荧光素醛[5-FAM醛]
![荧光素醛[5-FAM醛] 货号9000](http://www.jinpanbio.com.cn/wp-content/uploads/2022/01/20220116_61e3dce70e8cb.svg) |
货号 |
9000 |
存储条件 |
在零下15度以下保存, 避免光照 |
| 规格 |
5 mg |
价格 |
3732 |
| Ex (nm) |
493 |
Em (nm) |
517 |
| 分子量 |
550.52 |
溶剂 |
DMSO |
| 产品详细介绍 |
简要概述
产品基本信息
货号：9000
产品名称：荧光素醛[5-FAM醛]
规格：5 mg
储存条件：-15℃避光防潮
保质期：24个月
产品物理化学光谱特性
分子量：445.43
溶剂：DMSO
激发波长(nm)：493
发射波长(nm)：517
消光系数（cm-1 M-1）:83000
校正因子（260nm）:0.32
校正因子 (280 nm)：0.178
产品介绍
荧光素醛是可以与胺，肼或羟胺反应的反应性荧光染料。荧光素是使用最广泛的绿色荧光染料。醛基可与pH 5-9的胺，酰肼或羟胺基反应。与胺反应性琥珀酰亚胺基酯基（NHS）不同，醛可以在酸性pH下与N端胺基反应，这是某些生物缀合反应有时需要的条件。醛与胺基反应形成席夫碱（Schiff base）。用氢化物进一步还原将形成稳定的C-N键。醛与其他基团之间的反应可实现荧光素染料的位点特异性缀合和标记，使其靶向分子上的所需位置。共轭荧光素染料可以很容易地通过普通的荧光仪器在FITC通道下检测到。
点击查看光谱
参考文献
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Journal: Journal of lipid research (2020)
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Journal: Bioconjugate chemistry (2020): 1295-1300
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Preparation and optical parameter characterization of two aldehyde derivative thin films for photonic applications by drop casting method.
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Journal: Luminescence : the journal of biological and chemical luminescence (2020): 903-912
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