Harlan TD.95027低钙饲料(0.4%P)
Harlan货号：TD.95027
英文名称：Low Calcium Diet (0.4% P)
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。
Formula                                                                     g/Kg
Casein                                                                    200.0
Cystine                                                                     3.0
Sucrose                                                            342.188
Corn Starch                                                         320.0
Soybean Oil                                                          60.0
Cellulose                                                              40.0
Mineral Mix, Ca-P Deficient (79055)                    13.37
Potassium Phosphate, monobasic                     11.43
Vitamin Mix, Teklad (40060)                                10.0
Ethoxyquin, antioxidant                                       0.012
Key Features
+ Purified Diet
+ Calcium
+ Phosphorus
Key Planning Information
+ Products are made fresh to order
+Store product at 4°C or lower
+Use within 6 months (applicable to most diets)
+Box labeled with product name, manufacturing date, and lot number
+ Replace diet at minimum once per week More frequent replacement may be advised
+ Lead time:
· 2 weeks non-irradiated
· 4 weeks irradiated
上海金畔生物科技有限公司（www.jinpanbio.com）提供生命科学研究领域系列产品，包括生化试剂、诊断试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料；修饰性PEG（Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG）；Sigma、Amresco、TCI、MP bio生化试剂；WAKO日本和光纯药、日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA；Megazyme食品分析检测试剂盒、日本共立理化学；Research diets、Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料；Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基；免疫诊断试剂包括:Bethyl抗体；Biolegend流式抗体、Abcam、CST、Santa Cruz抗体；Roche、TOYOBO、NEB品牌的酶；中检所、TRC、药典USP、EP、Reagecon标准品；耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。
上海金畔生物科技有限公司
服务热线:18301939375   QQ：3258089810    3259632176
Email:  info@jinpanbio.com
官网：http://www.jinpanbio.com

Harlan teklad品牌饲料分类
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。
Teklad diet, bedding and enrichment + Teklad laboratory animal diets + Custom research diets
Custom diets are developed for a specific purpose and benefit from your input and our expertise
Determining the most appropriate diet for your research model can be challenging, but we are here to help. With more than 20,000 unique formulas in our database attained over 40 years, Envigo nutritionists have vast experience to draw upon. To meet the progressive demands of science and to serve you better, we continually add to our database as we acquire new knowledge.
Common uses for custom diets include:
Control Nutrients
Vitamin or mineral adjusted
Protein or amino acid adjusted
Lipid or fatty acid adjusted
Induce Disease
Atherogenic (cholesterol, fat, cholate)
Diet-induced obesity (40-60% kcal from fat)
High carbohydrate (fructose, sucrose)
NaCl adjusted
Cuprizone demyelination
Dose Animals
Control gene expression – doxycycline or tamoxifen containing diets
Addition of customer-supplied ingredients/compounds
You can select from the wide variety of existing diets, or one designed specifically for your purposes. Either way, we encourage you to contact us for complimentary consultation with our nutritionists.
A small number of representative custom research diet formulas are found on our website. If you are unable to find a product code or formula description cited in a publication, or one used previously by your lab or others, contact us.
定制饮食是为了特定的目的而制定的，并得益于您的投入和我们的专业知识。
为你的研究模式确定最合适的饮食是很有挑战性的，但我们是来帮忙的。在我们的数据库中，已有超过20，000种独特的配方，40多年来，Envigo营养学家有着丰富的经验可供借鉴。为了满足科学进步的需求，并更好地为您服务，我们不断地在我们的数据库中添加新的知识。
习惯饮食的常见用途包括：
控制养分
维生素或矿物调整后
蛋白或氨基酸调整后
脂或脂肪酸调整后
诱发病
动脉粥样硬化(胆固醇、脂肪、胆酸盐)
饮食型肥胖(脂肪40%-60千卡)
高碳水化合物(果糖，蔗糖)
NaCl调节
铜带脱髓鞘
剂量动物
控制基因表达-强力霉素或他莫昔芬含粮
加法客户供应的配料/化合物
你可以从各种各样的现有饮食中选择，或者是专门为你的目的而设计的。无论哪种方式，我们都鼓励您与我们的营养学家进行免费咨询。
Harlan teklad品牌饲料分类

Harlan氨基酸调节饲料 Amino acid defined
品牌：Harlan
货号：TD.90262
英文名称：Amino acid defined
Harlan氨基酸调节饲料 Amino acid defined
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。
Amino acid defined | Harlan
A few amino acid defined formulas are shown below. This type of diet is used when a diet needs to be deficient in one or more amino acids, or adjustments need to be made to specific amino acid levels.
