分类目录归档：Megazyme

D-木糖检测试剂盒 D-Xylose assay Kit

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D-木糖检测试剂盒 D-Xylose assay Kit

产品名称：D-木糖检测试剂盒
英文名称：D-xylose including xylan and arabinoxylan assay kit
货号： K-XYLOSE
规格：80 assays (manual) / 800 assays (microplate)
优点：成本低；所有试剂配制后的稳定性〉2年；只有酶试剂盒可用；反应快；包含标准品

检测原理：

检测方法：紫外分光光度法

检测波长：340nm；

反应时间：30min；

检测限：2.96mg/L；

适用样品：发酵液、植物原料和多聚糖产物中的木糖的分析；

方法认证：新方法

商品说明：
The D-Xylose test kit is a novel method for the specific, convenient and rapid measurement and analysis of D-xylose in plant extracts, culture media/supernatants and other materials.
Suitable for manual, auto-analyser and microplate formats.
UV-method for the determination of D-Xylose in fermentation
broths and hydrolysates of plant material and polysaccharides
Principle:
(xylose mutarotase)
(1) α-D-Xylose ↔ β-D-xylose
(β-xylose dehydrogenase)
(2) β-D-Xylose + NAD+ → D-xylonic acid + NADH + H+
Kit size: 100 assays (manual) / 1000 (microplate)
/ 1300 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 6 min
Detection limit: 0.7 mg/L
Application examples:
Analysis of D-xylose in fermentation broths and hydrolysates of plant
material and polysaccharides
Method recognition: Novel method
Advantages

  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Rapid reaction (~ 6 min)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

L-苹果酸检测试剂盒

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L-苹果酸检测试剂盒L-Malic Acid Assay Kit (Manual Format)

产品货号： K-LMALL
产品品名： L- 苹果酸检测试剂盒（大）
英文品名： L-Malic Acid Assay Kit (Large)
规格型号： 116 assays
原理：
反应时间： 3min
检测限： 0.25 mg/L,
适用样品： 饮料和食品以及其他原料
优点：
l    成本低
l    使用期间所有试剂的稳定性 >2 年
l    操作简单
l    包含标准品
REFERENCES:

  1. Mollering, H. (1985). L-Malate. In  Methods of Enzymatic Analysis  (Bergmeyer, H. U., ed.), 3rd ed., Vol. VII, pp. 39-47, VCH Publishers (UK) Ltd., Cambridge, UK.
  2. AOAC Official Methods of Analysis (2002). Method 993.05 “L-Malic acid/total malic acid ratio in apple juice”. 17th ed. Chapter 37, p. 15.
  3. Gorin, N. (1976). Differences in L-malate determined enzymatically or titrimetrically in golden delicious apples.  Zeitschrift fur Lebensmittel-Unterschung und-Forschung,  162, 259-261.
  4. Klopper, W. J., Angelino, S. A. G. F., Tuning, B. & Vermeire, H. A. (1986). Organic acids and glycerol in beer.  J. Inst. Brew.  92, 225-228.
  5. Elkins, E. R. & Freund, W. (1994). Detection of adulteration in apple juice by L-malic/total malic acid ratio: Collaborative Study.  J. AOAC Int . 77, 411-415.
商品说明：
L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.
Manual format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials
Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+
(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate
Kit size: (K-LMALR)
58 assays (manual) / 580 (microplate) or
(K-LMALL)
116 assays (manual) / 1160 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK
Advantages

  • PVP incorporated to prevent tannin inhibition
  • Both enzymes supplied as stable suspensions
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction (~ 3 min)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual and microplate format

α-淀粉酶/液化酶/淀粉糖化酶检测试剂盒

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α-淀粉酶/液化酶/淀粉糖化酶检测试剂盒

产品货号：K-CERA
产品品名：α-淀粉酶检测试剂盒
英文品名：Ceralpha: α-Amylase Assay Kit
规格型号：100 assays per kit
α-淀粉酶能水解淀粉中的α-1,4-葡萄糖苷键。能将淀粉切断成长短不一的短链糊精和少量的低分子糖类，从而使淀粉糊的黏度迅速下降，即起到降低稠度和“液化”的作用，所以此类淀粉酶又称为液化酶。
上海西宝生物提供原装进口：α-淀粉酶检测试剂盒，采用比色法（400nm）测定食品、饲料和发酵产品中a -淀粉酶，咨询热线：400-021-8158
原理：
反应时间：20min
检测限：0.05U/mL
适用样品：谷物粉，发酵液和其他原料
优点：
  成本低
所有试剂配制后的稳定性>2年
特异性高
方法简单
包含标准品
认证方法：
AOAC（方法2002. 01），AACC（方法22. 02），ICC (标准号303)，RACI（标准方法）和CCFRA(面粉检测工作组方法0018)
REFERENCES:
1. McCleary, B. V. & Sheehan, H. (1987). Measurement of Cereal α-Amylase: A New Assay Procedure. Journal of Cereal Science, 6, 237-251.
2. Sheehan, H. & McCleary, B. V. (1988). A New Procedure for the Measurement of Fungal and Bacterial α?Amylase.Biotechnology Techniques, 2, 289-292.
3. McCleary, B. V., McNally, M., Monaghan, D. & Mugford, D. C. (2002). Measurement of α-amylase activity in white wheat flour, milled malt, and microbial enzyme preparations using the Ceralpha Assay collaborative study. J. AOAC International, 85, 1096-1102.