The Cellartis Definitive Endoderm Differentiation Kit用于定向分化hiPS获取definitive endoderm (DE) cells。本产品包含所有必需的培养基和coating材料。本产品还包括新一代的Cellartis DEF-CS 100 Culture System用于起始hiPS的维持培养用。使用本产品获得的DE细胞,可以作为起始材料进行定向分化至内胚层来源的细胞类型(如肝细胞,胰腺细胞)。Cellartis也提供分化DE细胞获取肝细胞的试剂盒(Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050);Cellartis也提供诱导分化hiPS获取肝细胞的试剂盒(Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)。
■ 产品特点
· Efficient differentiation—generates >80% SOX17-positive cells · Robust protocol—successful definitive endoderm (DE) cell generation from more than 25 different hiPS cell lines · Complete kit—media and ready-to-use coating for a seven-day protocol to reproducibly differentiate hiPS cells into DE cells · Start with a homogeneous population of hiPS cells—kit includes the Cellartis DEF-CS 100 Culture System to maintain undifferentiated cells prior to the start of DE cell differentiation
■ 产品详情请点击:
mRNA expression profiles of definitive endoderm cells being generated from two hiPS cell lines (ChiPSC18 and P11025) using the Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture Medium. These graphs track the relative temporal mRNA expression levels of various markers during differentiation from hiPS cells (starting on Day 0) into DE cells (by Day 7), as measured by RT-qPCR.