Formula examples:
TD.99366      Amino acid diet
TD.90262      Methionine and choline deficient diet
TD.00434      Folic acid deficient diet
TD.99386      No lysine AA diet
Sometimes this type of diet is used to further reduce the background level of certain vitamins that are found in protein sources such as casein or vitamin-free (alcohol extracted) casein. Some folic acid deficient diets utilize this approach.
The amino acid profile for most amino acid defined diets is based on work by Harper and Rogers (J. Nutr. (1965) 87:267-276) and is not representative of the amino acid profile of an intact protein source such as casein. Other amino acid profiles can be specified. When an amino acid(s) is removed or reduced, isonitrogenous adjustment can be made if required.
公式实例:
TD.99366氨基酸饮食
TD.90262蛋氨酸和胆碱缺乏饮食
TD.00434叶酸缺乏饮食
TD.99386不含赖氨酸AA饮食
有时，这种饮食被用来进一步降低某些维生素的背景水平，这些维生素存在于蛋白质来源，如酪蛋白或无维生素的酪蛋白(醇提取的)酪蛋白中。一些叶酸缺乏的饮食使用这种方法。
大多数氨基酸定义的饮食的氨基酸谱是基于哈珀和罗杰斯的工作(J.Nutr)。(1965)87：267-276)，不代表酪蛋白等完整蛋白质来源的氨基酸谱。还可以指定其他氨基酸谱。当氨基酸被去除或减少时，如果需要，可以进行等氮调节。
上海金畔生物科技有限公司（www.jinpanbio.com）提供生命科学研究领域系列产品，包括生化试剂、诊断试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料；修饰性PEG（Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG）；Sigma、Amresco、TCI、MP bio生化试剂；WAKO日本和光纯药、日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA；Megazyme食品分析检测试剂盒、日本共立理化学；Research diets、Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料；Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基；免疫诊断试剂包括:Bethyl抗体；Biolegend流式抗体、Abcam、CST、Santa Cruz抗体；Roche、TOYOBO、NEB品牌的酶；中检所、TRC、药典USP、EP、Reagecon标准品；耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。
上海金畔生物科技有限公司
服务热线:18301939375   QQ：3258089810    3259632176
Email:  info@jinpanbio.com
官网：http://www.jinpanbio.com

Harlan矿物质调节饲料Mineral adjusted diets
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。
Mineral adjusted diets
A few mineral adjusted formulas are shown below. Please contact us for additional formulas of this nature or for more information about altering the mineral profile of a diet. Read below for more information.
Formula examples:
TD.80396      Iron deficient diet
TD.85419      Zinc deficient diet
TD.88239      Potassium deficient diet
TD.95027      Low calcium diet (0.4% P)
TD.96348      Diet (20% lactose, 2% Ca, 1.25% P)
TD.92163      Selenium deficient diet
TD.95125      Iodine deficient (0.15% PTU)
Unique protein ingredients may be necessary to minimize background levels of certain minerals. Other steps taken to minimize background mineral contamination include the use of reagent grade minerals when necessary or omission of certain ingredients. Specialized mineral premixes may be used in these diets, or minerals are added individually to the diet in order to control the level of specific minerals.
With the exception of sodium, it is necessary to use refined ingredients to achieve the lowest possible levels of minerals in a diet.
From this mineral deficient base, various levels of minerals can be added back. Some formulas adjust multiple minerals, and other related nutrients.
Contact us to discuss other possibilities for the minerals listed above, and for those minerals not listed here.
