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Fura-10，钾盐
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货号 | 21110 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 5×50 ug | 价格 | 1200 | |
| Ex (nm) | 354 | Em (nm) | 524 | |
| 分子量 | 1024.27 | 溶剂 | Water | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：21110
产品名称：Fura-10，钾盐
规格：5×50 ug
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
分子量：1024.27
溶剂：水
产品介绍
与Fluo-4等单波长探针不同，对于Ca2+，Fura探针的最大吸收（或荧光激发）从380 nm（Fura-2），415 nm（Fura-8和Fura-10）转变，与Ca2+结合的无螯合剂可达到约340 nm（Fura-2），355 nm（Fura-8和Fura-10），最大荧光发射的波长相对独立于Ca2+浓度。通过使用在340nm和380nm（对于Fura-2），355nm和415nm（对于Fura-8和Fura-10）进行激发并分配荧光强度来获得Ca2+依赖性荧光信号的最大动态范围在〜510 nm（Fura-2），525 nm（Fura-8和Fura-10）处检测到。根据该比率，可以使用从校准曲线得出的解离常数（Kd）估算细胞内Ca2+的水平。通过使用在两个波长下激发产生的荧光强度之比，诸如染料分布不均匀和光致漂白等因素将被最小化，因为它们应在相同程度上影响两种测量。校准溶液最初应不含重金属离子，例如锰，这可能会影响其荧光和对钙的亲和力。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Fura-10，钾盐。
参考文献
A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells.
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Ho, Quynh Mai and Leem, Chae Hun
Journal: Journal of visualized experiments : JoVE (2019)
Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2.
Authors: Johnson, Martin
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 163-172
Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular Cd2+ in a pancreatic beta cell line.
Authors: Malaiyandi, Latha M and Sharthiya, Harsh and Barakat, Ameir N and Edwards, Joshua R and Dineley, Kirk E
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2019): 951-964
A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision.
Authors: Tinning, P W and Franssen, A J P M and Hridi, S U and Bushell, T J and McConnell, G
Journal: Journal of microscopy (2018): 212-220
Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-fura-2 and Analogues.
Authors: Zhang, Guangqian and Jacquemin, Denis and Buccella, Daniela
Journal: The journal of physical chemistry. B (2017): 696-705
Heavy metal chelator TPEN attenuates fura-2 fluorescence changes induced by cadmium, mercury and methylmercury.
Authors: Ohkubo, Masato and Miyamoto, Atsushi and Shiraishi, Mitsuya
Journal: The Journal of veterinary medical science (2016): 761-7
Interferences of resveratrol with fura-2-derived fluorescence in intracellular free-Ca(2+) concentration determinations.
Authors: Santofimia-Castaño, Patricia and Salido, Gines M and Gonzalez, Antonio
Journal: Cytotechnology (2016): 1369-80
A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca(2+) Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat.
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Leem, Chae Hun
Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology (2015): 373-82
Resveratrol Interferes with Fura-2 Intracellular Calcium Measurements.
Authors: Kopp, Richard F and Leech, Colin A and Roe, Michael W
Journal: Journal of fluorescence (2014): 279-84
Resveratrol is not compatible with a Fura-2-based assay for measuring intracellular Ca²⁺ signaling.
Authors: Paudel, Ram Chandra and Kiviluoto, Santeri and Parys, Jan B and Bultynck, Geert
Journal: Biochemical and biophysical research communications (2014): 1626-30