Screen Quest 活细胞cAMP检测

	货号	36382	存储条件	在零下15度以下保存, 避免光照
	规格	100 Tests	价格	17748
	Ex (nm)	490	Em (nm)	520
	分子量		溶剂
	产品详细介绍

简要概述

产品基本信息

货号：36382

产品名称：Screen Quest 活细胞cAMP检测

规格：100 Tests

产品介绍

G蛋白偶联受体（GPCR）是药物发现计划中最大的靶向受体之一。钙通量（通过Gq途径耦合）分析是筛选GPCR靶点的首选方法。然而，超过60%的已知GPCRs信号通过腺苷酸环化酶活性偶联到cAMP。现有的cAMP检测方法不仅需要细胞裂解，而且缺乏时间和空间分辨率。Screen Quest 活细胞cAMP检测以高通量的形式提供细胞内cAMP变化的实时监测，无需细胞裂解步骤。该方法通过含有外源性环核苷酸门控通道（CNGC）或混杂G蛋白Gα16的细胞系进行。该通道被细胞内cAMP水平升高激活，导致离子通量和细胞膜去极化，可通过荧光钙（如Calbryte 520 AM、Cal-520 AM、Fluo-8 AM或Fluo-4 AM和相应的免洗钙试剂盒）或荧光膜电位染料检测到。Gα16与特异性非Gq偶联受体的共表达将在受体刺激下产生细胞内钙信号。Screen Quest 活细胞cAMP检测提供细胞系和试剂，用于使用FLIPR、FDSS或其他等效的荧光酶标仪检测细胞内cAMP的变化。它已成功地用于测定Gs和Gi偶联GPCR活性。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Screen Quest 活细胞cAMP检测。 

图示

图1. Screen Quest 活细胞cAMP测定原理

参考文献

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