一些矿物调整公式如下所示。请与我们联系，以获得更多的这种性质的配方，或更多的信息，改变矿物质的饮食。有关更多信息，请阅读下面。
公式示例：
TD.80396缺铁饮食
TD.85419缺锌饮食
TD.88239缺钾饮食
TD.95027低钙饮食(0.4%P)
TD.96348日粮(20%乳糖，2%钙，1.25%P)
TD.92163缺硒饮食
TD.95125缺碘(0.15%PTU)
独特的蛋白质成分可能是必要的，以尽量减少某些矿物质的背景水平。为尽量减少背景矿物污染而采取的其他步骤包括在必要时使用试剂级矿物或遗漏某些成分。在这些饮食中可以使用特殊的矿物预混料，也可以在饮食中单独添加矿物质，以控制特定矿物质的含量。
除钠外，必须使用精制成分，以达到饮食中矿物质含量尽可能低的水平。
从这种矿物缺乏的基础，不同水平的矿物可以补充回来。一些配方调整多种矿物质和其他相关营养素。
上海金畔生物科技有限公司（www.jinpanbio.com）提供生命科学研究领域系列产品，包括生化试剂、诊断试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料；修饰性PEG（Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG）；Sigma、Amresco、TCI、MP bio生化试剂；WAKO日本和光纯药、日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA；Megazyme食品分析检测试剂盒、日本共立理化学；Research diets、Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料；Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基；免疫诊断试剂包括:Bethyl抗体；Biolegend流式抗体、Abcam、CST、Santa Cruz抗体；Roche、TOYOBO、NEB品牌的酶；中检所、TRC、药典USP、EP、Reagecon标准品；耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。
上海金畔生物科技有限公司
服务热线:18301939375   QQ：3258089810    3259632176
Email:  info@jinpanbio.com
官网：http://www.jinpanbio.com

Harlan TD.88137 高脂饲料 Adjusted Kcal Diet （42% from fat)
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。
Harlan TD.88137 高脂饲料 Adjusted Kcal Diet （42% from fat)
A staple of atherosclerosis research
More than 25 years ago, our nutritionists collaborated with researchers at Rockefeller University to develop a diet with features of a ‘Western Diet’ to characterize and enhance atherosclerosis development in their newly generated Apoe deficient mouse model. With over 200 unique users worldwide, TD.88137 continues to be fed to genetically modified cardiovascular models to accelerate and enhance hypercholesterolemia and plaque formation.
Formula                                             g/Kg
Casein                                              195.0
DL-Methionine                                     3.0
Sucrose                                            341.46
Corn Starch                                       150.0
Andydrous Milkfat                               210.0
Cholesterol                                          1.5
Cellulose                                              50.0
Mineral Mix, AIN-76 (170915)               35.0
Calcium Carbonate                               4.0
Vitamin Mix, Teklad (40060)                10.0
Ethoxyquin                                          0.04
Critical dietary features of TD.88137 related to atherosclerosis development include:
++Cholesterol (0.2% total cholesterol)
++Total fat (21% by weight; 42% kcal from fat)
++High in saturated fatty acids  (>60% of total fatty acids)
++High sucrose (34% by weight)
Typical fatty acid profile of TD.88137
Typical fatty acid analysis,% of diet1                   Mean                             SD
Total                                                                    20.7                                1.5
Saturated fat                                                       12.8                                0.8
Monounsaturated fat                                             5.6                                0.5
Polyunsaturated fat                                             1.0                                  0.2
Unknown2                                                           1.3                                   0.3
Selected nutrient information1
Key points from the literature
TD.88137 has been used to accelerate atherosclerosis development in Apoe and Ldlr deficient models:
++In Apoe deficient mice, plasma cholesterol triples to >1500 mg/dL within three weeks (1, 2). Foam cell and lesion development occurs within 6-10 weeks
(2-4). Fibrous plaque formation is observed at 15 weeks with the development of fibrous caps after 20 weeks (2).
++Ldlr deficient mice fed for two weeks increase plasma cholesterol to >800 mg/dL and triglyceride to >300 mg/dL (5). After six weeks of feeding, hyperglycemia, hyperinsulinemia and dyslipidemia develop with small foam cell lesions in the aortic
arch (6, 7).
With over 420 citations, uses of TD.88137 continue to evolve and include atherosclerosis, obesity, non-alcoholic steatohepatitis (NASH), osteoporosis,
hypertension and metabolic syndrome. Contact us for a more extensive reference list.
