CytoTell 活细胞染色红色 590

	货号	22262	存储条件	在零下15度以下保存, 避免光照
	规格	2×500 Tests	价格	3264
	Ex (nm)	562	Em (nm)	587
	分子量	619.53	溶剂	DMSO
	产品详细介绍

简要概述

CytoTell 活细胞染色红色 590是美国AAT Bioquest生产的用于活细胞染色的探针，流式细胞仪结合荧光染色是一个强大的分析异质细胞群工具。在所有现有的荧光染料CFSE是一种广泛用于活细胞分析的优选细胞增殖的指标。然而，这是不可能用CFSE及其荧光素类似物的GFP转染的细胞，或为其中一个FITC标记的抗体用于自CFSE及其荧光素类似物，具有的激发和发射光谱几乎相同。 CytoTell 染料是主要的激光线，如405纳米， 488纳米或633纳米多色排放。 CytoTell 染料具有最小的细胞毒性，并且是用于多色应用与任何GFP的细胞系或FITC-标记的抗体，它们激发或发射光谱的荧光明显不同。 CytoTell 红色是红色荧光染料染色的细胞均匀。 CytoTell 红也可用于标记细胞的长期跟踪。分析使用两个参数的曲线可以更好的提供分辨率。细胞标记的细胞CytoTell 红可以是固定和透化的，适用于使用标准含甲醛固定剂与基于皂苷的透化缓冲液胞内靶标分析。 CytoTell 红色为630nm的激发峰，并且可以通过红色（ 633纳米）的激光线激发。它具有660nm的峰值发射，可以用一个 660/20通道滤波片组（相当于APC的Alexa Fluor ® 647 ，和Cy5 ® ） ，使其与利用GFP或FITC标记的抗体的多色细胞分析中的应用兼容被检测。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的CytoTell 活细胞染色红色 590。 

产品说明书

操作方法

1.准备500 XDMSO储备溶液

将500μLDMSO加入染料粉末小瓶中，通过涡旋混合均匀，得到500X DMSO储备溶液。

注意：应及时使用原液; 任何剩余的溶液应等分并在< -20 ^o C冷冻。避免反复冻融循环，避免光照。

2.准备1X染料工作溶液

制备1X 染料工作由d溶液iluting的500X DMSO储备溶液在1至500   在Hanks（HHBS）和20mM Hepes缓冲液或者您选择的缓冲液，pH 7 （如1种500X的μL DMSO储备溶液至500 μL缓冲区）使用前。通过涡旋将它们充分混合。

注意：染料工作溶液的最终浓度应根据经验确定不同的细胞类型和/或实验条件。建议在至少在折叠范围内的浓度下进行测试。例如CytoTell™Red在某些细胞类型中使用的量可能比推荐浓度少得多。

3.用流式细胞仪或荧光显微镜分析细胞：

3.1用测试化合物处理细胞一段所需的时间。

3.2离心细胞，每管取1-5×10 ^5个细胞。

3.3将细胞重悬于500μL 染料工作溶液中（来自步骤2）。

可选：一个可以添加500X DMSO储备溶液到细胞中的情况下直接介质除去（如，加入1种微升500X DMSO储备溶液加到500个微升细胞）

3.4在室温或37 ° C 下用染料溶液孵育细胞10到30分钟，避光。

3.5从细胞中取出染料工作溶液，用HHBS或您选择的缓冲液清洗细胞。将细胞重悬于500μL预热的HHBS或培养基中，每管获得1-5×10 ^5个细胞。

3.6使用流式细胞仪或荧光显微镜监测推荐的Ex / Em（见表1）的荧光变化。

参考文献

Cooperation of innate immune cells during Hepatitis C virus infection
Authors: Volker Klöss
Journal: (2017)

CXCL12–CXCR4 Axis Is Required for Contact-Mediated Human B Lymphoid and Plasmacytoid Dendritic Cell Differentiation but Not T Lymphoid Generation
Authors: Hirohito Minami, Keiki Nagaharu, Yoshiki Nakamori, Kohshi Ohishi, Naoshi Shimojo, Yuki Kageyama, Takeshi Matsumoto, Yuka Sugimoto, Isao Tawara, Masahiro Masuya
Journal: The Journal of Immunology (2017): ji1700054

Interaction and Mutual Activation of Different Innate Immune Cells Is Necessary to Kill and Clear Hepatitis C Virus-Infected Cells
Authors: Volker Klöss, Oliver Grünvogel, Guido Wabnitz, Tatjana Eigenbrod, Stefanie Ehrhardt, Felix Lasitschka, Volker Lohmann, Alexander H Dalpke
Journal: Frontiers in Immunology (2017): 1238

Onionin A inhibits ovarian cancer progression by suppressing cancer cell proliferation and the protumour function of macrophages
Authors: Junko Tsuboki, Yukio Fujiwara, Hasita Horlad, Daisuke Shiraishi, Toshihiro Nohara, Shingo Tayama, Takeshi Motohara, Yoichi Saito, Tsuyoshi Ikeda, Kiyomi Takaishi
Journal: Scientific Reports (2016)

Multiplexing analysis of cell proliferation and cellular functions using a new multicolor panel of fluorescent cell proliferation dyes (P1290)
Authors: Jinfang Liao, Qin Zhao, Yibo Wu, Zhenjun Diwu
Journal: The Journal of Immunology (2013): 119–4

相关产品

Cell Meter BX520固定化细胞活性染料

	货号	22510	存储条件	在零下15度以下保存, 避免光照
	规格	200 Tests	价格	2472
	Ex (nm)	491	Em (nm)	516
	分子量		溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：22510

产品名称：Cell Meter BX520固色活性染料

规格：200 Tests

储存条件：保存在冰箱-15℃干燥

保质期：12个月

产品物理化学光谱特性

分子量：N/A

激发波长(nm)：491

发射波长(nm)：510

产品介绍 

从活细胞中区分和排除死细胞可以更清晰地分离和鉴定细胞群,Cell Meter BX520固定化细胞活性染料是细胞不可渗透的一类荧光活性染料，它们通过优化以匹配常见流式细胞仪的主要激发激光，例如350、405、488、633和647 nm。这些染料不渗入活细胞，但渗入膜受损的细胞。它们与含胺和硫醇的蛋白质以及其他细胞成分发生不可逆的反应。由于具有受损膜的死细胞或固定细胞更容易与Cell Meter BX520固定化细胞活性染料发生反应，因此比具有完整膜的活细胞的染色更亮，这些染料可用于评估哺乳动物细胞的活与死状态。使用这些染料时，需要考虑一些因素，例如，每种染料的滴定，以确保活细胞几乎没有染色。Cell Meter BX520固定化细胞活性染料通过优化，可以在488 nm的蓝色激发光中激发，并在520 nm（FITC通道）发射。与其他商业上类似的活性染料相比，这种可固定的活性染料更加耐用、稳定。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cell Meter BX520固定化细胞活性染料。 

