人iPS细胞培养系统酶试剂盒Takara Clontech

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人iPS细胞培养系统
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Cellartis Y30012 Cellartis® DEF-CS™ 500 COAT-1 4 ml 询价 人iPS细胞培养系统 人iPS细胞培养系统 人iPS细胞培养系统
Cellartis Y30010 DEF-CS™ 500 1 Kit ￥5,979 人iPS细胞培养系统 人iPS细胞培养系统 人iPS细胞培养系统
Cellartis Y30016 Cellartis® DEF-CS™ 500 Additives 1 set 询价 人iPS细胞培养系统 人iPS细胞培养系统 人iPS细胞培养系统
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人iPS细胞培养系统酶试剂盒Takara Clontech – 修饰性PEG

Human iPS和Human ES增殖用培养基
(DEF-CS Culture System, all-in-one format)
作为一款高端创新型的产品，Cellartis DEF-CS培养系统使得单细胞干细胞操作成为常规的操作。该产品是专门针对人类诱导干细胞（hiPS）和人类胚胎干细胞（hES）研发的高效率增殖用培养基产品体系。该产品系统为all-in-one型，包括了所有组分，客户不需要另外求购组分。该产品是成分确定的培养基产品，而且不需要饲养层。DEF-CS既可以实现单细胞培养，也可以用于传统的iPS培养模式以及大规模干细胞增殖。在使用DEF-CS培养基增殖干细胞时，几乎没有背景分化的问题，这使得细胞筛选不再必需。作为一款创新产品，利用DEF-CS培养干细胞时，可以使用酶消化法（enzymatic passaging）实现需要单细胞操作，这一特点十分有利于高通量细胞鉴别筛选（high-throughput screening）、转染（transfection）、框架接种（scaffold seeding）等。
 
■ 产品特点
· Feeder-free and chemically defined culture system for efficient expansion of human iPS cells
· Ideal system for culturing iPS cells; cells maintain pluripotency and long-term potential for self-renewal and differentiation
· Complete kit includes 500 ml basal medium, additives, and coating compound
· Robust system provides high reproducibility and stable growth
· Maintains cells in an undifferentiated state with virtually no background differentiation
 
■ 产品应用
· Scale-up and mass production of human iPS cells
· Single-cell culture of human iPS cells
· Transfection and reprogramming
· High-throughput screening
· Tissue engineering (seeding cells on a scaffold)
 
■ 产品详情请点击：人iPS细胞培养系统
 
人iPS细胞培养系统
Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System. The number of iPS cells was quantified after being cultured for three weeks using either the Cellartis DEF-CS Culture System, a reference feeder system, or four other stem cell culture systems.
 
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6. Borestrom, Cecilia, et al. “Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: A first step toward a clinical-grade cell source.” Stem Cells Trans. Med. (2014) 3, 433-447.
7. Kia, Richard, et al. "MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity." Toxicological Sciences (2014): kfu269.
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10. Asplund, Annika, et al. “One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying? Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells” Stem Cell Rev and Rep (2015)