AF 488醛 货号9015-AAT Bioquest荧光染料

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AF 488醛

AF 488醛

AF 488醛    货号9015 货号 9015 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 4992
Ex (nm) 499 Em (nm) 520
分子量 750.75 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:9015

产品名称:AF 488醛

规格:5 mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:639.02

溶剂:DMSO

激发波长(nm):499

发射波长(nm):520

消光系数(cm-1 M-1):73000

荧光量子产率:0.92

校正因子 (280 nm):0.11

 

产品介绍

AF488醛包含AlexaFluor®488的荧光团。它是一种可与胺,肼或羟胺反应的活性绿色荧光染料。醛基可与pH 5-9的胺,酰肼或羟胺基反应。与胺反应性琥珀酰亚胺基酯基(NHS)不同,醛可以在酸性pH下与N端胺基反应,这是某些生物共轭反应有时需要的条件。醛与胺基反应形成席夫碱。用氢化物进一步还原将形成稳定的C-N键。醛与其他基团之间的反应可实现荧光素染料的位点特异性缀合和标记,使其靶向分子上的所需位置。可以通过普通的荧光仪器在FITC通道下轻松检测到共轭AF488染料。 AlexaFluor®488是ThermoFisher的商标。

点击查看光谱

 

参考文献

Optimization of 3-aminopropyltriethoxysilane functionalization on silicon nitride surface for biomolecule immobilization.
Authors: Saengdee, Pawasuth and Promptmas, Chamras and Thanapitak, Surachoke and Srisuwan, Awirut and Pankiew, Apirak and Thornyanadacha, Nutthaphat and Chaisriratanakul, Woraphan and Chaowicharat, Ekalak and Jeamsaksiri, Wutthinan
Journal: Talanta (2020): 120305

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments.
Authors: Grate, Jay W and Mo, Kai-For and Shin, Yongsoon and Vasdekis, Andreas and Warner, Marvin G and Kelly, Ryan T and Orr, Galya and Hu, Dehong and Dehoff, Karl J and Brockman, Fred J and Wilkins, Michael J
Journal: Bioconjugate chemistry (2015): 593-601

Simultaneous visualization and cell-specific confirmation of RNA and protein in the mouse retina.
Authors: Stempel, Andrew J and Morgans, Catherine W and Stout, J Timothy and Appukuttan, Binoy
Journal: Molecular vision (2014): 1366-73

Amine-reactive pyridylhydrazone-based PEG reagents for pH-reversible PEI polyplex shielding.
Authors: Fella, Carolin and Walker, Greg F and Ogris, Manfred and Wagner, Ernst
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2008): 309-20

Raman nanoparticle probes for antibody-based protein detection in tissues.
Authors: Lutz, Barry and Dentinger, Claire and Sun, Lei and Nguyen, Lienchi and Zhang, Jingwu and Chmura, Aj and Allen, April and Chan, Selena and Knudsen, Beatrice
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2008): 371-9

Rapid oxime and hydrazone ligations with aromatic aldehydes for biomolecular labeling.
Authors: Dirksen, Anouk and Dawson, Philip E
Journal: Bioconjugate chemistry (2008): 2543-8

Oligonucleotide-arrayed TFT photosensor applicable for DNA chip technology.
Authors: Tanaka, Tsuyoshi and Hatakeyama, Keiichi and Sawaguchi, Masahiro and Iwadate, Akihito and Mizutani, Yasushi and Sasaki, Kazuhiro and Tateishi, Naofumi and Takeyama, Haruko and Matsunaga, Tadashi
Journal: Biotechnology and bioengineering (2006): 22-8

Use of anti-fluorophore antibody to achieve high-sensitivity immunolocalizations of transporters and ion channels.
Authors: Coleman, Richard A and Liu, Jie and Wade, James B
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2006): 817-27

Acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils.
Authors: Amraei, Mohammad and Jia, Zongjian and Reboul, Pascal and Nabi, Ivan R
Journal: The Journal of biological chemistry (2003): 38935-41

Lipopolysaccharide (LPS) labeled with Alexa 488 hydrazide as a novel probe for LPS binding studies.
Authors: Triantafilou, K and Triantafilou, M and Fernandez, N
Journal: Cytometry (2000): 316-20

说明书
AF 488醛.pdf