Control diet options for TD.88137
Natural ingredient diets
++Also referred to as standard diets or chow
++Diets differ in the source and level of nutrients as well as the presence of non-nutritive factors (such as phytates or phytoestrogens) compared
to TD.88137
++Limits inferences to differences in dietary pattern versus a specific dietary component
Ingredient matched, low fat diets
++Controls for the type of ingredients, non-nutritive components and the source and level of specific nutrients
++Suggested ingredient matched, low fat dietary controls for TD.88137 listed below; data sheets can be found on our website at envigo.com
Suggested ingredient matched, low fat controls

References  参考文献
1. Plump, A.S., et al., Severe hypercholesterolemia and atherosclerosis in
apolipoprotein E-deficient mice created by homologous recombination in
ES cells. Cell, 1992. 71(2): p. 343-53.
2. Nakashima, Y., et al., Apoe-deficient mice develop lesions of all phases of
atherosclerosis throughout the arterial tree. Arterioscler Thromb, 1994.
14(1): p. 133-40.
3. Febbraio, M., et al., Targeted disruption of the class B scavenger receptor
CD36 protects against atherosclerotic lesion development in mice.
J Clin Invest, 2000. 105(8): p. 1049-56.
4. Nakashima, Y., et al., Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone
sites on the endothelium in the Apoe-deficient mouse. Arterioscler Thromb
Vasc Biol, 1998. 18(5): p. 842-51.
5. Towler, D.A., et al., Diet-induced diabetes activates an osteogenic gene regulatory
program in the aortas of low density lipoprotein receptor-deficient mice.
J Biol Chem, 1998. 273(46): p. 30427-34.
6. Tsuchiya, K., et al., FoxOs integrate pleiotropic actions of insulin in vascular
endothelium to protect mice from atherosclerosis. Cell Metab, 2012. 15(3): p. 372-81.
7. Huszar, D., et al., Increased LDL cholesterol and atherosclerosis in LDL receptordeficient
mice with attenuated expression of scavenger receptor B1. Arterioscler
Thromb Vasc Biol, 2000. 20(4): p. 1068-73.
8. Yang, B., et al., Changes of skeletal muscle adiponectin content in diet-induced
insulin resistant rats. Biochem Biophys Res Commun, 2006. 341(1): p. 209-17.
9. Schafer, K., et al., Leptin promotes vascular remodeling and neointimal growth
in mice. Arterioscler Thromb Vasc Biol, 2004. 24(1): p. 112-7.
10. Lijnen, H.R., et al., Nutritionally induced obesity is attenuated in transgenic mice
overexpressing plasminogen activator inhibitor-1. Arterioscler Thromb Vasc
Biol, 2003. 23(1): p. 78-84.
11. Maquoi, E., et al., Modulation of adipose tissue expression of murine matrix
metalloproteinases and their tissue inhibitors with obesity. Diabetes, 2002.
51(4): p. 1093-101.
12. VanSaun MN, et al. 2009. High fat diet induced hepatic steatosis establishes a
permissive microenvironment for colorectal metastases and promotes primary
dysplasia in a murine model. Am J Pathol 175:355-64.
13. Dixon LJ, et al. 2013. Caspase-1 as a central regulator of high fat diet-induced
non-alcoholic steatohepatitis. PLoS One 8:e56100.

Harlan 维生素调整饲料 Vitamin adjusted diets
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。
A few vitamin adjusted formulas are shown below. Please contact us for additional formulas of this nature or for more information about altering the vitamin profile of a diet, and please read below to learn more.
Formula Examples:
TD.86143 Vitamin A deficient diet
TD.89123 Vitamin D deficient diet
TD.88163 Vitamin E deficient diet
TD.95247 Folic acid deficient diet
Most vitamin deficient diets use vitamin-free test casein (VFT casein) as the protein source. VFT casein is casein that has been extracted with denatured alcohol to reduce the fat content from about 1% to 0.1%. In the process the content of a number of vitamins (present at low or trace levels in casein) is further reduced, making this an ideal protein source for these types of diets. Envigo produces our own VFT in-house, and we also offer this as an ingredient for those mixing their own diets.
