harlan teklad官网TD.02028 TD.02028 TD.96121 动物饲料 胆固醇和胆酸盐来源的纯化高脂饮食

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harlan teklad官网TD.02028 TD.02028 TD.96121 动物饲料 胆固醇和胆酸盐来源的纯化高脂饮食

野生型小鼠和大鼠一般对动脉粥样硬化有抵抗力，需要更极端的饮食操作来改变脂蛋白谱，形成轻度动脉粥样硬化(泡沫细胞、脂肪条纹)。现代配方完全是由纯化成分制成的，因为据报道，这种更精细的方法可以减少较少精制和较传统饮食方法引起的胆结石和肝脏损害的发生率。为了在野生型动物中诱导轻度动脉粥样硬化，可对“西方”纯化饲料进行改良，以提高胆固醇(1-1.25%)，并添加胆酸钠或胆酸等胆盐。联系我们获得更多的信息，修改，或可能的控制饮食。

添加胆固醇和胆酸盐来源的纯化高脂饮食的例子*：

  • TD.0202821%乳脂(1.25%胆固醇，0.5%胆酸)
  • TD.0923715%乳脂饮食(1%胆固醇，0.5%胆酸钠)

添加胆固醇的纯化高脂饮食的例子(没有胆酸来源)：

研究用途：

主要在野生型小鼠和大鼠诱发高胆固醇血症和轻度动脉粥样硬化(泡沫细胞、脂肪条纹)。

不会助长肥胖。

主要饮食特征：

  • 高脂饮食(体重15-20%；脂肪34-45千卡)
  • 饱和脂肪酸(SFA>总脂肪酸的55%)
  • 乳脂/黄油、可可脂
  • 蔗糖(重量占30%-50%)
  • 胆固醇(1-1.25%)
  • 巧克力源(0.5%)*

参考资料：

  1. 番茄汁番茄红素对大鼠肝脏脂肪变性的影响。j Nutr Biochem，2013年。24(11)：P。1870-81
  2. 高，Q，等，动脉粥样硬化饮食加剧了小鼠缺乏谷胱甘肽过氧化物酶结肠炎。炎症肠Dis，2010年。16(12)：P。2043-54
  3. Lichtman，A.H.，等，高脂血症和动脉粥样硬化病变的发展，低密度脂蛋白受体缺乏的小鼠，喂食定义半纯化饮食与不含胆酸。Arteroscler Thromb VASc Biol，1999年。19(8)：P。1938-44年。
  4. 慢性精神应激和致动脉粥样硬化饮食对小鼠主动脉免疫炎症环境的影响。BrainBehav Immun，2011年。25(8)：P。1649-57
  5. Nishina，P.m.，等，动物和植物来源的饮食脂肪对C57BL/6J小鼠因饮食引起的脂肪条纹损害的影响。j Lipid RES，1993年。34(8)：P。1413-22
  6. Nishina，P.m.，等，9种近交系小鼠动脉粥样硬化及血浆和肝脏脂质。脂质，1993年。28(7)：P。599-605
  7. 月，P，等，增强CD 36空小鼠肝脏载脂蛋白A-I分泌及胆固醇和磷脂的外周流出。PLOS One，2010年。5(3)：P。E 9906
  8. Nishina，下午，J.Verstuyft，B.Paigen，合成低脂和高脂肪饮食，用于研究小鼠动脉粥样硬化。j Lipid RES，1990年。31(5)：P。859-69

*胆酸钠或胆酸有助胆固醇和脂肪的吸收，并可透过胆汁酸合成减少胆固醇的排出。然而，如果你的研究不想包括胆酸盐的来源，没有胆酸盐的饮食是可用的。看见TD.96121为了纯正的饮食TD.94059混合饮食。请与我们联系，了解其他选项。

Wild type mice and rats generally are resistant to atherosclerosis, requiring more extreme dietary manipulation to modify lipoprotein profiles and develop mild atherosclerosis (foam cells, fatty streaks). Modern formulations are made completely of purified ingredients because this more refined approach has been reported to decrease the incidence of gallstones and liver damage associated with less refined and more traditional dietary approaches. To induce mild atherosclerosis in wild type animals, the “Western” purified diet can be modified to increase cholesterol (1-1.25%) and add a bile salt such as sodium cholate or cholic acid. Contact us for more information, modifications, or possible control diets.

Examples of purified high fat diets with added cholesterol and cholate source*:

  • TD.02028    21% milkfat (1.25% cholesterol, 0.5% cholic acid)
  • TD.09237    15% milkfat diet (1% cholesterol, 0.5% sodium cholate)

Examples of purified high fat diets with added cholesterol (without cholate source):

  • TD.96121    21% milkfat (1.25% cholesterol)

Research Use:

Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) primarily in wild type mice and rats.

Will not promote obesity.

Key dietary features:

  • High fat diet (15-20% by weight; 34 – 45% kcal from fat)
  • Saturated fatty acids (SFA >55% of total fatty acids)
  • Milkfat/butterfat, cocoa butter
  • Sucrose (30-50% by weight)
  • Cholesterol (1 – 1.25%)
  • Cholate source (0.5%)*

References:

  1. Bernal, C., et al., Lipid biomarkers and metabolic effects of lycopene from tomato juice on liver of rats with induced hepatic steatosis. J Nutr Biochem, 2013. 24(11): p. 1870-81.
  2. Gao, Q., et al., Atherogenic diets exacerbate colitis in mice deficient in glutathione peroxidase. Inflamm Bowel Dis, 2010. 16(12): p. 2043-54.
  3. Lichtman, A.H., et al., Hyperlipidemia and atherosclerotic lesion development in LDL receptor-deficient mice fed defined semipurified diets with and without cholate. Arterioscler Thromb Vasc Biol, 1999. 19(8): p. 1938-44.
  4. Marcondes, M.C., et al., Effects of chronic mental stress and atherogenic diet on the immune inflammatory environment in mouse aorta. Brain Behav Immun, 2011. 25(8): p. 1649-57.
  5. Nishina, P.M., et al., Effects of dietary fats from animal and plant sources on diet-induced fatty streak lesions in C57BL/6J mice. J Lipid Res, 1993. 34(8): p. 1413-22.
  6. Nishina, P.M., et al., Atherosclerosis and plasma and liver lipids in nine inbred strains of mice. Lipids, 1993. 28(7): p. 599-605.
  7. Yue, P., et al., Enhanced hepatic apoA-I secretion and peripheral efflux of cholesterol and phospholipid in CD36 null mice. PLoS One, 2010. 5(3): p. e9906.
  8. Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res, 1990. 31(5): p. 859-69.

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis. However, if including a cholate source is not desired for your research, diets without cholate are available. See TD.96121 for a purified diet and TD.94059 for a hybrid diet. Contact us for additional options.