产品基本信息

货号：22332

产品名称：REASH试剂

规格：100 Tests

储存条件：-20℃干燥

保质期：12个月

产品物理化学光谱特性

外观：固体

分子量：545.37

溶剂：DMSO

Ex(nm):571

Em(nm):584

产品介绍

REASH是一种试卤灵衍生物，经修饰可包含两个彼此相距一定距离的砷原子。双砷标记技术通过砷与硫醇的高亲和力相互作用起作用。当REASH结合四半胱氨酸序列时，其双砷基团与Cys-Cys部分快速反应，标签变成红色的高度荧光。双砷标记试剂REASH是用于标记蛋白质的最小表达标签之一，该蛋白质包含具有Cys-Cys-X1-X2-Cys-Cys氨基酸序列的六氨基酸基序。最常用的四半胱氨酸是六个氨基酸的Cys-Cys-Pro-Gly-Cys-Cys序列。由于该序列很少出现在内源蛋白中，因此将该序列整合到靶蛋白中会生成一个小的但高度特异性的蛋白标记靶。当与含有四半胱氨酸基序Cys-Cys-Pro-Gly-Cys-Cys的重组蛋白结合时，REASH会产生强烈的红色荧光信号。它可以用于监测蛋白质的定位，周转和运输，受体信号传导和内在化。

参考文献

Discrete Virus Factories Form in the Cytoplasm of Cells Coinfected with Two Replication-Competent Tagged Reporter Birnaviruses That Subsequently Coalesce over Time.
Authors: Campbell, Elle A and Reddy, Vishwanatha R A P and Gray, Alice G and Wells, Joanna and Simpson, Jennifer and Skinner, Michael A and Hawes, Philippa C and Broadbent, Andrew J
Journal: Journal of virology (2020)

Tetracysteine-based fluorescent tags to study protein localization and trafficking in Plasmodium falciparum-infected erythrocytes.
Authors: Crivat, Georgeta and Tokumasu, Fuyuki and Sa, Juliana Martha and Hwang, Jeeseong and Wellems, Thomas E
Journal: PloS one (2011): e22975

Hairpin structure of a biarsenical-tetracysteine motif determined by NMR spectroscopy.
Authors: Madani, Fatemeh and Lind, Jesper and Damberg, Peter and Adams, Stephen R and Tsien, Roger Y and Gräslund, Astrid O
Journal: Journal of the American Chemical Society (2009): 4613-5

Helix A stabilization precedes amino-terminal lobe activation upon calcium binding to calmodulin.
Authors: Chen, Baowei and Lowry, David F and Mayer, M Uljana and Squier, Thomas C
Journal: Biochemistry (2008): 9220-6

Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins.
Authors: Adams, Stephen R and Tsien, Roger Y
Journal: Nature protocols (2008): 1527-34

New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications.
Authors: Adams, Stephen R and Campbell, Robert E and Gross, Larry A and Martin, Brent R and Walkup, Grant K and Yao, Yong and Llopis, Juan and Tsien, Roger Y
Journal: Journal of the American Chemical Society (2002): 6063-76

Identification of an orthogonal peptide binding motif for biarsenical multiuse affinity probes.
Authors: Chen, Baowei and Cao, Haishi and Yan, Ping and Mayer, M Uljana and Squier, Thomas C
Journal: Bioconjugate chemistry: 1259-65

Tetracysteine genetic tags complexed with biarsenical ligands as a tool for investigating gap junction structure and dynamics.
Authors: Sosinsky, Gina E and Gaietta, Guido M and Hand, Galen and Deerinck, Thomas J and Han, Areum and Mackey, Mason and Adams, Stephen R and Bouwer, James and Tsien, Roger Y and Ellisman, Mark H
Journal: Cell communication & adhesion: 181-6