: Alvarez-Curto E, Ward RJ, Pediani JD, Milligan G.
Journal: J Biol Chem (2010): 23318

Steady-state and time-resolved fluorescence quenching with transition metal ions as short-distance probes for protein conformation
Authors: Posokhov YO, Kyrychenko A, Ladokhin AS.
Journal: Anal Biochem (2010): 284

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs
Authors: Visser AJ, Laptenok SP, Visser NV, van Hoek A, Birch DJ, Brochon JC, Borst JW.
Journal: Eur Biophys J (2010): 241

 

相关产品

产品名称 货号
trFluor Eu羊抗鼠免疫球蛋白(H+L) Cat#16518
trFluor Tb羊抗兔免疫球蛋白(H+L) Cat#16669
FITC羊抗鼠免疫球蛋白(H+L) Cat#16852

说明书
trFluor Tb羊抗鼠免疫球蛋白(H+L).pdf

ReadiUse 预活化PE-Cy5.5 Tandem 货号2581-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiUse 预活化PE-Cy5.5 Tandem

ReadiUse 预活化PE-Cy5.5 Tandem

ReadiUse 预活化PE-Cy5.5 Tandem    货号2581 货号 2581 存储条件 Multiple
规格 1 mg 价格 4584
Ex (nm) 566 Em (nm) 671
分子量 ~240000 溶剂
产品详细介绍

简要概述

产品基本信息

货号:2581

产品名称:ReadiUse 预活化PE-Cy5.5 Tandem

规格:1mg

储存条件:2-8℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):565

发射波长(nm):700

 

产品介绍

ReadiUse 预活化PE-Cy5.5 Tandem是美国AAT Bioquest生产的蛋白标记染料,PE-Cy5.5是流式细胞术中常用的颜色。 其主要吸收峰位于565nm,发射峰位于~700nm。 对于这种串联颜色,建议使用682/33 nm和695/40 nm的滤光片组。 AAT Bioquest提供这种预活化的PE-Cy5.5,以促进PE-Cy5.5与抗体和其他蛋白质如链霉抗生物素蛋白和其他二级试剂的串联结合。 我们的预活化PE-Cy5.5串联已准备好结合,产生比常规繁琐的基于SMCC的缀合化学高得多的产率。 此外,我们的预活化PE-Cy5.5串联通过其蛋白质丰富的氨基与蛋白质缀合,而SMCC化学靶向必须通过抗体还原再生的硫醇基团。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的ReadiUse 预活化PE-Cy5.5 Tandem。 

点击查看光谱

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

 

相关产品

产品名称 货号
预活化的APC-Cy5.5 Tandem ReadiUse Cat#2586
串联染料PE-Cy5.5 Cat#2613
串联染料APC-Cy5.5 Cat#2622

说明书
ReadiUse 预活化PE-Cy5.5 Tandem.pdf

iFluor 555羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰* 货号16776-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor 555羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*

iFluor 555羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*

iFluor 555羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*    货号16776 货号 16776 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 2604
Ex (nm) 557 Em (nm) 570
分子量 ~150000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:16776

产品名称:iFluor 555羊抗鼠免疫球蛋白(H+L)

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):552

发射波长(nm):567

 

产品介绍

iFluor 555羊抗鼠免疫球蛋白(H+L)是美国AAT Bioquest生产的荧光标记二抗,AAT 生物科技公司的 iFluor 染料在标记蛋白尤其是标记抗体是最佳的。这些染料是非常明亮的并且耐光性好,在标记蛋白质时具有最小的荧光淬灭。他们可以很好的被现有的主要的激光荧光激发仪器(如350、405、488、555和633nm)激发。  iFluor 555山羊抗-老鼠免疫球蛋白( (H+L)轭合物 的荧光激发波长和发射波长分别是 559nm 和 ~569 nm。这些卓越的性能使这一类最新探针家族成为 Alexa Fluor® 555山羊抗-老鼠免疫球蛋白( (H+L)轭合物(Alexa Fluor®是Invitrogee的产品)一个非常优秀的标记探针替代品。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的iFluor 555羊抗鼠免疫球蛋白(H+L)。 

点击查看光谱

点击查看iFluor系列产品光谱

 

参考文献

Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.
Authors: Jingkun Zhao, Baochi Ou, Hao Feng, Puxiongzhi Wang, Shuai Yin, Congcong Zhu, Shenjie Wang, Chun Chen, Minhua Zheng, Yaping Zong
Journal: Oncotarget (2017)

Cadherin-12 enhances proliferation in colorectal cancer cells and increases progression by promoting EMT
Authors: Junjun Ma, Jingkun Zhao, Jun Lu, Puxiongzhi Wang, Hao Feng, Yaping Zong, Baochi Ou, Minhua Zheng, Aiguo Lu
Journal: Tumor Biology (2016): 1–12