点击查看光谱

图示

图1.通过Cell Meter 固定化细胞活性染料检测Jurkat细胞活性。处理Jurkat细胞并用Cell Meter BX520（Cat＃22510）染色，然后固定在3.7％甲醛中并通过流式细胞仪进行分析。带有FITC通道的死细胞群体（绿色）很容易与活细胞群体（红色）区分开，在固定之前和之后都获得了几乎相同的结果。

参考文献

CFSE dilution to study human T and NK cell proliferation in vitro.
Authors: Terrén, Iñigo and Orrantia, Ane and Vitallé, Joana and Zenarruzabeitia, Olatz and Borrego, Francisco
Journal: Methods in enzymology (2020): 239-255

Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Cells.
Authors: Azari, Hassan and Deleyrolle, Loic P and Reynolds, Brent A
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 59-67

Estimates and impact of lymphocyte division parameters from CFSE data using mathematical modelling.
Authors: Mazzocco, Pauline and Bernard, Samuel and Pujo-Menjouet, Laurent
Journal: PloS one (2017): e0179768

Analysis of CFSE time-series data using division-, age- and label-structured population models.
Authors: Hross, Sabrina and Hasenauer, Jan
Journal: Bioinformatics (Oxford, England) (2016): 2321-9

Assessment of lymphocyte proliferation for diagnostic purpose: Comparison of CFSE staining, Ki-67 expression and 3H-thymidine incorporation.
Authors: Lašťovička, Jan and Rataj, Michal and Bartůňková, Jiřina
Journal: Human immunology (2016): 1215-1222

Poor association of allergen-specific antibody, T- and B-cell responses revealed with recombinant allergens and a CFSE dilution-based assay.
Authors: Eckl-Dorna, J and Campana, R and Valenta, R and Niederberger, V
Journal: Allergy (2015): 1222-9

Quality control of extracorporeal photochemotherapy: Proliferation assay using CFSE validated according to ISO 15189:2007 standards.
Authors: Faivre, Lionel and Lecouflet, Lucie and Liu, Wang-Qing and Khadher, Isabelle and Lahaie, Camille and Vidal, Michel and Legouvello, Sabine and Beaumont, Jean-Louis and Bierling, Philippe and Rouard, Hélène and Birebent, Brigitte
Journal: Cytometry. Part B, Clinical cytometry (2015): 30-9

Mathematical models for CFSE labelled lymphocyte dynamics: asymmetry and time-lag in division.
Authors: Luzyanina, Tatyana and Cupovic, Jovana and Ludewig, Burkhard and Bocharov, Gennady
Journal: Journal of mathematical biology (2014): 1547-83

Quality control of extracorporeal photochemotherapy: Proliferation assay using CFSE validated according to ISO 15189:2007 standards.
Authors: Lionel, Faivre and Lucie, Lecouflet and Wang-Qing, Liu and Isabelle, Khadher and Camille, Lahaie and Michel, Vidal and Sabine, Legouvello and Jean-Louis, Beaumont and Philippe, Bierling and Hélène, Rouard and Brigitte, Birebent
Journal: Cytometry. Part B, Clinical cytometry (2014)

Asymmetry of Cell Division in CFSE-Based Lymphocyte Proliferation Analysis.
Authors: Bocharov, Gennady and Luzyanina, Tatyana and Cupovic, Jovana and Ludewig, Burkhard
Journal: Frontiers in immunology (2013): 264

Cell Explorer 固定化细胞示踪试剂盒 红色荧光

	货号	22625	存储条件	在零下15度以下保存, 避免光照
	规格	200 Tests	价格	2604
	Ex (nm)	575	Em (nm)	600
	分子量		溶剂
	产品详细介绍

简要概述

Cell Explorer 固定化细胞示踪试剂盒是美国AAT Bioquest生产的用于标记活细胞的试剂盒，我们的Cell Explorer 荧光成像试剂盒是一套用于标记细胞的工具，用于细胞功能的荧光显微镜研究。细胞的有效标记为研究时空背景下的细胞事件提供了一种有力的方法。该特定试剂盒设计用于以红色荧光均匀标记活细胞，用于需要将荧光标签分子保留在细胞中相对较长时间的研究。单元可以固定以保留成像图案。该试剂盒使用带有细胞保留部分的弱荧光染料。染料进入活细胞时会发出强烈的荧光，并被捕获在活细胞内部以在相对较长的时间内提供稳定的荧光信号。该染料是疏水性化合物，易于渗透完整的活细胞。需要最少的动手时间。它可以很容易地适应各种荧光平台，例如微孔板检测，免疫细胞化学和流式细胞仪。它可用于多种研究，包括细胞粘附，趋化性，多药耐药性，细胞生存力，细胞凋亡和细胞毒性。该套件为所有基本组件提供了优化的细胞标记方案。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cell Explorer 固定化细胞示踪试剂盒。

产品说明书

样品实验方案

简要概述

1.准备样品
2.从培养箱中取出细胞板
3.添加每孔10 µL的10X Track It Red工作溶液
4.在室温下将细胞染色15分钟至1小时
5.洗涤细胞
6.在显微镜下在Ex / Em = 570/600 nm下检查样品

溶液配制

1.储备溶液配制

所有未使用的储备溶液应分为一次性使用的等分试样，并在制备后储存在-20°C下。 避免重复冻融循环。
1.1Track It Red DMSO储备溶液（500X）：
将50 µL DMSO（组分C）加入组分A的小瓶中。

2.工作溶液配制

将500X Track It Red DMSO储备溶液稀释到测定缓冲液（组分B）中。 例如，要使一个96孔微孔板的TrackIt Red工作溶液最终浓度达到1X，可将20 µL Track It Red DMSO储备溶液稀释到10 mL的测定缓冲液（组分B）中。 注意：对于不同的细胞类型和/或实验条件，应凭经验确定Track It Red工作溶液的最终浓度。 建议以至少十倍以上的浓度进行测试。 注意：应立即准备和使用工作溶液。远离光线。

点击查看细胞制备方案

样品示例及操作

Cell Meter BX650固定化细胞活性染料

	货号	22520	存储条件	在零下15度以下保存, 避免光照
	规格	200 Tests	价格	2472
	Ex (nm)	518	Em (nm)	654
	分子量	844.83	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：22520

