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Amplite ADHP过氧化物和过氧化酶荧光探针 相当于Amplex Red
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货号 | 11000 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 25 mg | 价格 | 2604 | |
| Ex (nm) | 571 | Em (nm) | 584 | |
| 分子量 | 257.24 | 溶剂 | DMSO | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：11000
产品名称：Amplite ADHP过氧化物和过氧化酶荧光探针
规格：25mg
储存条件：-15℃避光防潮
保质期：24个月
产品物理化学光谱特性
分子量：257.24
外观：白色固体
溶剂：DMSO
激发波长（nm）：571
发射波长（nm）：585
产品介绍
Amplite ADHP过氧化物和过氧化酶荧光探针是美国AAT Bioquest生产的过氧化氢酶荧光底物，Amplite ADHP与AmplexRed 在化学上是相同的（AmplexRed 是Invitrogen的商标）。它是一种敏感的荧光过氧化物酶底物，其背景比其他商业供应商的材料低得多。ADHP产生高荧光的试卤灵，其最大吸收为571nm，最大发射为585nm。与其他HRP底物如二氢荧光素和二氢罗丹明不同，ADHP的空气氧化是最小的。到目前为止，ADHP被认为是用于检测HRP和H2O2的最灵敏和最稳定的荧光探针。ADHP已被广泛用于检测许多免疫测定中的HRP。另一方面，ADHP也可用于检测痕量的H2O2。基于ADHP的H2O2检测比常用的硫氧还蛋白对H2O2的检测灵敏度至少高一个数量级。由于H2O2是在许多酶促氧化还原反应中产生的，因此ADHP可用于偶联酶促反应，以检测许多氧化酶和/或相关酶/底物或辅助因子如葡萄糖，乙酰胆碱和胆固醇，L-谷氨酸，氨基酸等的活性。简单地制备10-20mM DMSO储备溶液，然后稀释到测定缓冲液如PBS，Tris缓冲液中，用于测定50-200μM。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Amplite ADHP过氧化物和过氧化酶荧光探针。
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