Cell Meter 荧光法细胞电位检测试剂盒

	货号	35000	存储条件	Multiple
	规格	100 Tests	价格	8772
	Ex (nm)		Em (nm)
	分子量		溶剂
	产品详细介绍

简要概述

产品基本信息

货号：35000

产品名称：Cell Meter 荧光法细胞电位检测试剂盒

规格：100 Tests

保质期：12个月

产品介绍

几乎所有质膜在其上都具有电势，内部通常相对于外部为负。信号是通过打开或关闭膜上某一点的离子通道而产生的，从而在膜电位上产生局部变化。电场的这种变化会很快受到膜中相邻或较远的离子通道的影响。然后，由于电势变化，这些离子通道可以打开或关闭，从而产生信号。 Cell Meter 荧光细胞电位检测试剂盒使用FRET对（VSB 405和VSR 555）来检测细胞膜电位的变化。亲脂性VSB 405主要位于脂质膜的外层，而VSR 555的定位对细胞膜电位敏感。在静止状态下，的内层具有相对负的电势，从而使VSR 555主要位于细胞膜的外层附近，因此靠近蓝色荧光VSB405，从而导致从蓝色（VSB 405）到蓝色荧光的有效转移。红色（VSR 555）。当细胞去极化时，VSR 555易位到细胞膜的内层，从而分离FRET对并破坏FRET。蓝色/红色荧光比与细胞电位成正比。该检测法可用于筛选调节离子通道的化合物。

图示

图1.使用Cell Meter 荧光细胞电位检测试剂盒检测HeLa细胞中的膜电位。根据试剂盒说明对HeLa细胞进行染色，并用去极化溶液（164.5 mM KCl，2 mM CaCl2、1 mM MgCl2、10 mM葡萄糖，20 mM HEPES，pH 7.4）刺激。使用FlexStation 3（分子设备）记录响应。

参考文献

Assessing Spatiotemporal and Functional Organization of Mitochondrial Networks.
Authors: Kurz, Felix T and Aon, Miguel A and O’Rourke, Brian and Armoundas, Antonis A
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 383-402

Two-photon deep-tissue spatially resolved mitochondrial imaging using membrane potential fluorescence fluctuations.
Authors: Tehrani, Kayvan Forouhesh and Pendleton, Emily G and Southern, William M and Call, Jarrod A and Mortensen, Luke J
Journal: Biomedical optics express (2018): 254-259

Visualizing and Quantifying the Effect of the Inhibition of HSP70 on Breast Cancer Cells Based on Laser Scanning Microscopy.
Authors: Yu, Biying and Yang, Hongqin and Zhang, Xiaoman and Li, Hui
Journal: Technology in cancer research & treatment (2018): 1533033818785274

Amplification of arsenic genotoxicity by TiO2 nanoparticles in mammalian cells: new insights from physicochemical interactions and mitochondria.
Authors: Wang, Xinan and Liu, Yun and Wang, Juan and Nie, Yaguang and Chen, Shaopeng and Hei, Tom K and Deng, Zhaoxiang and Wu, Lijun and Zhao, Guoping and Xu, An
Journal: Nanotoxicology (2017): 978-995

Calcineurin/nuclear factor-κB signaling mediates isoflurane-induced hippocampal neuroinflammation and subsequent cognitive impairment in aged rats.
Authors: Li, Zhengqian and Ni, Cheng and Xia, Chun and Jaw, Joey and Wang, Yujie and Cao, Yiyun and Xu, Mao and Guo, Xiangyang
Journal: Molecular medicine reports (2017): 201-209

The novel isoxazoline ectoparasiticide fluralaner: selective inhibition of arthropod γ-aminobutyric acid- and L-glutamate-gated chloride channels and insecticidal/acaricidal activity.
Authors: Gassel, Michael and Wolf, Christian and Noack, Sandra and Williams, Heike and Ilg, Thomas
Journal: Insect biochemistry and molecular biology (2014): 111-24

Protective role of mitochondrial K-ATP channel and mitochondrial membrane transport pore in rat kidney ischemic postconditioning.
Authors: Zhang, Wei-liang and Zhao, Yan-li and Liu, Xiao-ming and Chen, Jing and Zhang, Dong
Journal: Chinese medical journal (2011): 2191-5

How DASPMI reveals mitochondrial membrane potential: fluorescence decay kinetics and steady-state anisotropy in living cells.
Authors: Ramadass, Radhan and Bereiter-Hahn, Jürgen
Journal: Biophysical journal (2008): 4068-76

A cell-sparing electric field stimulation technique for high-throughput screening of voltage-gated ion channels.
Authors: Bugianesi, Randal M and Augustine, Paul R and Azer, Karim and Dufresne, Claude and Herrington, James and Kath, Gary S and McManus, Owen B and Napolitano, Christopher S and Rush, Alison and Sachs, Jeff and Simpson, Neal and Wismer, Michael K and Kaczorowski, Gregory J and Slaughter, Robert S
Journal: Assay and drug development technologies (2006): 21-35

Bioassay-guided isolation of epiquinamide, a novel quinolizidine alkaloid and nicotinic agonist from an Ecuadoran poison frog, Epipedobates tricolor.
Authors: Fitch, Richard W and Garraffo, H Martin and Spande, Thomas F and Yeh, Herman J C and Daly, John W
Journal: Journal of natural products (2003): 1345-50