产品名称：Cell Meter BX650固定化细胞活性染料

规格：200 Tests

储存条件：保存在冰箱-15℃干燥

保质期：12个月

产品物理化学光谱特性

分子量：N/A

激发波长(nm)：518

发射波长(nm)：654

产品介绍 

从活细胞中区分和排除死细胞可以更清晰地分离和鉴定细胞群。Cell Meter BX650固定化细胞活性染料是一类细胞不可渗透的荧光活性染料，它们经过优化以匹配常见流式细胞仪的主要激发激光，例如350、405、488、633和647 nm。这些染料不渗入活细胞，但渗入膜受损的细胞。它们与含胺和硫醇的蛋白质以及其他细胞成分发生不可逆的反应。由于具有受损膜的死细胞或固定细胞更容易与Cell Meter BX650固定化细胞活性染料发生反应，因此比具有完整膜的活细胞的染色更亮，因此这些染料可用于评估哺乳动物细胞的活与死状态。使用这些染料时，需要考虑一些因素，例如，每种染料的滴定，以确保活细胞几乎没有染色。Cell Meter BX650固定化细胞活性染料经过优化，可以在488 nm的蓝色激光激发下以650 nm的波长发射。与其他商业上类似的活性染料相比，这种固定化细胞活性染料更加耐用、稳定。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cell Meter BX650固定化细胞活性染料。 

点击查看光谱

图示

图1.通过Cell Meter 固定化细胞活性染料检测Jurkat细胞活性。处理Jurkat细胞并用Cell Meter BX650（Cat＃22520）染色，然后固定在3.7％甲醛中，并通过流式细胞仪进行分析。带有PerCP通道的死细胞群体（蓝色峰）很容易与活细胞群体（红色峰）区分开，在固定之前和之后都获得了几乎相同的结果。

参考文献

Fluorescence-based method is more accurate than counting-based methods for plotting growth curves of adherent cells.
Authors: Pereira, Túlio Felipe and Levin, Gabriel and DeOcesano-Pereira, Carlos and Caodaglio, Amanda Schiersner and Fujita, André and Tonso, Aldo and Sogayar, Mari Cleide
Journal: BMC research notes (2020): 57

[Immune function of myeloid-derived suppressor cells and its mechanism in obstructive sleep apnea syndrome].
Authors: Chen, S W and Li, J and Xiang, B and Xu, S J and Wang, L
Journal: Zhonghua yi xue za zhi (2020): 295-300

CFDA-SE Combined with MACSiBeads™ Particles to Evaluate the Inhibitory Effect of Treg Cells in vitro.
Authors: Ren, Qingqi and Jiang, Chunlin and Liu, Jikui
Journal: Annals of clinical and laboratory science (2019): 740-747

Tracking keratinocytes and melanocytes using carboxyfluorescein hydroxysuccinimidyl ester staining.
Authors: Lönnqvist, Susanna and Junker, Johan P E and Sedell, Maria and Nyman, Erika and Kratz, Gunnar
Journal: PloS one (2019): e0221878

[Effects of skin γδ T lymphocytes on wound healing of mice through regulating proliferation and differentiation of mice epidermal cells].
Authors: Zhu, H J and Li, Y S and Wang, Y P and Hu, X H and Zhang, X R and Qiu, L and He, W F and Luo, G X
Journal: Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns (2019): 298-307

Impaired Immunosuppressive Effect of Bronchoalveolar Mesenchymal Stem Cells in Hypersensitivity Pneumonitis: Preliminary Findings.
Authors: Balogh, Enikő and Nagy, Béla and Gyetvai, Ágnes and Bene, Zsolt and Hendrik, Zoltán and Jeney, Viktória and Nagy, Péter and Papp, Ágnes and Balla, József and Balla, György and Kappelmayer, János and Nagy, Béla
Journal: Cytometry. Part B, Clinical cytometry (2018): 363-368

Delineating the distinct role of AKT in mediating cell survival and proliferation induced by CD154 and IL-4/IL-21 in chronic lymphocytic leukemia.
Authors: Chapman, Elinor A and Oates, Melanie and Mohammad, Ishaque S and Davies, Barry R and Stockman, Paul K and Zhuang, Jianguo and Pettitt, Andrew R
Journal: Oncotarget (2017): 102948-102964

Human auricular chondrocytes with high proliferation rate show high production of cartilage matrix.
Authors: Ishibashi, Makiko and Hikita, Atsuhiko and Fujihara, Yuko and Takato, Tsuyoshi and Hoshi, Kazuto
Journal: Regenerative therapy (2017): 21-28

[Tumor derived IgG suppress the proliferation of T cells in cord blood].
Authors: Liu, E Y and Liu, J F and Shao, W W and Xiao, L and Li, G H and Chang, X H and Qiu, X Y
Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (2017): 824-828

A comparative study of colorimetric cell proliferation assays in immune cells.
Authors: Koyanagi, Madoka and Kawakabe, So and Arimura, Yutaka
Journal: Cytotechnology (2016): 1489-98

Cell Navigator 溶酶体标记试剂盒 近红外荧光

	货号	22652	存储条件	在零下15度以下保存, 避免光照
	规格	500 Tests	价格	2604
	Ex (nm)	636	Em (nm)	651
	分子量		溶剂
	产品详细介绍

简要概述

Cell Navigator 溶酶体标记试剂盒 近红外荧光是一套荧光成像工具，用于标记亚细胞细胞器，如膜，溶酶体，线粒体，细胞核等。活细胞区的选择性标记为研究空间细胞事件提供了一种强大的方法和时间背景。

该特定试剂盒设计用于在Ex / Em =630/650nm处以大斯托克斯位移的近红外荧光标记活细胞的溶酶体。该试剂盒使用专有的溶解性染料，可能通过溶酶体pH梯度选择性地积聚在溶酶体中。溶致指示剂，一种疏水性化合物，很容易渗透完整的活细胞，并被困在溶酶体内。进入溶酶体后，其荧光显着增强。这一关键特征显着降低了其染色背景，使其可用于多种研究，包括细胞粘附，趋化性，多药耐药性，细胞活力，细胞凋亡和细胞毒性。该套件提供所有必要组件。 它适用于悬浮细胞和贴壁细胞。Cell Navigator 溶酶体标记试剂盒 近红外荧光 是美国AAT Bioquest研发的产品。

产品说明书

分析方案

概述

准备细胞

添加染料工作溶液

在37°C孵育30分钟至2小时

在荧光显微镜下Ex / Em = 630 / 650nm（Cy5滤光片组）处进行分析

操作方法

1.准备溶酶体染色溶液：

1.1解冻LysoBrite NIR（组分A）至室温。

1.2通过将20μLLysoBrite NIR（组分A）稀释到10mL活细胞染色缓冲液（组分B）中制备染料工作溶液。

注1：对于一个96孔板，20μLLysoBrite NIR（组分A）就足够了。 将未使用的LysoBrite NIR（组分A）等分并储存在<-20℃。 避光，避免反复冻融循环。

