产品基本信息

货号：6900

产品名称：小沟结合物探针CDPI3-CPG*500A*

规格：100 mg

储存条件：-20℃干燥

保质期：12个月

产品物理化学光谱特性

外观：固体

溶剂：MeCN

产品介绍

小沟结合物（MGB）分子选择性地结合到DNA分子的小沟，即DNA螺旋中的浅沟。MGB标记的寡核苷酸与互补的DNA和RNA靶序列形成稳定的杂合复合物。天然产物CC-1065的类似物二氢吡咯并吲哚三肽（CDPI3）已表现出与寡核苷酸连接时可在单链DNA上阻止序列特异性引物延伸。包含CDPI3的寡核苷酸可提高特异性和杂交强度，从而增强DNA双链体的稳定性。将MGB分子添加到DNA探针可以设计出较短的杂交探针，该探针可用于分子诊断，例如基于定量PCR的测定。可能有几种设计，包括Taqman MGB探针。AAT Bioquest提供此CPG支持，可将MGB部分方便地添加到寡核苷酸的3’末端。我们还提供MGB亚磷酰胺，用于用MGB部分标记5’末端。

参考文献

Capecitabine as a minor groove binder of DNA: molecular docking, molecular dynamics, and multi-spectroscopic studies.
Authors: Zhang, Shuangshuang and Yang, Hongqin and Zhao, Ludan and Gan, Ruixue and Tang, Peixiao and Sun, Qiaomei and Xiong, Xinnuo and Li, Hui
Journal: Journal of biomolecular structure & dynamics (2019): 1451-1463

Minor groove binder modification of widely used TaqMan hydrolysis probe for detection of dengue virus reduces risk of false-negative real-time PCR results for serotype 4.
Authors: Gray, Eleanor R and Heaney, Judith and Ferns, R Bridget and Sequeira, Patricia C and Nastouli, Eleni and Garson, Jeremy A
Journal: Journal of virological methods (2019): 17-23

A rapid minor groove binder PCR method for distinguishing the vaccine strain Brucella abortus 104M.
Authors: Nan, Wenlong and Qin, Lide and Wang, Yong and Zhang, Yueyong and Tan, Pengfei and Chen, Yuqi and Mao, Kairong and Chen, Yiping
Journal: BMC veterinary research (2018): 27

Influence of Divalent Counterions on the Dynamics in DNA as Probed by Using a Minor-Groove Binder.
Authors: Paul, Sneha and Ahmed, Tasnim and Samanta, Anunay
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2017): 2058-2064

Origin of DNA-Induced Circular Dichroism in a Minor-Groove Binder.
Authors: Holmgaard List, Nanna and Knoops, Jérémie and Rubio-Magnieto, Jenifer and Idé, Julien and Beljonne, David and Norman, Patrick and Surin, Mathieu and Linares, Mathieu
Journal: Journal of the American Chemical Society (2017): 14947-14953

Development of a real-time quantitative PCR assay using a TaqMan minor groove binder probe for the detection of α-lactalbumin in food.
Authors: Xiao, Guan and Qin, Cai and Wenju, Zhang and Qin, Chen
Journal: Journal of dairy science (2016): 1716-1724

Grandivittin as a natural minor groove binder extracted from Ferulago macrocarpa to ct-DNA, experimental and in silico analysis.
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Journal: Chemico-biological interactions (2016): 89-101

Synthesis of a novel 4H-pyran analog as minor groove binder to DNA using ethidium bromide as fluorescence probe.
Authors: Ramana, M M V and Betkar, Rahul and Nimkar, Amey and Ranade, Prasanna and Mundhe, Balaji and Pardeshi, Sachin
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2016): 165-71

Dacarbazine as a minor groove binder of DNA: Spectroscopic, biophysical and molecular docking studies.
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Journal: International journal of biological macromolecules (2015): 193-200

Demonstration of the Pre-Emergency Use Authorization Path Using 3 Minor Groove Binder-Hydrolysis Probe Assays to Detect Escherichia coli O104:H4.
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Journal: Clinical chemistry (2015): 1391-8