The migration and differentiation of hUC-MSCsCXCR4/GFP encapsulated in BDNF/chitosan scaffolds for brain tissue engineering
Authors: Chuanjun Huang, Longxiang Zhao, Jun Gu, Dekang Nie, Yinan Chen, Hao Zuo, Wei Huan, Jinlong Shi, Jian Chen, Wei Shi
Journal: Biomedical Materials (2016): 035004

Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury
Authors: W Shi, CJ Huang, XD Xu, GH Jin, RQ Huang, JF Huang, YN Chen, SQ Ju, Y Wang, YW Shi
Journal: Acta Biomaterialia (2016): 247–261

 

相关产品

产品名称 货号
iFluor 555羊抗兔免疫球蛋白(H+L) Cat#16690
iFluor 647羊抗鼠免疫球蛋白(H+L) Cat#16562
iFluor 488羊抗鼠免疫球蛋白(H+L) Cat#16528

说明书
iFluor 555羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*.pdf

ReadiUse 预活化PE-Cy7 Tandem 货号2582-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiUse 预活化PE-Cy7 Tandem

ReadiUse 预活化PE-Cy7 Tandem

ReadiUse 预活化PE-Cy7 Tandem    货号2582 货号 2582 存储条件 Multiple
规格 1 mg 价格 4584
Ex (nm) 566 Em (nm) 778
分子量 ~240000 溶剂
产品详细介绍

简要概述

产品基本信息

货号:2582

产品名称:ReadiUse 预活化PE-Cy7 Tandem

规格:1mg

储存条件:2-8℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):565

发射波长(nm):780

 

产品介绍

ReadiUse 预活化PE-Cy7 Tandem是美国AAT Bioquest生产的蛋白标记染料,PE-Cy7是流式细胞术中常用的颜色。其主要吸收峰位于565nm,发射峰位于780nm。AAT Bioquest提供这种预活化的PE-Cy7,以促进PE-Cy7串联结合抗体和其他蛋白质,如链霉抗生物素蛋白和其他二级试剂。我们的预活化PE-Cy7串联已准备好结合,产生比常规繁琐的基于SMCC的缀合化学高得多的产率。此外,我们的预活化PE-Cy7串联通过其蛋白质丰富的氨基与蛋白质缀合,而SMCC化学靶向必须通过抗体还原再生的硫醇基团。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的ReadiUse 预活化PE-Cy7 Tandem。 

点击查看光谱

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

 

相关产品

产品名称 货号
ReadiUse 预活化APC-Cy7串联染料 Cat#2587
ReadiUse 预活化PE-Cy5 Tandem Cat#2580

说明书
ReadiUse 预活化PE-Cy7 Tandem.pdf

iFluor™700羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰* 货号16785-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor™700羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*

iFluor™700羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*

iFluor™700羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*    货号16785 货号 16785 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 2604
Ex (nm) 690 Em (nm) 713
分子量 ~150000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:16785

产品名称:iFluor 700羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):693

发射波长(nm):713

 

产品介绍

iFluor 700羊抗鼠免疫球蛋白(H+L)是美国AAT Bioquest生产的荧光标记二抗,AAT 生物科技公司的 iFluor 染料在标记蛋白尤其是标记抗体是最佳的。这些染料是非常明亮的并且耐光性好,在标记蛋白质时具有最小的荧光淬灭。他们可以很好的被现有的主要的激光荧光激发仪器(如350、405、488、555和633nm)激发。  iFluor 700山羊抗-老鼠免疫球蛋白( (H+L)轭合物 的荧光激发波长和发射波长分别是 693nm 和 ~713 nm。这些卓越的性能使这一类最新探针家族成为 Alexa Fluor® 700山羊抗-老鼠免疫球蛋白( (H+L)轭合物(Alexa Fluor®是Invitrogee的产品)一个非常优秀的标记探针替代品。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的iFluor 700羊抗鼠免疫球蛋白(H+L)。 

点击查看光谱

点击查看iFluor系列产品光谱

 

参考文献

Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.
Authors: Jingkun Zhao, Baochi Ou, Hao Feng, Puxiongzhi Wang, Shuai Yin, Congcong Zhu, Shenjie Wang, Chun Chen, Minhua Zheng, Yaping Zong
Journal: Oncotarget (2017)

Cadherin-12 enhances proliferation in colorectal cancer cells and increases progression by promoting EMT
Authors: Junjun Ma, Jingkun Zhao, Jun Lu, Puxiongzhi Wang, Hao Feng, Yaping Zong, Baochi Ou, Minhua Zheng, Aiguo Lu
Journal: Tumor Biology (2016): 1–12

The migration and differentiation of hUC-MSCsCXCR4/GFP encapsulated in BDNF/chitosan scaffolds for brain tissue engineering
Authors: Chuanjun Huang, Longxiang Zhao, Jun Gu, Dekang Nie, Yinan Chen, Hao Zuo, Wei Huan, Jinlong Shi, Jian Chen, Wei Shi
Journal: Biomedical Materials (2016): 035004

Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury
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