注2：荧光溶酶体指示剂的最佳浓度根据具体应用而变化。 可以根据特定细胞类型和细胞或组织对探针的渗透性来修改染色条件。

2.准备和染色细胞：

2.1对于粘附细胞：在96孔黑色壁/透明底板（100μL/孔/ 96孔板）中或在装有适当培养基的培养皿内的盖玻片上培养细胞。当细胞达到所需的汇合时，加入等体积（如100μL/孔/ 96孔板）的染料加工溶液（来自步骤1.2）。将细胞在37℃，5％CO₂培养箱中孵育30分钟至2小时。使用配有Cy5滤光片的荧光显微镜观察细胞。

注意：如果细胞看起来没有充分染色，建议增加标记浓度或孵育时间以使染料积累。

2.2对于悬浮细胞：以1,000rpm离心细胞5分钟以获得细胞沉淀并吸出上清液。在预热的生长培养基中轻轻重悬细胞沉淀，然后加入等体积的染料加工溶液（来自步骤1.2）。将细胞在37℃，5％CO₂培养箱中孵育30分钟至2小时。使用配有Cy5滤光器的荧光显微镜观察细胞。

注1：如果细胞看起来没有充分染色，建议增加标记浓度或培养时间以使染料积累。

注2：悬浮细胞可以附着在用BD Cell-Tak®（BD Biosciences）处理过的盖玻片上，并作为贴壁细胞染色（见步骤2.1）。

图1.使用具有Cy5过滤器组的Olympus荧光显微镜在Costar黑色96孔板中用Cell Navigator TM溶酶体染色试剂盒染色的Hela细胞的图像。

参考文献

A Triple-Fluorophore Labeled Nucleic Acid pH Nanosensor to Investigate Non-Viral Gene Delivery
Authors: David R Wilson, Denis Routkevitch, Yuan Rui, Arman Mosenia, Karl J Wahlin, Alfredo Quinones-Hinojosa, Donald J Zack, Jordan J Green
Journal: Molecular Therapy (2017)

Silica-based nanoparticles as bi-functional and bi-modal imaging contrast agents
Authors: Séverine Lechevallier, Robert Mauricot, Hélène Gros-Dagnac, Sylviane Chevreux, Gilles Lemercier, Erick Phonesouk, Muriel Golzio, Marc Verelst
Journal: ChemPlusChem (2017)

Decidua-derived mesenchymal stem cells as carriers of mesoporous silica nanoparticles. In vitro and in vivo evaluation on mammary tumors
Authors: Juan L Paris, Paz de la Torre, Miguel Manzano, M Victoria Cabanas, Ana I Flores, María Vallet-Regí
Journal: Acta biomaterialia (2016): 275–282

Rhodamine bound maghemite as a long-term dual imaging nanoprobe of adipose tissue-derived mesenchymal stromal cells
Authors: Vratislav Cmiel, Josef Skopalik, Katerina Polakova, Jan Solar, Marketa Havrdova, David Milde, Ivan Justan, {cmiel2016rhodamine Magro
Journal: European Biophysics Journal (2016): 1–12

Endocytosed β2-microglobulin amyloid fibrils induce necrosis and apoptosis of rabbit synovial fibroblasts by disrupting endosomal/lysosomal membranes: a novel mechanism on the cytotoxicity of amyloid fibrils
Authors: Tadakazu Okoshi, Itaru Yamaguchi, Daisaku Ozawa, Kazuhiro Hasegawa, Hironobu Naiki
Journal: PloS one (2015): e0139330

Fluorescence Imaging of siRNA Delivery by Peptide Nucleic Acid-based Probe
Authors: Takaya Sato, Yusuke Sato, Kenta Iwai, Shusuke Kuge, Norio Teramae, Seiichi Nishizawa
Journal: Analytical Sciences (2015): 315–320

The consideration of indolicidin modification to balance its hemocompatibility and delivery efficiency
Authors: Ching-Wei Tsai, Wei-Wen Hu, Chih-I Liu, Ruoh-Chyu Ruaan, Bing-Chang Tsai, Shiow-Lian Catherine Jin, Yung Chang, Wen-Yih Chen
Journal: International journal of pharmaceutics (2015): 498–505

A monitoring method for Atg4 activation in living cells using peptide-conjugated polymeric nanoparticles
Authors: Kyung-mi Choi, Hae Yun Nam, Jin Hee Na, Seong Who Kim, Sang Yoon Kim, Kwangmeyung Kim, Ick Chan Kwon, Hyung Jun Ahn
Journal: Autophagy (2011): 1052–1062

MagaDye 588-ddTTP

	货号	17061	存储条件	在零下15度以下保存, 避免光照
	规格	5 nmoles	价格	11988
	Ex (nm)	498	Em (nm)	588
	分子量	~1900	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17061

产品名称：MagaDye 588-ddTTP

规格：5nmoles

储存条件：保存在冰箱-15℃干燥

保质期：12个月

产品物理化学光谱特性

Ex(nm)：498

Em(nm)：588

吸收(nm):498

  
产品介绍

Sanger测序，也称为链终止法，是一种基于DNA聚合酶选择性掺入链终止双脱氧核苷酸（ddNTPs）的DNA测序技术。虽然新的NGS技术由于其较高的通量能力和较低的每份样品成本而在临床研究实验室中变得很普遍，但Sanger测序仍具有99.99％的准确度。四种不同的荧光ddNTP（标记为BigDye®，BigDye®是ThermoFisher的商标）是执行Sanger测序的关键成分。MagaDye 588-ddTTP等同于BigDye dROX，具有几乎相同的光谱。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的MagaDye 588-ddTTP。 

点击查看光谱

参考文献

A novel gross deletion and breakpoint junction sequence analysis of ATP7B in a Chinese family with Wilson disease using next‑generation sequencing and Sanger sequencing.
Authors: Liu, Wei-Liang and Li, Fang and Liu, Lu and Chen, Wei and He, Zhi-Xu and Gu, Hao and Ai, Rong
Journal: Molecular medicine reports (2020): 517-523

Concurrent Cultivation of Mycobacterium avium and Mycobacterium intracellulare Identified by a Single Sanger Sequencing of the 16S Gene.
Authors: Han, Xiang Y and Golshan, Mohammad A and Bowman, Christopher J
Journal: Journal of clinical microbiology (2020)

Detection of TERT promoter mutation in serum cell-free DNA using wild-type blocking PCR combined with Sanger sequencing in hepatocellular carcinoma.
Authors: Akuta, Norio and Suzuki, Fumitaka and Kobayashi, Mariko and Fujiyama, Shunichiro and Kawamura, Yusuke and Sezaki, Hitomi and Hosaka, Tetsuya and Kobayashi, Masahiro and Saitoh, Satoshi and Arase, Yasuji and Ikeda, Kenji and Suzuki, Yoshiyuki and Kumada, Hiromitsu
Journal: Journal of medical virology (2020)

