产品基本信息

货号：22330

产品名称：FIASH试剂

规格：100 Tests

储存条件：-20℃干燥

保质期：12个月

产品物理化学光谱特性

外观：固体

分子量：664.49

溶剂：DMSO

Ex(nm):498

Em(nm):517

产品介绍

FlASH是一种荧光素衍生物，被修饰为包含两个彼此相距一定距离的砷原子。它由Roger Tsien及其同事在1998年开发。双砷标记技术通过砷与硫醇的高亲和力相互作用起作用。当FlASH结合四半胱氨酸（TC）序列时，其双砷基与Cys-Cys部分快速反应，并且标签变为绿色的高度荧光。双砷标记试剂FIASH是用于标记包含具有Cys-Cys-X1-X2-Cys-Cys氨基酸序列的六氨基酸基序的蛋白质的最小表达标签。最常用的四半胱氨酸是六个氨基酸的Cys-Cys-Pro-Gly-Cys-Cys序列。由于此序列很少出现在内源蛋白中，将序列整合到靶蛋白中会产生一个小的但高度特异性的蛋白标记靶。当与包含四半胱氨酸基序Cys-Cys-Pro-Gly-Cys-Cys的重组蛋白结合时，FIASH会产生强烈的绿色荧光信号。它可以用于检测蛋白质的定位，周转和运输，受体信号传导和内在化。

参考文献

Fluorescent labelling of membrane fatty acid transporter CD36 (SR-B2) in the extracellular loop.
Authors: Liu, Yilin and Rodriguez-Calvo, Ricardo and Wang, Shujin and Zhu, Xiaoqing and Broers, Jos L V and Glatz, Jan F C and Luiken, Joost J F P and Neumann, Dietbert
Journal: PloS one (2019): e0210704

Probing Arrestin Function Using Intramolecular FlAsH-BRET Biosensors.
Authors: Strungs, Erik G and Luttrell, Louis M and Lee, Mi-Hye
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 309-322

Probing the Effect of Sildenafil on Progesterone and Testosterone Production by an Intracellular FRET/BRET Combined Approach.
Authors: Casarini, Livio and Riccetti, Laura and Limoncella, Silvia and Lazzaretti, Clara and Barbagallo, Federica and Pacifico, Salvatore and Guerrini, Remo and Tagliavini, Simonetta and Trenti, Tommaso and Simoni, Manuela and Sola, Marco and Di Rocco, Giulia
Journal: Biochemistry (2019): 799-808

Small Fluorescein Arsenical Hairpin-Based Förster Resonance Energy Transfer Analysis Reveals Changes in Amino- to Carboxyl-Terminal Interactions upon OAG Activation of Classical Transient Receptor Potential 6.
Authors: Fiedler, Susanne and Storch, Ursula and Erdogmus, Serap and Gudermann, Thomas and Mederos Y Schnitzler, Michael and Dietrich, Alexander
Journal: Molecular pharmacology (2019): 90-98

Fluorometric detection of protein-ligand engagement: The case of phosphodiesterase5.
Authors: Di Rocco, Giulia and Martinelli, Ilaria and Pacifico, Salvatore and Guerrini, Remo and Cichero, Elena and Fossa, Paola and Franchini, Silvia and Cardarelli, Silvia and Giorgi, Mauro and Sola, Marco and Ponterini, Glauco
Journal: Journal of pharmaceutical and biomedical analysis (2018): 335-342

Kinetic and Conformational Insights into Islet Amyloid Polypeptide Self-Assembly Using a Biarsenical Fluorogenic Probe.
Authors: Quittot, Noé and Sebastiao, Mathew and Al-Halifa, Soultan and Bourgault, Steve
Journal: Bioconjugate chemistry (2018): 517-527

A sensitive assay reveals structural requirements for α-synuclein fibril growth.
Authors: Dhavale, Dhruva D and Tsai, Christina and Bagchi, Devika P and Engel, Laura A and Sarezky, Jonathan and Kotzbauer, Paul T
Journal: The Journal of biological chemistry (2017): 9034-9050

Characterization of Fluorescein Arsenical Hairpin (FlAsH) as a Probe for Single-Molecule Fluorescence Spectroscopy.
Authors: Fernandes, Dennis D and Bamrah, Jasbir and Kailasam, Senthilkumar and Gomes, Gregory-Neal W and Li, Yuchong and Wieden, Hans-Joachim and Gradinaru, Claudiu C
Journal: Scientific reports (2017): 13063

Detection of oligonucleotides by PNA-peptide conjugates recognizing the biarsenical fluorescein complex FlAsH-EDT2.
Authors: Piras, Linda and Avitabile, Concetta and D’Andrea, Luca Domenico and Saviano, Michele and Romanelli, Alessandra
Journal: Biochemical and biophysical research communications (2017): 126-131

Probing amylin fibrillation at an early stage via a tetracysteine-recognising fluorophore.
Authors: Wang, Shih-Ting and Lin, Yiyang and Hsu, Chia-Chen and Amdursky, Nadav and Spicer, Christopher D and Stevens, Molly M
Journal: Talanta (2017): 44-50