From this vitamin deficient base, various levels of vitamins can be added back. Many formulas adjust multiple vitamins, and other nutrients.
If use of an intact protein source is not advisable, an amino acid defined diet can be used.
一些维生素调整公式如下所示。请与我们联系，以获得更多的这种性质的配方或更多的信息，以改变维生素的饮食概况，并请阅读下面来了解更多。
公式示例：
TD.86143维生素A缺乏饮食
TD.89123维生素D缺乏饮食
TD.88163维生素E缺乏饮食
TD.95247叶酸缺乏饮食
大多数缺乏维生素的饮食使用无维生素的测试酪蛋白(VFT酪蛋白)作为蛋白质来源。VFT酪蛋白是用变性醇提取的酪蛋白，使脂肪含量从1%左右降至0.1%左右。在这个过程中，一些维生素的含量(在低水平或微量的酪蛋白)被进一步减少，使之成为这些类型的饮食的理想的蛋白质来源。Envigo生产我们自己的VFT内部产品，我们也为那些混合他们自己的饮食的人提供这种材料。
从这个缺乏维生素的基础，不同水平的维生素可以补充回来。许多配方调整多种维生素和其他营养。
如果使用完整的蛋白质源是不可取的，氨基酸限定饮食可以使用。
上海金畔生物科技有限公司（www.jinpanbio.com）提供生命科学研究领域系列产品，包括生化试剂、诊断试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料；修饰性PEG（Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG）；Sigma、Amresco、TCI、MP bio生化试剂；WAKO日本和光纯药、日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA；Megazyme食品分析检测试剂盒、日本共立理化学；Research diets、Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料；Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基；免疫诊断试剂包括:Bethyl抗体；Biolegend流式抗体、Abcam、CST、Santa Cruz抗体；Roche、TOYOBO、NEB品牌的酶；中检所、TRC、药典USP、EP、Reagecon标准品；耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。
上海金畔生物科技有限公司
服务热线:18301939375   QQ：3258089810    3259632176
Email:  info@jinpanbio.com
官网：http://www.jinpanbio.com

Harlan 动脉粥样硬化(胆固醇、脂肪、胆酸盐) 饲料Atherogenic
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。
Harlan 动脉粥样硬化(胆固醇、脂肪、胆酸盐) 饲料Atherogenic
Atherogenic

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis. However, if including a cholate source is not desired for your research, diets without cholate are available.
*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis.However, if including a cholate source is not desired for your research, diets without cholate are available.

“Western” purified atherogenic diet
“Western” style diets are fed to genetically-modified cardiovascular models, such as Apoe and Ldlr deficient mice, to accelerate and enhance hypercholesterolemia and plaque formation and to elicit phenotypes commonly associated with metabolic syndrome.Within the atherogenic literature, a “Western” diet typically is described as a purified rodent diet with 20-23% milkfat/butterfat, 0.2% total cholesterol, and 34% sucrose by weight.TD.88137is an example of a “Western” style diet that was originally designed to characterize and enhance atherosclerosis development in a newly generated Apoe-deficient mouse model.Contact us for more information about “Western” style diets, modifications, or possible control diets.
Examples:
TD.88137Adjusted calories diet (42% from fat, 0.2% total cholesterol)
TD.1088545% fat Kcal diet (0.2% total cholesterol)
Research use:
Accelerated hypercholesterolemia and plaque formation in genetically-modified models, such as Apoe and Ldlr deficient mice.
Used for diet-induced obesity in a variety of rodent models.
Key dietary features:
High Fat Diet (20-23% by weight; 40 – 45% kcal from fat)
Saturated fatty acids (SFA >60% of total fatty acids)
Milkfat/butterfat
Sucrose (34% by weight)
Cholesterol (0.2% total)
References:
1.Febbraio, M., et al., Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice.J Clin Invest, 2000.105(8): p.1049-56.
2.Huszar, D., et al., Increased LDL cholesterol and atherosclerosis in LDL receptor-deficient mice with attenuated expression of scavenger receptor B1.Arterioscler Thromb Vasc Biol, 2000.20(4): p.1068-73.
3.Nakashima, Y., et al., ApoE-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree.Arterioscler Thromb, 1994.14(1): p.133-40.