Guidelines for Sanger sequencing and molecular assay monitoring.
Authors: Crossley, Beate M and Bai, Jianfa and Glaser, Amy and Maes, Roger and Porter, Elizabeth and Killian, Mary Lea and Clement, Travis and Toohey-Kurth, Kathy
Journal: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (2020): 1040638720905833

Rapid, Inexpensive Measurement of Synthetic Bacterial Community Composition by Sanger Sequencing of Amplicon Mixtures.
Authors: Cermak, Nathan and Datta, Manoshi Sen and Conwill, Arolyn
Journal: iScience (2020): 100915

Shall I trust the report? Variable performance of Sanger sequencing revealed by deep sequencing on HIV drug resistance mutation detection.
Authors: Chen, Nan-Yu and Kao, Shu-Wei and Liu, Zhuo-Hao and Wu, Ting-Shu and Tsai, Chia-Lung and Lin, Hsi-Hsun and Wong, Wing-Wai and Chang, Yea-Yuan and Chen, Shu-Sheng and Ku, Stephane Wen-Wei
Journal: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (2020): 182-191

Update on molecular companion diagnostics – a future in personalized medicine beyond Sanger sequencing.
Authors: Campbell, Michelle Renee
Journal: Expert review of molecular diagnostics (2020)

BEAT: A Python Program to Quantify Base Editing from Sanger Sequencing.
Authors: Xu, Li and Liu, Yakun and Han, Renzhi
Journal: The CRISPR journal (2019): 223-229

Characterization and Clinical Significance of Natural Variability in Hepatitis B Virus Reverse Transcriptase in Treatment-Naive Chinese Patients by Sanger Sequencing and Next-Generation Sequencing.
Authors: Fu, Ya and Zeng, Yongbin and Chen, Tianbin and Chen, Huijuan and Lin, Ni and Lin, Jinpiao and Liu, Xiaofeng and Huang, Er and Wu, Songhang and Wu, Shu and Xu, Siyi and Wang, Long and Ou, Qishui
Journal: Journal of clinical microbiology (2019)

Comparison of Sanger sequencing for hepatitis C virus genotyping with a commercial line probe assay in a tertiary hospital.
Authors: Goletti, Sylvie and Zuyten, Siméon and Goeminne, Léonie and Verhofstede, Chris and Rodriguez-Villalobos, Hector and Bodeus, Monique and Stärkel, Peter and Horsmans, Yves and Kabamba-Mukadi, Benoît
Journal: BMC infectious diseases (2019): 738

Cell Navigator 溶酶体标记试剂盒 绿色荧光

	货号	22656	存储条件	在零下15度以下保存, 避免光照
	规格	500 Tests	价格	1944
	Ex (nm)	501	Em (nm)	510
	分子量		溶剂
	产品详细介绍

简要概述

Cell Navigator 溶酶体标记试剂盒 绿色荧光是一套荧光成像工具，用于标记亚细胞细胞器，如膜，溶酶体，线粒体，细胞核等。活细胞区的选择性标记为研究空间细胞事件提供了一种强大的方法和时间背景。

该特定试剂盒设计用于在Ex / Em =445/503nm处以大斯托克斯位移的绿色荧光标记活细胞的溶酶体。该试剂盒使用专有的溶解性染料，可能通过溶酶体pH梯度选择性地积聚在溶酶体中。溶致指示剂，一种疏水性化合物，很容易渗透完整的活细胞，并被困在溶酶体内。进入溶酶体后，其荧光显着增强。这一关键特征显着降低了其染色背景，使其可用于多种研究，包括细胞粘附，趋化性，多药耐药性，细胞活力，细胞凋亡和细胞毒性。该套件提供所有必要组件。 它适用于悬浮细胞和贴壁细胞。Cell Navigator 溶酶体标记试剂盒 绿色荧光 是美国AAT Bioquest研发的产品。

点击查看光谱

产品说明书

分析方案

概述

准备细胞

添加染料工作溶液

在37°C孵育30分钟至2小时

在荧光显微镜下Ex / Em = 445 / 503nm处进行分析

操作方法

1.准备溶酶体染色溶液：

1.1解冻 Lysolite Green（组分A）至室温。

1.2通过将20μLLysolite Green（组分A）稀释到10mL活细胞染色缓冲液（组分B）中制备染料工作溶液。

注1：对于一个96孔板，20μLLysolite Green（组分A）就足够了。 将未使用的Lysolite Green（组分A）等分并储存在<-20℃。 避光，避免反复冻融循环。

注2：荧光溶酶体指示剂的最佳浓度根据具体应用而变化。 可以根据特定细胞类型和细胞或组织对探针的渗透性来修改染色条件。

2.准备和染色细胞：

2.1对于粘附细胞：在96孔黑色壁/透明底板（100μL/孔/ 96孔板）中或在装有适当培养基的培养皿内的盖玻片上培养细胞。当细胞达到所需的汇合时，加入等体积（如100μL/孔/ 96孔板）的染料加工溶液（来自步骤1.2）。将细胞在37℃，5％CO₂培养箱中孵育30分钟至2小时。使用配有FITC过滤器组的荧光显微镜观察细胞。

注意：如果细胞看起来没有充分染色，建议增加标记浓度或孵育时间以使染料积累。

2.2对于悬浮细胞：以1,000rpm离心细胞5分钟以获得细胞沉淀并吸出上清液。在预热的生长培养基中轻轻重悬细胞沉淀，然后加入等体积的染料加工溶液（来自步骤1.2）。将细胞在37℃，5％CO₂培养箱中孵育30分钟至2小时。使用配有FITC过滤器组的荧光显微镜观察细胞。

注1：如果细胞看起来没有充分染色，建议增加标记浓度或培养时间以使染料积累。

注2：悬浮细胞可以附着在用BD Cell-Tak®（BD Biosciences）处理过的盖玻片上，并作为贴壁细胞染色（见步骤2.1）。

图1.使用Cell Navigator 溶酶体染色试剂盒染色的U2OS细胞图像* Costar黑色96孔板中的绿色荧光*

参考文献

A Triple-Fluorophore Labeled Nucleic Acid pH Nanosensor to Investigate Non-Viral Gene Delivery
Authors: David R Wilson, Denis Routkevitch, Yuan Rui, Arman Mosenia, Karl J Wahlin, Alfredo Quinones-Hinojosa, Donald J Zack, Jordan J Green
Journal: Molecular Therapy (2017)

