生物素酪胺 CAS 41994-02-9

	货号	45350	存储条件	在零下15度以下保存, 避免光照
	规格	5 mg	价格	1200
	Ex (nm)		Em (nm)
	分子量	363.48	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：45350

产品名称：生物素酪胺

CAS：41994-02-9

规格：5mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：363.48

溶剂：DMSO

产品介绍

对于许多免疫组织化学（IHC）的应用，传统的酶扩增程序足以实现充分的抗原检测。然而，有几个因素限制了这些程序的敏感性和实用性。酪胺信号放大技术（TSA）已被证明是一种特别通用和强大的酶放大技术，提高了检测灵敏度。TSA是基于HRP在低浓度过氧化氢存在下将标记的酪胺底物转化为氧化的、高活性的自由基的能力，该自由基可在HRP处或其附近共价结合酪氨酸残基。这一过程被称为催化报告沉积（CARD），通过每个过氧化物酶标签上的多个酪胺底物的转换所产生的信号放大转化为对低丰度目标的超灵敏检测和使用少量抗体和杂交探针。在免疫组化应用中，TSA法的敏感性增强使原代抗体稀释度增加，以减少非特异性背景信号，并能克服由于固定程序不理想或靶蛋白表达水平低而导致的弱免疫标记。生物素酪胺被认为能在所有TSA试剂中实现最大限度的IHC检测。生物素酪胺已用于免疫组织化学、ELISA、Western blot和原位杂交应用。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的生物素酪胺。

参考文献

High specificity and tight spatial restriction of self-biotinylation by DNA and RNA G-Quadruplexes complexed in vitro and in vivo with Heme.
Authors: Lat, Prince Kumar and Liu, Kun and Kumar, Dev N and Wong, Kenneth K L and Verheyen, Esther M and Sen, Dipankar
Journal: Nucleic acids research (2020)

APEX Peroxidase-Catalyzed Proximity Labeling and Multiplexed Quantitative Proteomics.
Authors: Kalocsay, Marian
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 41-55

Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity.
Authors: Li, Wei and Zeng, Weiwu and Chen, Yi and Wang, Fang and Wu, Fan and Weng, Xiaocheng and Zhou, Xiang
Journal: The Analyst (2019): 4472-4476

Proximity labeling of cis-ligands of CD22/Siglec-2 reveals stepwise α2,6 sialic acid-dependent and -independent interactions.
Authors: Alborzian Deh Sheikh, Amin and Akatsu, Chizuru and Imamura, Akihiro and Abdu-Allah, Hajjaj H M and Takematsu, Hiromu and Ando, Hiromune and Ishida, Hideharu and Tsubata, Takeshi
Journal: Biochemical and biophysical research communications (2018): 854-859

Identification of Siglec Ligands Using a Proximity Labeling Method.
Authors: Chang, Lanyi and Chen, Yi-Ju and Fan, Chan-Yo and Tang, Chin-Ju and Chen, Yi-Hsiu and Low, Penk-Yeir and Ventura, Albert and Lin, Chun-Cheng and Chen, Yu-Ju and Angata, Takashi
Journal: Journal of proteome research (2017): 3929-3941

Tyramide Signal Amplification Permits Immunohistochemical Analyses of Androgen Receptors in the Rat Prefrontal Cortex.
Authors: Low, Katelyn L and Ma, Chunqi and Soma, Kiran K
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2017): 295-308

Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis.
Authors: Royston, Daniel J and Clasper, Steven and Jackson, David G
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 35-48

Development of a highly sensitive immunohistochemical method to detect neurochemical molecules in formalin-fixed and paraffin-embedded tissues from autopsied human brains.
Authors: Goto, Satoshi and Morigaki, Ryoma and Okita, Shinya and Nagahiro, Shinji and Kaji, Ryuji
Journal: Frontiers in neuroanatomy (2015): 22

Development of a sensitive ELISA for quantification of three- and four-repeat tau isoforms in tauopathies.
Authors: Luk, Connie and Giovannoni, Gavin and Williams, David R and Lees, Andrew J and de Silva, Rohan
Journal: Journal of neuroscience methods (2009): 34-42

Levels of pregnancy-associated plasma protein A in patients with coronary artery disease.
Authors: Liu, Zhi-Yuan and Zhang, Jin-Ying and Sun, Tong-Wen and Zhang, Yan-Jun and Zhang, Li and Wang, Le-Xin
Journal: Clinical and investigative medicine. Medecine clinique et experimentale (2008): E85-9