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NEBNext RNA 第一链合成模块 收藏
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概述
The NEBNext RNA First Strand Synthesis Module is designed for synthesis of cDNA from RNA fragments using ProtoScript® II Reverse Transcriptase and random priming. Single strand cDNA can be used directly for second strand cDNA synthesis. For use in directional (strand-specific) protocols, Actinomycin D is
required and must be purchased separately.
NEBNext RNA 第一链合成模块组份
– NEBNext First Strand Synthesis Reaction Buffer
– Random Primers
– ProtoScript® II Reverse Transcriptase
– Murine RNase Inhibitor
注意事项
Materials Required but not Supplied
If you are using the module for Directional RNA Seq, Actinomycin D (Sigma #A1410 dissolved in
dimethylsulfoxide [DMSO] to 5μg/μl) is required. See protocol for details.
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