Silica-based nanoparticles as bi-functional and bi-modal imaging contrast agents
Authors: Séverine Lechevallier, Robert Mauricot, Hélène Gros-Dagnac, Sylviane Chevreux, Gilles Lemercier, Erick Phonesouk, Muriel Golzio, Marc Verelst
Journal: ChemPlusChem (2017)

Decidua-derived mesenchymal stem cells as carriers of mesoporous silica nanoparticles. In vitro and in vivo evaluation on mammary tumors
Authors: Juan L Paris, Paz de la Torre, Miguel Manzano, M Victoria Cabanas, Ana I Flores, María Vallet-Regí
Journal: Acta biomaterialia (2016): 275–282

Rhodamine bound maghemite as a long-term dual imaging nanoprobe of adipose tissue-derived mesenchymal stromal cells
Authors: Vratislav Cmiel, Josef Skopalik, Katerina Polakova, Jan Solar, Marketa Havrdova, David Milde, Ivan Justan, {cmiel2016rhodamine Magro
Journal: European Biophysics Journal (2016): 1–12

Endocytosed β2-microglobulin amyloid fibrils induce necrosis and apoptosis of rabbit synovial fibroblasts by disrupting endosomal/lysosomal membranes: a novel mechanism on the cytotoxicity of amyloid fibrils
Authors: Tadakazu Okoshi, Itaru Yamaguchi, Daisaku Ozawa, Kazuhiro Hasegawa, Hironobu Naiki
Journal: PloS one (2015): e0139330

Fluorescence Imaging of siRNA Delivery by Peptide Nucleic Acid-based Probe
Authors: Takaya Sato, Yusuke Sato, Kenta Iwai, Shusuke Kuge, Norio Teramae, Seiichi Nishizawa
Journal: Analytical Sciences (2015): 315–320

The consideration of indolicidin modification to balance its hemocompatibility and delivery efficiency
Authors: Ching-Wei Tsai, Wei-Wen Hu, Chih-I Liu, Ruoh-Chyu Ruaan, Bing-Chang Tsai, Shiow-Lian Catherine Jin, Yung Chang, Wen-Yih Chen
Journal: International journal of pharmaceutics (2015): 498–505

A monitoring method for Atg4 activation in living cells using peptide-conjugated polymeric nanoparticles
Authors: Kyung-mi Choi, Hae Yun Nam, Jin Hee Na, Seong Who Kim, Sang Yoon Kim, Kwangmeyung Kim, Ick Chan Kwon, Hyung Jun Ahn
Journal: Autophagy (2011): 1052–1062

相关产品

Cell Navigator 溶酶体标记试剂盒 红色荧光

	货号	22658	存储条件	在零下15度以下保存, 避免光照
	规格	500 Tests	价格	1944
	Ex (nm)	576	Em (nm)	596
	分子量		溶剂
	产品详细介绍

简要概述

Cell Navigator 溶酶体标记试剂盒 红色荧光 是一套荧光成像工具，用于标记亚细胞细胞器，如膜，溶酶体，线粒体，细胞核等。活细胞区的选择性标记为研究空间细胞事件提供了一种强大的方法和时间背景。

该特定试剂盒设计用于在Ex / Em =575/597nm处以大斯托克斯位移的红色荧光标记活细胞的溶酶体。该试剂盒使用专有的溶解性染料，可能通过溶酶体pH梯度选择性地积聚在溶酶体中。该指示剂中的溶质，是一种疏水性化合物，很容易渗透到完整的活细胞，并被保留在溶酶体内一段时间。进入溶酶体后，其荧光显著增强。这一关键特征显著降低了其染色背景，使其可用于多种研究，包括细胞粘附，趋化性，多药耐药性，细胞活力，细胞凋亡和细胞毒性。该试剂盒提供所有必要组件。 它适用于悬浮细胞和贴壁细胞。Cell Navigator 溶酶体标记试剂盒 红色荧光 是美国AAT Bioquest研发的产品。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cell Navigator 溶酶体标记试剂盒。 

点击查看光谱

适用仪器

产品说明书

试验方案

1. 准备细胞
2. 添加染料工作溶液
3. 在37°C孵育30分钟至2小时
4. 在荧光显微镜下Ex / Em = 575 / 597nm处进行分析

注意：1）实验前，取出转染试剂进行室温冻结。

样品示例及使用方法

1.准备溶酶体染色溶液：

1.1  取出试剂盒中A、B组分，室温避光静置5-10min。

1.2  移取20μLLysoBrite Red（组分A），用10mL活细胞染色缓冲液（组分B）进行稀释，制备染料工作液。

注意：1) 20μL的500XLysoBrite Red （组分A）足够完成一个96孔板，根据要求分装并储存未使用的500XLysoBrite Red（组分A）,避免反复冻融。

        2)荧光溶酶体指示剂的最佳浓度根据实际应用而改变，可以根据探针的渗透性对细胞类型、细胞或组织的染色条件进行调整。

2.细胞染色：

2.1对于贴壁细胞：

在96黑色/透明微孔板（100μL/孔/ 96孔板）或装有适当培养基内的培养皿的盖玻片上培养细胞，当细胞达到所需的汇合时，在96孔板或培养皿内加入等量的LysoBrite Red工作液，在37℃、5％CO₂条件下培养箱中孵育30分钟，用预温(37°C) Hanks和20 mM Hepes缓冲液(HBSS)或自配的缓冲液清洗细胞两次，用HBSS或生长培养基填充细胞孔，再使用配有TRITC过滤器组的荧光显微镜观察细胞，使之带红色荧光(Ex/Em = 575/600 nm)。

注意：1）如果细胞看起来没有充分染色，建议适当增加染料浓度或孵育时间。

2.2对于悬浮细胞：

向细胞中加入等量的LysoBrite Red工作液。在37℃、5％CO₂条件下培养箱中孵育30分钟，用预温(37°C) Hanks和20 mM Hepes缓冲液(HBSS)或自配的缓冲液清洗细胞两次，用HBSS或生长培养基填充细胞孔，再使用配有TRITC过滤器组的荧光显微镜观察细胞，使之带红色荧光(Ex/Em = 575/600 nm)。

注意:  1）如果细胞看起来没有充分染色，建议适当增加染料浓度或孵育时间。

         2）悬浮细胞可以附着在用BD Cell-Tak®（BD Biosciences）处理过的盖玻片上，并作为贴壁细胞染色。

示例数据分析及图示

图一：在Costar黑色透明底96孔板中用Cell Navigator 溶酶体染色试剂盒染色Hela细胞的荧光成像结果。

图二：HeLa细胞分别用A和B，置于Costar黑壁/透明底96孔板中染色后的荧光成像结果，在0秒和120秒的曝光时间下，使用Olympus荧光显微镜对信号进行比较。A: Cell Navigator溶酶体染色试剂盒(Cat# 22658)、B: LysoTracker®Red DND-99(来自Invitrogen公司)。 A: Cell Navigator溶酶体染色试剂盒(Cat# 22659)、B: LysoTracker®Red DND-99(来自Invitrogen公司)。

