上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
FDGlcU 荧光素二-β-D-葡糖醛酸(停产)
货号 | 14002 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 1 mg | 价格 | 0 | |
Ex (nm) | 498 | Em (nm) | 517 | |
分子量 | 684.55 | 溶剂 | DMSO | |
产品详细介绍 |
简要概述
产品基本信息
货号:14002
产品名称:FDGlcU 荧光素二-β-D-葡糖醛酸(停产)
规格:1mg
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
分子量:684.55
溶剂:DMSO
激发波长(nm):490
发射波长(nm):514
产品介绍
FDGlcU 荧光素二-β-D-葡糖醛酸是美国AAT Bioquest生产的用于检测FDGlcU的试剂,来自于大肠杆菌的β-葡萄糖苷酸酶(GUS),被用于转基因植物中具有优良特性的标志基因。GUS报告基因系统具有很多优势,如稳定表达的大肠杆菌的GUS酶对正常植物代谢无干扰,而高等植物中具有低背景的GUS活性。FDGlcU被认为是测定GUS酶最敏感的荧光底物之一。无色且非荧光的FDGlcU 被水解成高荧光的荧光素,它可以展现出完美的光谱特性(Ex/Em=492/520nm),与大多数荧光检测设备的最佳检测窗口所匹配。葡萄糖苷酸酶催化水解FDGlcU ,并在520nm处产生荧光。此外,由于FDGlcU的产物,荧光素具有强大的消光系数(100,000 cm-1mol-1),FDGlcU也被作为显色底物用于在发光模式下检测葡萄糖苷酸酶的活性。通过荧光显微镜或者流式细胞仪FDGlcU也能用来识别GUS阳性表达细胞。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的FDGlcU 荧光素二-β-D-葡糖醛酸。
点击查看光谱
参考文献
Active site tyrosine is essential for amidohydrolase but not for esterase activity of a class 2 histone deacetylase-like bacterial enzyme
Authors: Moreth K, Riester D, Hildmann C, Hempel R, Wegener D, Schober A, Schwienhorst A.
Journal: Biochem J. (2006)
Arabidopsis thaliana histone deacetylase 1 (AtHD1) is localized in euchromatic regions and demonstrates histone deacetylase activity in vitro
Authors: Fong PM, Tian L, Chen ZJ.
Journal: Cell Res (2006): 479
DNA damage promotes histone deacetylase 4 nuclear localization and repression of G2/M promoters, via p53 C-terminal lysines
Authors: Basile V, Mantovani R, Imbriano C.
Journal: J Biol Chem (2006): 2347
Enhanced transgene expression in urothelial cancer gene therapy with histone deacetylase inhibitor Okegawa T, Nutahara K, Pong RC, Higashihara E, Hsieh JT. Department of Urology, University of Kyorin, Tokyo, Japan
Authors: Hsieh JT.
Journal: Urol Oncol (2006): 565
Experimental therapy of malignant gliomas using the inhibitor of histone deacetylase MS-275
Authors: Eyupoglu IY, Hahnen E, Trankle C, Savaskan NE, Siebzehnrubl FA, Buslei R, Lemke D, Wick W, Fahlbusch R, Blumcke I.
Journal: Mol Cancer Ther (2006): 1248
Fetal hemoglobin induction by histone deacetylase inhibitors involves generation of reactive oxygen species
Authors: Hsiao CH, Li W, Lou TF, Baliga BS, Pace BS.
Journal: Exp Hematol (2006): 264
Histone deacetylase (HDAC) 4 involvement in both Lewy and Marinesco bodies
Authors: Takahashi-Fujigasaki J, Fujigasaki H.
Journal: Neuropathol Appl Neurobiol (2006): 562
Histone deacetylase inhibitor FR901228 enhances the antitumor effect of telomerase-specific replication-selective adenoviral agent OBP-301 in human lung cancer cells
Authors: Watanabe T, Hioki M, Fujiwara T, Nishizaki M, Kagawa S, Taki M, Kishimoto H, Endo Y, Urata Y, Tanaka N.
Journal: Exp Cell Res (2006): 256
Induction of apoptosis and inhibition of telomerase activity by trichostatin A, a histone deacetylase inhibitor, in human leukemic U937 cells
Authors: Woo HJ, Lee SJ, Choi BT, Park YM, Choi YH.
Journal: Exp Mol Pathol. (2006)
Real-time gene expression analysis in human xenografts for evaluation of histone deacetylase inhibitors
Authors: Belien A, De Schepper S, Floren W, Janssens B, Marien A, King P, Van Dun J, Andries L, Voeten J, Bijnens L, Janicot M, Arts J.
Journal: Mol Cancer Ther (2006): 2317