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AF488 醛 *与 Alexa Fluor™ 488 醛的结构相同*
货号 | 9016 | 存储条件 | ||
规格 | 1 mg | 价格 | 3612 | |
Ex (nm) | 499 | Em (nm) | 520 | |
分子量 | 750.75 | 溶剂 | ||
产品详细介绍 |
简要概述
产品基本信息
货号:9016
产品名称:AF488 醛 *与 Alexa Fluor™ 488 醛的结构相同*
规格:1mg
储存条件:保存在冰箱-15℃干燥
保质期:12个月
产品物理化学光谱特性
分子量:750.75
外观:固体
溶剂:DMSO
激发波长(nm):499
发射波长(nm):520
产品介绍
AF488 由 AAT Bioquest 生产,与 Alexa Fluor® 488 具有相同的化学结构(Alexa Fluor® 是 ThermoFisher 的商标)。 AF488 醛含有 XFD488 的荧光团。 它是一种活性绿色荧光染料,可与胺、肼或羟胺反应。 醛基对 pH 5-9 的胺、酰肼或羟胺基具有反应性。 与胺反应性琥珀酰亚胺酯基团 (NHS) 不同,醛可以在酸性 pH 下与 N 端胺基反应,某些生物偶联反应有时需要这种条件。 醛与胺基反应形成中间席夫键。 用氢化物进一步还原将形成稳定的 C-N 键。 醛和其他基团之间的反应允许荧光素染料在目标分子上的所需位置进行位点特异性结合和标记。 AF488 染料可以通过 FITC 通道下的普通荧光仪器轻松检测。
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参考文献
Optimization of 3-aminopropyltriethoxysilane functionalization on silicon nitride surface for biomolecule immobilization.
Authors: Saengdee, Pawasuth and Promptmas, Chamras and Thanapitak, Surachoke and Srisuwan, Awirut and Pankiew, Apirak and Thornyanadacha, Nutthaphat and Chaisriratanakul, Woraphan and Chaowicharat, Ekalak and Jeamsaksiri, Wutthinan
Journal: Talanta (2020): 120305
Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments.
Authors: Grate, Jay W and Mo, Kai-For and Shin, Yongsoon and Vasdekis, Andreas and Warner, Marvin G and Kelly, Ryan T and Orr, Galya and Hu, Dehong and Dehoff, Karl J and Brockman, Fred J and Wilkins, Michael J
Journal: Bioconjugate chemistry (2015): 593-601
Simultaneous visualization and cell-specific confirmation of RNA and protein in the mouse retina.
Authors: Stempel, Andrew J and Morgans, Catherine W and Stout, J Timothy and Appukuttan, Binoy
Journal: Molecular vision (2014): 1366-73
Amine-reactive pyridylhydrazone-based PEG reagents for pH-reversible PEI polyplex shielding.
Authors: Fella, Carolin and Walker, Greg F and Ogris, Manfred and Wagner, Ernst
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2008): 309-20
Raman nanoparticle probes for antibody-based protein detection in tissues.
Authors: Lutz, Barry and Dentinger, Claire and Sun, Lei and Nguyen, Lienchi and Zhang, Jingwu and Chmura, Aj and Allen, April and Chan, Selena and Knudsen, Beatrice
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2008): 371-9
Rapid oxime and hydrazone ligations with aromatic aldehydes for biomolecular labeling.
Authors: Dirksen, Anouk and Dawson, Philip E
Journal: Bioconjugate chemistry (2008): 2543-8
Oligonucleotide-arrayed TFT photosensor applicable for DNA chip technology.
Authors: Tanaka, Tsuyoshi and Hatakeyama, Keiichi and Sawaguchi, Masahiro and Iwadate, Akihito and Mizutani, Yasushi and Sasaki, Kazuhiro and Tateishi, Naofumi and Takeyama, Haruko and Matsunaga, Tadashi
Journal: Biotechnology and bioengineering (2006): 22-8
Use of anti-fluorophore antibody to achieve high-sensitivity immunolocalizations of transporters and ion channels.
Authors: Coleman, Richard A and Liu, Jie and Wade, James B
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2006): 817-27
Acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils.
Authors: Amraei, Mohammad and Jia, Zongjian and Reboul, Pascal and Nabi, Ivan R
Journal: The Journal of biological chemistry (2003): 38935-41
Lipopolysaccharide (LPS) labeled with Alexa 488 hydrazide as a novel probe for LPS binding studies.
Authors: Triantafilou, K and Triantafilou, M and Fernandez, N
Journal: Cytometry (2000): 316-20