参考文献 

Visualising the dynamics of live pancreatic microtumours self-organised through cell-in-cell invasion
Authors: Yukiko Miyatake, Kaori Kuribayashi-Shigetomi, Yusuke Ohta, Shunji Ikeshita, Agus Subagyo, Kazuhisa Sueoka, Akira Kakugo, Maho Amano, Toshiyuki Takahashi, Takaharu Okajima
Journal: Scientific reports (2018): 14054

A Triple-Fluorophore Labeled Nucleic Acid pH Nanosensor to Investigate Non-Viral Gene Delivery
Authors: David R Wilson, Denis Routkevitch, Yuan Rui, Arman Mosenia, Karl J Wahlin, Alfredo Quinones-Hinojosa, Donald J Zack, Jordan J Green
Journal: Molecular Therapy (2017)

Silica-based nanoparticles as bi-functional and bi-modal imaging contrast agents
Authors: Séverine Lechevallier, Robert Mauricot, Hélène Gros-Dagnac, Sylviane Chevreux, Gilles Lemercier, Erick Phonesouk, Muriel Golzio, Marc Verelst
Journal: ChemPlusChem (2017)

Decidua-derived mesenchymal stem cells as carriers of mesoporous silica nanoparticles. In vitro and in vivo evaluation on mammary tumors
Authors: Juan L Paris, Paz de la Torre, Miguel Manzano, M Victoria Cabanas, Ana I Flores, María Vallet-Regí
Journal: Acta biomaterialia (2016): 275–282

Rhodamine bound maghemite as a long-term dual imaging nanoprobe of adipose tissue-derived mesenchymal stromal cells
Authors: Vratislav Cmiel, Josef Skopalik, Katerina Polakova, Jan Solar, Marketa Havrdova, David Milde, Ivan Justan, {cmiel2016rhodamine Magro
Journal: European Biophysics Journal (2016): 1–12

Endocytosed β2-microglobulin amyloid fibrils induce necrosis and apoptosis of rabbit synovial fibroblasts by disrupting endosomal/lysosomal membranes: a novel mechanism on the cytotoxicity of amyloid fibrils
Authors: Tadakazu Okoshi, Itaru Yamaguchi, Daisaku Ozawa, Kazuhiro Hasegawa, Hironobu Naiki
Journal: PloS one (2015): e0139330

Fluorescence Imaging of siRNA Delivery by Peptide Nucleic Acid-based Probe
Authors: Takaya Sato, Yusuke Sato, Kenta Iwai, Shusuke Kuge, Norio Teramae, Seiichi Nishizawa
Journal: Analytical Sciences (2015): 315–320

The consideration of indolicidin modification to balance its hemocompatibility and delivery efficiency
Authors: Ching-Wei Tsai, Wei-Wen Hu, Chih-I Liu, Ruoh-Chyu Ruaan, Bing-Chang Tsai, Shiow-Lian Catherine Jin, Yung Chang, Wen-Yih Chen
Journal: International journal of pharmaceutics (2015): 498–505

A monitoring method for Atg4 activation in living cells using peptide-conjugated polymeric nanoparticles
Authors: Kyung-mi Choi, Hae Yun Nam, Jin Hee Na, Seong Who Kim, Sang Yoon Kim, Kwangmeyung Kim, Ick Chan Kwon, Hyung Jun Ahn
Journal: Autophagy (2011): 1052–1062

相关产品

MagaDye 588-ddTTP

	货号	17065	存储条件
	规格	50 nmoles	价格	62412
	Ex (nm)	498	Em (nm)	588
	分子量	~1900	溶剂
	产品详细介绍

简要概述

产品基本信息

货号：17065

产品名称：MagaDye 588-ddTTP

规格：50nmoles

储存条件：保存在冰箱-15℃干燥

保质期：12个月

产品物理化学光谱特性

Ex(nm)：498

Em(nm)：588

吸收(nm):498

  
产品介绍

Sanger测序，也称为链终止法，是一种基于DNA聚合酶选择性掺入链终止双脱氧核苷酸（ddNTPs）的DNA测序技术。虽然新的NGS技术由于其较高的通量能力和较低的每份样品成本而在临床研究实验室中变得很普遍，但Sanger测序仍具有99.99％的准确度。四种不同的荧光ddNTP（标记为BigDye®，BigDye®是ThermoFisher的商标）是执行Sanger测序的关键成分。MagaDye 588-ddTTP等同于BigDye dROX，具有几乎相同的光谱。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的MagaDye 588-ddTTP。 

点击查看光谱

参考文献

A novel gross deletion and breakpoint junction sequence analysis of ATP7B in a Chinese family with Wilson disease using next‑generation sequencing and Sanger sequencing.
Authors: Liu, Wei-Liang and Li, Fang and Liu, Lu and Chen, Wei and He, Zhi-Xu and Gu, Hao and Ai, Rong
Journal: Molecular medicine reports (2020): 517-523

Concurrent Cultivation of Mycobacterium avium and Mycobacterium intracellulare Identified by a Single Sanger Sequencing of the 16S Gene.
Authors: Han, Xiang Y and Golshan, Mohammad A and Bowman, Christopher J
Journal: Journal of clinical microbiology (2020)

Detection of TERT promoter mutation in serum cell-free DNA using wild-type blocking PCR combined with Sanger sequencing in hepatocellular carcinoma.
Authors: Akuta, Norio and Suzuki, Fumitaka and Kobayashi, Mariko and Fujiyama, Shunichiro and Kawamura, Yusuke and Sezaki, Hitomi and Hosaka, Tetsuya and Kobayashi, Masahiro and Saitoh, Satoshi and Arase, Yasuji and Ikeda, Kenji and Suzuki, Yoshiyuki and Kumada, Hiromitsu
Journal: Journal of medical virology (2020)

Guidelines for Sanger sequencing and molecular assay monitoring.
Authors: Crossley, Beate M and Bai, Jianfa and Glaser, Amy and Maes, Roger and Porter, Elizabeth and Killian, Mary Lea and Clement, Travis and Toohey-Kurth, Kathy
Journal: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (2020): 1040638720905833

Rapid, Inexpensive Measurement of Synthetic Bacterial Community Composition by Sanger Sequencing of Amplicon Mixtures.
Authors: Cermak, Nathan and Datta, Manoshi Sen and Conwill, Arolyn
Journal: iScience (2020): 100915