4.Nakashima, Y., et al., Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone sites on the endothelium in the ApoE-deficient mouse.Arterioscler Thromb Vasc Biol, 1998.18(5): p.842-51.
5.Plump, A.S., et al., Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells.Cell, 1992.71(2): p.343-53.
6.Towler, D.A., et al., Diet-induced diabetes activates an osteogenic gene regulatory program in the aortas of low density lipoprotein receptor-deficient mice.J Biol Chem, 1998.273(46): p.30427-34.
7,Tsuchiya, K., et al., FoxOs integrate pleiotropic actions of insulin in vascular endothelium to protect mice from atherosclerosis.Cell Metab, 2012.15(3): p.372-81.
“Western” purified atherogenic diet with added cholesterol and cholate source*
Wild type mice and rats generally are resistant to atherosclerosis, requiring more extreme dietary manipulation to modify lipoprotein profiles and develop mild atherosclerosis (foam cells, fatty streaks).Modern formulations are made completely of purified ingredients because this more refined approach has been reported to decrease the incidence of gallstones and liver damage associated with less refined and more traditional dietary approaches.To induce mild atherosclerosis in wild type animals, the “Western” purified diet can be modified to increase cholesterol (1-1.25%) and add a bile salt such as sodium cholate or cholic acid.Contact us for more information, modifications, or possible control diets.
Examples of purified high fat diets with added cholesterol and cholate source*:
TD.0202821% milkfat (1.25% cholesterol, 0.5% cholic acid)
TD.0923715% milkfat diet (1% cholesterol, 0.5% sodium cholate)
Examples of purified high fat diets with added cholesterol (without cholate source):
TD.9612121% milkfat (1.25% cholesterol)
Research Use:
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) primarily in wild type mice and rats.
Will not promote obesity.
Key dietary features:
High fat diet (15-20% by weight; 34 – 45% kcal from fat)
Saturated fatty acids (SFA >55% of total fatty acids)
Milkfat/butterfat, cocoa butter
Sucrose (30-50% by weight)
Cholesterol (1 – 1.25%)
Cholate source (0.5%)*
References:
1.Bernal, C., et al., Lipid biomarkers and metabolic effects of lycopene from tomato juice on liver of rats with induced hepatic steatosis.J Nutr Biochem, 2013.24(11): p.1870-81.
2.Gao, Q., et al., Atherogenic diets exacerbate colitis in mice deficient in glutathione peroxidase.Inflamm Bowel Dis, 2010.16(12): p.2043-54.
3.Lichtman, A.H., et al., Hyperlipidemia and atherosclerotic lesion development in LDL receptor-deficient mice fed defined semipurified diets with and without cholate.Arterioscler Thromb Vasc Biol, 1999.19(8): p.1938-44.
4.Marcondes, M.C., et al., Effects of chronic mental stress and atherogenic diet on the immune inflammatory environment in mouse aorta.Brain Behav Immun, 2011.25(8): p.1649-57.
5.Nishina, P.M., et al., Effects of dietary fats from animal and plant sources on diet-induced fatty streak lesions in C57BL/6J mice.J Lipid Res, 1993.34(8): p.1413-22.
6.Nishina, P.M., et al., Atherosclerosis and plasma and liver lipids in nine inbred strains of mice.Lipids, 1993.28(7): p.599-605.
7.Yue, P., et al., Enhanced hepatic apoA-I secretion and peripheral efflux of cholesterol and phospholipid in CD36 null mice.PLoS One, 2010.5(3): p.e9906.
8.Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse.J Lipid Res, 1990.31(5): p.859-69.
*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis.However, if including a cholate source is not desired for your research, diets without cholate are available.SeeTD.96121for a purified diet andTD.94059for a hybrid diet.Contact us for additional options.
