Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit酶试剂盒

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iPS Cell to Endothelial Cell Differentiation System
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit ￥13,273 Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit
Cellartis Y50301 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit with DEF-CS™ 500 Culture System 1 Kit ￥18,627 Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit
Cellartis Y50053 MiraCell® EC Culture Medium 500 ml ￥4,148 Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit
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iPS向血管内皮细胞定向分化系统
(iPS Cell to Endothelial Cell Differentiation System)
MiraCell iPS Cell to Endothelial Cell Differentiation Kit用于人iPS细胞向血管内皮细胞定向分化诱导，试剂盒中包含2种包被剂、2种分化用基础培养基和6种培养基添加剂，足够支持1个T25培养瓶 (1.5×105 Cells）或3个6孔板单孔（1.8×105 cells）的iPS细胞进行内皮方向分化诱导，分化周期为12天（Day0至Day11）。建议分化起始的iPS细胞先使用Cellartis® DEF-CS™ 500 Culture System（Y30010）驯化3-5代，以确保iPS细胞处于良好的多能性状态。
诱导分化获得的高纯度（CD31阳性率＞80％＊）血管内皮细胞，经鉴定表达CD31、CD144、Tie2、VEGFR2等血管内皮标志物和具备小管（Tube）形成能力。分化好的血管内皮细胞(Day12以后)可以使用MiraCell EC Culture Medium(Y50053)长期扩大培养，也可以冷冻保存备用。（*起始的人iPS细胞株不同，得到的血管内皮细胞性能可能不同。）
 
本产品使用京都大学iPS细胞研究所开发的人血管内皮细胞的制备技术，由Takara和iHeart Japan公司共同研发，由Takara制造并销售。
 
■ 产品特点
· 完整的试剂盒，支持人iPS细胞-中胚层细胞-血管内皮细胞定向分化
· 多个iPS细胞株测试验证，支持高效分化诱导(>80%)
· 无需基因修饰和药物筛选过程，操作简便
· 获得的血管内皮细胞，可冷冻保存和扩大培养
 
■ 产品组成
MiraCell® iPS Cell to Endothelial Cell Differentiation Kit，Y50300
· Coating Reagent 1 (Day0 ＆ 2)；Coating Reagent 2 (Day11)
· EC Differentiation Basal Medium 1 (Day3～9)；EC Differentiation Basal Medium 2 (Day11)
· Supplement 1 (Day3)；Supplement 2 (Day4)；Supplement 3 (Day7)；Supplement 4 (Day8)；Supplement 5 (Day9)；Supplement 6 (Day11)
 
Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit
 
MiraCell® iPS Cell to Endothelial Cell Differentiation Kit with DEF-CS™ 500 Culture System，Y50301
· MiraCell® iPS Cell to Endothelial Cell Differentiation Kit，Y50300
· Cellartis® DEF-CS™ 500 Culture System，Y30010
 
Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit
 
■ 实验例
Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit
图1. 3种人iPS细胞株使用MiraCell iPS Cell to Endothelial Cell Differentiation Kit分化诱导，获得的血管内皮细胞的形态比较(A)和标志蛋白质(B)比较。
 
Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit
图2. 3种人iPS细胞株使用MiraCell iPS Cell to Endothelial Cell Differentiation Kit分化诱导，获得的血管内皮细胞继续使用MiraCell EC Culture Medium维持培养，可长时间维持高纯度。（）内的数值表示Day32的细胞扩大倍数，以Day8的细胞数为1。
 
Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit
图3. 冷冻保存的、使用MiraCell iPS Cell to Endothelial Cell Differentiation Kit分化诱导的血管内皮细胞（Day16），在解冻复苏培养Day8后的小管形成实验结果。
 
 
 
产品详情请点击：http://catalog.takara-bio.co.jp/product/basic_info.php?unitid=U100009383