乳果糖快速检测方法—Megazyme乳果糖快速检测试剂盒
Megazyme乳果糖检测试剂盒采用酶法分析原理，可定量检测鲜奶、UHT奶、浓缩乳和奶粉等各种乳品中的乳果糖。
与农业部新标准采用相同的检测原理和方法
该试剂盒采用ISO方法11285：2004，经过改进后，更加快速和灵敏
灵敏度是传统己糖激酶法的两倍
成本低
试剂配制后可稳定保存2年
采用试剂盒形式，包含检测必需的所有酶
提供计算软件，使数据处理更方便
包含标准品
The Lactulose Assay Kit is suitable for the specific, rapid and sensitive measurement and analysis of lactulose in milk and milk-based samples. Reagents included in this kit may also be prepared for use in the procedure described by ISO Method 11285:2004.
UV-method for the determination of Lactulose in milk and
foodstuffs containing dairy products
Principle:
(β-galactosidase)
(1) Lactulose + H₂O → D-galactose + D-fructose
(glucose oxidase + catalase + H₂O₂)
(2) D-Glucose + H₂O + O₂ → D-gluconic acid + H₂O₂
(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP
(phosphoglucose isomerase)
(4) F-6-P → G-6-P
(glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺
(gluconate-6-phosphate dehydrogenase)
(6) Gluconate-6-phosphate + NADP⁺ → ribulose-5-phosphate + NADPH
+ CO₂ + H⁺
Kit size: 50 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 120 min
Detection limit: 4.8 mg/L
Application examples:
Milk, dairy products and foods containing milk
Method recognition: Novel method
Advantages

• Twice the sensitivity of traditional hexokinase based lactulose methods
• Very cost effective
• All reagents stable for > 2 years after preparation
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Standard included

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