Hybrid high fat diets with added cholesterol and cholate source*
Beverly Paigen and colleagues first characterized atherosclerosis development in C57BL/6 mice by feeding a hybrid atherogenic diet.The hybrid diet was created by mixing a natural ingredient mouse diet in a 3:1 ratio with a concentrated purified diet (containing 5% cholesterol and 2% sodium cholate; referred to as Thomas-Hartroft diet).The resulting mixture recreated inTD.88051/TD.90221(same formula) contains ~15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate.This group later compared the hybrid atherogenic diet approach to the more modern “western” purified atherogenic diet with added cholesterol and cholate and found that the hybrid atherogenic diet induced more gallstones and liver damage.Hybrid diets contain a variety of unrefined ingredients that may modify lipid metabolism and atherogenesis and do not allow for precise control of ingredients and nutrients for the study of chronic diseases.Although more refined diets have been developed, hybrid atherogenic diets are still popular for inducing mild atherosclerosis and gallstones in wild type mice and rats.Contact us for more information, modifications, or possible control diets.
Examples of hybrid high-fat diets with added cholesterol and cholate source*:
TD.88051andTD.90221(same formula) are Teklad product codes for hybrid atherogenic diets
Example of hybrid high-fat diet with added cholesterol (without cholate source):
TD.94059
Research Use:
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) primarily in wild type mice and rats.
Will not promote obesity.
Also used for lithogenic (gallstone) rodent studies.
Key dietary features:
75% rodent breeder diet; 25% purified ingredients
High fat (~15% by weight; 37% kcal from fat)
Saturated fatty acids (SFA >45% of total fatty acids)
Cholesterol (1.25%)
Cholate source (0.5%)*
References:
1.Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse.J Lipid Res, 1990.31(5): p.859-69.
2.Clee, S.M., et al., Plasma and vessel wall lipoprotein lipase have different roles in atherosclerosis.J Lipid Res, 2000.41(4): p.521-31.
3.George, J., et al., Enhanced fatty streak formation in C57BL/6J mice by immunization with heat shock protein-65.Arterioscler Thromb Vasc Biol, 1999.19(3): p.505-10.
4.Miyake, J.H., et al., Transgenic expression of cholesterol-7-alpha-hydroxylase prevents atherosclerosis in C57BL/6J mice.Arterioscler Thromb Vasc Biol, 2002.22(1): p.121-6.
5.Paigen, B., et al., Quantitative assessment of atherosclerotic lesions in mice.Atherosclerosis, 1987.68(3): p.231-40.
6.Schreyer, S.A., D.L. Wilson, and R.C. LeBoeuf, C57BL/6 mice fed high fat diets as models for diabetes-accelerated atherosclerosis.Atherosclerosis, 1998.136(1): p.17-24.
7.Vergnes, L., et al., Cholesterol and cholate components of an atherogenic diet induce distinct stages of hepatic inflammatory gene expression.J Biol Chem, 2003.278(44): p.42774-84.
*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis.However, if including a cholate source is not desired for your research, diets without cholate are available.SeeTD.96121for a purified diet andTD.94059for a hybrid diet.Contact us for additional options.
Standard diets with added cholesterol
Standard, natural ingredient diets with cholesterol added are fed to induce hypercholesterolemia.Various levels of cholesterol, fat, and/or bile salts can be added to one of the numerous standard rodent diets stocked by Envigo Teklad.For many applications, adding these components to Envigo’s minimal-to-moderate phytoestrogen global rodent diets is recommended.Our minimal phytoestrogen global rodent diets are soybean meal free, limiting the effect of phytoestrogens on your research outcomes.Soybean meal, a common dietary source of phytoestrogens, has been shown to decrease aortic fatty streak development and modify plasma cholesterol, which may reduce the risk of developing atherosclerosis.Limiting dietary soybean meal may reduce confounding variables within your dietary-induced atherosclerosis model.Contact a nutritionist to discuss additional diet options.
Examples of minimal and moderate phytoestrogen rodent diets with added cholesterol:
TD.1200971% cholesterol diet (2020 – minimal phytoestrogens)
TD.078412% cholesterol diet (2016 – minimal phytoestrogens)
TD.013832% cholesterol (2018 – Moderate phytoestrogens)
Research use:
Induce hypercholesterolemia in genetically-modified and wild type models without promoting obesity.
Key dietary features:
Standard, grain-based rodent diet
Minimal/moderate phytoestrogen diets recommended
Cholesterol (1 – 4%)
References:
1.Belch, J.J., et al., Longitudinal assessment of endothelial function in the microvasculature of mice in-vivo.Microvasc Res, 2013.85: p.86-92.
