上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
荧光素酶 重组荧光素
货号 | 12500 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 1 mL | 价格 | 1272 | |
Ex (nm) | Em (nm) | |||
分子量 | ~60 kDa | 溶剂 | Water | |
产品详细介绍 |
简要概述
产品基本信息
货号:12500
产品名称:荧光素酶 重组荧光素
CAS:61970-00-1
规格:1ml
储存条件:-15℃避光防潮
保质期:24个月
产品介绍
荧光素酶 重组荧光素 是美国AAT Bioquest生产的荧光素酶,该酶是由来自北美萤火虫(Photinus pyralis)的克隆基因表达的荧光素酶,其可以使用生物发光试剂检测ATP或荧光素底物进行研究或生产试剂盒。 这种重组酶有可能消除从天然来源纯化的荧光素酶中发现的季节性和区域性变异性。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的荧光素酶 重组荧光素 。
金畔问号:荧光素酶到底该如何检测?
图1.在带有NOVOstar酶标仪(BMG Labtech)的白色96孔板中,使用Amplite 荧光素酶报告基因基因检测试剂盒检测了荧光素酶活性。 孵育20分钟后,仍可检测到荧光素酶活性为0.1 pg /孔。
参考文献
Measuring HIV Neutralization in a Luciferase Reporter Gene Assay
Authors: Montefiori DC.
Journal: Methods Mol Biol (2009): 395
A cell-based luciferase assay amenable to high-throughput screening of inhibitors of arenavirus budding
Authors: Capul AA, de la Torre JC.
Journal: Virology (2008): 107
An optimized luciferase bioluminescent assay for coenzyme A
Authors: Marques SM, da Silva JC.
Journal: Anal Bioanal Chem (2008): 2161
Application of the dual-luciferase reporter assay to the analysis of promoter activity in Zebrafish embryos
Authors: Alcaraz-Perez F, Mulero V, Cayuela ML.
Journal: BMC Biotechnol (2008): 81
Firefly luciferase complementation imaging assay for protein-protein interactions in plants
Authors: Chen H, Zou Y, Shang Y, Lin H, Wang Y, Cai R, Tang X, Zhou JM.
Journal: Plant Physiol (2008): 368
Quantitation of autophagy by luciferase release assay
Authors: Ketteler R, Seed B.
Journal: Autophagy (2008): 801
Quantitative assessment of the knockdown efficiency of morpholino antisense oligonucleotides in zebrafish embryos using a luciferase assay
Authors: Kamachi Y, Okuda Y, Kondoh H.
Journal: Genesis (2008): 1
Determination of silencing potency of synthetic and RNase III-generated siRNA using a secreted luciferase assay
Authors: Morlighem JE, Petit C, Tzertzinis G.
Journal: Biotechniques (2007): 599
A luciferase-based budding assay for Ebola virus
Authors: McCarthy SE, Licata JM, Harty RN.
Journal: J Virol Methods (2006): 115
Luciferase reporter assay
Authors: Wettey FR, Jackson AP.
Journal: Subcell Biochem (2006): 423