Shall I trust the report? Variable performance of Sanger sequencing revealed by deep sequencing on HIV drug resistance mutation detection.
Authors: Chen, Nan-Yu and Kao, Shu-Wei and Liu, Zhuo-Hao and Wu, Ting-Shu and Tsai, Chia-Lung and Lin, Hsi-Hsun and Wong, Wing-Wai and Chang, Yea-Yuan and Chen, Shu-Sheng and Ku, Stephane Wen-Wei
Journal: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (2020): 182-191

Update on molecular companion diagnostics – a future in personalized medicine beyond Sanger sequencing.
Authors: Campbell, Michelle Renee
Journal: Expert review of molecular diagnostics (2020)

BEAT: A Python Program to Quantify Base Editing from Sanger Sequencing.
Authors: Xu, Li and Liu, Yakun and Han, Renzhi
Journal: The CRISPR journal (2019): 223-229

Characterization and Clinical Significance of Natural Variability in Hepatitis B Virus Reverse Transcriptase in Treatment-Naive Chinese Patients by Sanger Sequencing and Next-Generation Sequencing.
Authors: Fu, Ya and Zeng, Yongbin and Chen, Tianbin and Chen, Huijuan and Lin, Ni and Lin, Jinpiao and Liu, Xiaofeng and Huang, Er and Wu, Songhang and Wu, Shu and Xu, Siyi and Wang, Long and Ou, Qishui
Journal: Journal of clinical microbiology (2019)

Comparison of Sanger sequencing for hepatitis C virus genotyping with a commercial line probe assay in a tertiary hospital.
Authors: Goletti, Sylvie and Zuyten, Siméon and Goeminne, Léonie and Verhofstede, Chris and Rodriguez-Villalobos, Hector and Bodeus, Monique and Stärkel, Peter and Horsmans, Yves and Kabamba-Mukadi, Benoît
Journal: BMC infectious diseases (2019): 738

MagaDye 561-ddATP

	货号	17062	存储条件	在零下15度以下保存, 避免光照
	规格	5 nmoles	价格	11988
	Ex (nm)	498	Em (nm)	561
	分子量	~2000	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17062

产品名称：MagaDye 561-ddATP

规格：5nmoles

储存条件：保存在冰箱-15℃干燥

保质期：12个月

产品物理化学光谱特性

Ex(nm)：498

Em(nm)：561

吸收(nm):498

  
产品介绍

Sanger测序，也称为链终止法，是一种基于DNA聚合酶选择性掺入链终止双脱氧核苷酸（ddNTPs）的DNA测序技术。虽然新的NGS技术由于其较高的通量能力和较低的每份样品成本而在临床研究实验室中变得很普遍，但Sanger测序仍具有99.99％的准确度。四种不同的荧光ddNTP（标记为BigDye®，BigDye®是ThermoFisher的商标）是执行Sanger测序的关键成分。MagaDye 561-ddATP等同于BigDye dROX，具有几乎相同的光谱。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的MagaDye 561-ddATP。 

点击查看光谱

参考文献

A novel gross deletion and breakpoint junction sequence analysis of ATP7B in a Chinese family with Wilson disease using next‑generation sequencing and Sanger sequencing.
Authors: Liu, Wei-Liang and Li, Fang and Liu, Lu and Chen, Wei and He, Zhi-Xu and Gu, Hao and Ai, Rong
Journal: Molecular medicine reports (2020): 517-523

Concurrent Cultivation of Mycobacterium avium and Mycobacterium intracellulare Identified by a Single Sanger Sequencing of the 16S Gene.
Authors: Han, Xiang Y and Golshan, Mohammad A and Bowman, Christopher J
Journal: Journal of clinical microbiology (2020)

Detection of TERT promoter mutation in serum cell-free DNA using wild-type blocking PCR combined with Sanger sequencing in hepatocellular carcinoma.
Authors: Akuta, Norio and Suzuki, Fumitaka and Kobayashi, Mariko and Fujiyama, Shunichiro and Kawamura, Yusuke and Sezaki, Hitomi and Hosaka, Tetsuya and Kobayashi, Masahiro and Saitoh, Satoshi and Arase, Yasuji and Ikeda, Kenji and Suzuki, Yoshiyuki and Kumada, Hiromitsu
Journal: Journal of medical virology (2020)

Guidelines for Sanger sequencing and molecular assay monitoring.
Authors: Crossley, Beate M and Bai, Jianfa and Glaser, Amy and Maes, Roger and Porter, Elizabeth and Killian, Mary Lea and Clement, Travis and Toohey-Kurth, Kathy
Journal: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (2020): 1040638720905833

Rapid, Inexpensive Measurement of Synthetic Bacterial Community Composition by Sanger Sequencing of Amplicon Mixtures.
Authors: Cermak, Nathan and Datta, Manoshi Sen and Conwill, Arolyn
Journal: iScience (2020): 100915

Shall I trust the report? Variable performance of Sanger sequencing revealed by deep sequencing on HIV drug resistance mutation detection.
Authors: Chen, Nan-Yu and Kao, Shu-Wei and Liu, Zhuo-Hao and Wu, Ting-Shu and Tsai, Chia-Lung and Lin, Hsi-Hsun and Wong, Wing-Wai and Chang, Yea-Yuan and Chen, Shu-Sheng and Ku, Stephane Wen-Wei
Journal: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (2020): 182-191

Update on molecular companion diagnostics – a future in personalized medicine beyond Sanger sequencing.
Authors: Campbell, Michelle Renee
Journal: Expert review of molecular diagnostics (2020)

BEAT: A Python Program to Quantify Base Editing from Sanger Sequencing.
Authors: Xu, Li and Liu, Yakun and Han, Renzhi
Journal: The CRISPR journal (2019): 223-229

Characterization and Clinical Significance of Natural Variability in Hepatitis B Virus Reverse Transcriptase in Treatment-Naive Chinese Patients by Sanger Sequencing and Next-Generation Sequencing.
Authors: Fu, Ya and Zeng, Yongbin and Chen, Tianbin and Chen, Huijuan and Lin, Ni and Lin, Jinpiao and Liu, Xiaofeng and Huang, Er and Wu, Songhang and Wu, Shu and Xu, Siyi and Wang, Long and Ou, Qishui
Journal: Journal of clinical microbiology (2019)

Comparison of Sanger sequencing for hepatitis C virus genotyping with a commercial line probe assay in a tertiary hospital.
Authors: Goletti, Sylvie and Zuyten, Siméon and Goeminne, Léonie and Verhofstede, Chris and Rodriguez-Villalobos, Hector and Bodeus, Monique and Stärkel, Peter and Horsmans, Yves and Kabamba-Mukadi, Benoît
Journal: BMC infectious diseases (2019): 738