2.Hartvigsen, K., et al., A diet-induced hypercholesterolemic murine model to study atherogenesis without obesity and metabolic syndrome.Arterioscler Thromb Vasc Biol, 2007.27(4): p.878-85.
Diets for additional animal models of atherosclerosis
Rabbits, hamsters, and swine are common models of atherosclerosis.Contact a nutritionist for information and formula examples.Seerabbit, swine and other speciesfor information and formula examples.
“西方”纯正致动脉粥样硬化饮食

Harlan MCD饲料 TD.90262 Methionine/Choline Deficient Diet
品牌：Harlan Teklad
货号：TD.90262
英文名称：Methionine/Choline Deficient Diet
别名：MCD饲料
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Harlan MCD饲料 TD.90262 Methionine/Choline Deficient Diet
 

Harlan蛋白质调节饲料 Protein adjusted diets
Teklad diet, bedding and enrichment + Teklad laboratory animal diets + Custom research diets + Protein adjusted diets
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。
Harlan蛋白质调节饲料 Protein adjusted diets
Protein adjusted diets | Envigo
A few protein adjusted formulas are shown below. Please contact us for additional formulas of this nature or for more information about altering the protein type or content of a diet.
Formula examples:
TD.93328      “Protein free” diet
TD.90016      6% Protein diet
TD.91352       20% Protein diet
TD.90018      40% Protein diet
These diet examples are from a series of diets with the following features: casein-based, isocaloric (3.8 kcal/g) and matched for fat (5.5%), calcium (0.7%), and phosphorus (0.54%).  There are other diets available from this series of diets, as well as other unique protein adjusted diets.
Casein is the most widely used refined protein source, but it is also possible to use other refined protein sources, such as “vitamin-free” test casein (alcohol extracted), lactalbumin, isolated soy protein, and egg white solids. These particular protein sources are usually used to achieve a specific research objective, rather than for routine use. For instance, vitamin-free test casein is most appropriately used in vitamin deficient diets, and egg white solids is most suitable for zinc or biotin deficient diets.
Adjusted protein diets usually range from “protein-free” (trace) to 60% protein, depending on the research.
一些蛋白质调整的公式如下所示。请与我们联系，以获得更多的这种性质的配方，或更多有关改变蛋白质类型或饮食内容的信息。
公式示例：
TD.93328“无蛋白质”饮食
TD.90016  6%蛋白质饮食
TD.91352  20%蛋白质饮食
TD.90018  40%蛋白质饮食
这些饮食的例子来自一系列的饮食，有以下特点：酪蛋白为基础，等热量(3.8千卡/克)，与脂肪(5.5%)，钙(0.7%)和磷(0.54%)匹配。从这一系列的饮食中可以得到其他的饮食，以及其他独特的蛋白质调整的饮食。
酪蛋白是最广泛使用的精制蛋白质来源，但也可以使用其他精制蛋白质来源，如“无维生素”测试酪蛋白(醇提)，乳清蛋白，分离大豆蛋白和蛋清固体。这些特定的蛋白质来源通常用于实现特定的研究目标，而不是用于常规用途。例如，无维生素测试酪蛋白最适合用于缺乏维生素的饮食，而蛋清固体最适合于锌或生物素缺乏的饮食。
根据研究结果，调整后的蛋白质饮食通常从“无蛋白质”(微量)到60%的蛋白质。
上海金畔生物科技有限公司（www.jinpanbio.com）提供生命科学研究领域系列产品，包括生化试剂、诊断试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料；修饰性PEG（Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG）；Sigma、Amresco、TCI、MP bio生化试剂；WAKO日本和光纯药、日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA；Megazyme食品分析检测试剂盒、日本共立理化学；Research diets、Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料；Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基；免疫诊断试剂包括:Bethyl抗体；Biolegend流式抗体、Abcam、CST、Santa Cruz抗体；Roche、TOYOBO、NEB品牌的酶；中检所、TRC、药典USP、EP、Reagecon标准品；耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。
上海金畔生物科技有限公司
服务热线:18301939375   QQ：3258089810    3259632176
Email:  info@jinpanbio.com
官网：http://www.jinpanbio.com